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Immune Recovery

Hematopoietic reconstitution after lethal irradiation and bone marrow transplantation: effects of different hematopoietic cytokines on the recovery of thymus, spleen and blood cells

Bone Marrow Transplantation volume 25, pages 427–433 (2000)Cite this article

Abstract

Lethally irradiated mice were grafted with syngeneic bone marrow cells or left ungrafted. Mice of each group were injected with different hematopoietic cytokines for 5 consecutive days starting immediately after irradiation or left uninjected. The recovery of lymphoid tissues induced by hematopoietic cytokines 7 days after irradiation and bone marrow cell transplantation was comparable to that observed at days 21–28 in irradiated, bone marrow-grafted, but cytokine-uninjected mice. IL-11 or IL-6, in combination with IL-3, was able to hasten thymus, spleen and blood cell numbers and functions. SCF also displayed a detectable effect when used with IL-3. Conversely, the IL-6 super-agonist K-7/D-6 was able, when injected alone, to induce significant recovery of thymus, spleen and blood cells. Thus, K-7/D-6 appears to be a most efficient cytokine for fast reconstitution of lymphoid tissues after irradiation and bone marrow transplantation. Bone Marrow Transplantation (2000) 25, 427–433.

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Acknowledgements

We thank Dr S Neben (Genetics Institute) for kindly providing IL-11, and Dr G Ciliberto (IRBMP Angeletti) for the gifts of hu r IL-6, K-7/D-6 and human soluble IL-6R. This work was supported in part by CNR Progetto Finalizzato Biotecnologie.

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  1. Laboratory of Immunology, AMB-PRO-TOSS, ENEA CR Casaccia, Rome, Italy

    D Frasca, F Guidi, M Arbitrio & C Pioli

  2. Department of Biology, University of Rome ‘Tor Vergata’, Rome, Italy

    F Poccia, R Cicconi & G Doria

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  1. D Frasca
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Frasca, D., Guidi, F., Arbitrio, M. et al. Hematopoietic reconstitution after lethal irradiation and bone marrow transplantation: effects of different hematopoietic cytokines on the recovery of thymus, spleen and blood cells. Bone Marrow Transplant 25, 427–433 (2000). https://doi.org/10.1038/sj.bmt.1702169

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