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Molecular cytogenetic analysis of 10;11 rearrangements in acute myeloid leukemia

Leukemia volume 16, pages 344–351 (2002)Cite this article

Abstract

MLLT10 (previously called AF10) is a moderately common MLL fusion partner predominantly occurring in acute monoblastic leukemia (AML-M5). 10;11 rearrangements require at least three breaks in order to generate an in-frame MLL-MLLT10 fusion as a result of the opposite orientations of both genes on the respective chromosome arms. In this study, we describe a detailed molecular cytogenetic analysis of MLL-MLLT10 positive 10;11 rearrangements in two patients. We observed an as yet unreported chromosomal mechanism with at least four breakpoints, leading to MLL-MLLT10 gene fusion in a 24-year-old male. An inversion of 11q13-q23 with a breakpoint in the MLL gene was followed by an additional break 3′ of MLL prior to insertion of the 11q segment into MLLT10. In a second patient, a 37-year-old male with AML-M5b, molecular cytogenetic analysis of an apparent 10;11 reciprocal translocation showed an intrachromosomal inversion of 3′MLLT10followed by a reciprocal translocation between 10p12 and 11q23. Review of the literature showed that all cases were the result of an inversion of either 10p or 11q followed by translocation 10p;11q or insertion of the inverted segment into MLLT10 or MLL.

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Acknowledgements

FWO grant Nos G.0028.00 and G.0310.01 and BOF grant No. 011D7699. Heidi Van Limbergen is the recipient of a research grant of the University of Ghent. Bruce Poppe is supported by a grant of the Fund for Scientific Research, Flanders, Belgium (FWO-Vlaanderen).

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Authors and Affiliations

  1. Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium

    H Van Limbergen, B Poppe, A De Paepe & F Speleman

  2. Department of Hematology, Ghent University Hospital, Ghent, Belgium

    A Janssens & L Noens

  3. Department of Hematology, AZ Middelheim, Antwerp, Belgium

    R De Bock

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Van Limbergen, H., Poppe, B., Janssens, A. et al. Molecular cytogenetic analysis of 10;11 rearrangements in acute myeloid leukemia. Leukemia 16, 344–351 (2002). https://doi.org/10.1038/sj.leu.2402397

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