Abstract
Alternative splicing or expression from an alternate promoter can produce variants of a gene. To determine whether the p21Waf1/Cip1 locus is regulated by these mechanisms, we searched for and found two transcripts, p21B and p21C, that are expressed from an alternate promoter in the first intron of the p21 gene. While p21C encodes the p21 cyclin-dependent kinase inhibitor, p21B encodes a novel protein and the transcript is ubiquitously expressed in 16 human tissues tested. Like p21, both p21B and p21C are induced by DNA damage, p53, and other p53 family members through a proximal p53 response element in the promoter of p21B and p21C. However, unlike p21, which induces cell cycle arrest, we found that overexpression of p21B induces apoptosis. These findings indicate that the p21 locus expresses at least two structurally distinct, but functionally related, variants of the p21 gene from discrete promoters.
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Acknowledgements
We would like to thank Jianhui Zhu for technical help, Bert Vogelstein for 8OS14 and HCT116 p53−/− cell lines, Charles Di Como and Carol Prives for p63 plasmids, and Rhea Markowitz, and Michael Dohn for critical reading of this manuscript. This work is supported in part by NIH Grant CA76069 and CA81237.