New nano-complexes targeting protein 3S7S in breast cancer and protein 4OO6 in liver cancer investigated in cell line

Cancer, a lethal ailment, possesses a multitude of therapeutic alternatives to combat its presence, metal complexes have emerged as significant classes of medicinal compounds, exhibiting considerable biological efficacy, especially as anticancer agents. The utilization of cis-platin in the treatment of various cancer types, including breast cancer, has served as inspiration to devise novel nanostructured metal complexes for breast cancer therapy. Notably, homo- and hetero-octahedral bimetallic complexes of an innovative multifunctional ether ligand (comprising Mn(II), Ni(II), Cu(II), Zn(II), Hg(II), and Ag(I) ions) have been synthesized. To ascertain their structural characteristics, elemental and spectral analyses, encompassing IR, UV–Vis, 1H-NMR, mass and electron spin resonance (ESR) spectra, magnetic moments, molar conductance, thermal analysis, and electron microscopy, were employed. The molar conductance of these complexes in DMF demonstrated a non-electrolytic nature. Nanostructured forms of the complexes were identified through electron microscopic data. At ambient temperature, the ESR spectra of the solid complexes exhibited anisotropic and isotropic variants, indicative of covalent bonding. The ligand and several of its metal complexes were subjected to cytotoxicity testing against breast cancer protein 3S7S and liver cancer protein 4OO6, with the Ag(I) complex (7) evincing the most potent effect, followed by the Cu(II) with ligand (complex (2)), Cis-platin, the ligand itself, and the Cu(II)/Zn(II) complex (8). Molecular docking data unveiled the inhibitory order of several complexes.

by metal ions have significantly contributed to the advancement of therapeutic approaches based on metal compounds.Cisplatin, a prominent medication derived from metals, is extensively utilized in the management of cancer, specifically in the treatment of genitourinary tumors like testicular cancer 1,2 .Metal-based chemotherapeutic agents have been the subject of investigation for potential medical applications.The extensive knowledge possessed by inorganic chemists regarding the coordination and redox properties of metal ions has greatly contributed to the advancement of modern medicinal inorganic chemistry.This field was further stimulated by the fortuitous discovery of cisplatin.Following the identification of suitable ligands for metal ion binding, the characteristics and role of nucleic acids in metal complex formation have been elucidated.Recent reviews have provided a comprehensive overview of the accomplishments in the utilization of metal complexes for medical purposes [3][4][5] .There has been a growing demand for metal-based compounds in cancer treatment.This surge in demand can be attributed to the prevalence of cancer and, to a lesser extent, the demonstrated in vitro cytotoxic activity of recently synthesized metal-based compounds 6,7 .Schiff base derivatives with a 4-acyl-2-pyrazolin-5-one moiety are a significant class of chemical molecules due to their structural chemistry and biological activity 7,8 .In the realm of anticancer research, pyrazolines have exhibited promising antiproliferative effects against human myelogenous leukemia HL-606.The coordinating ability of the 4-amino-2,3-dimethyl-1-phenyl-3-pyrazolin-5-one www.nature.com/scientificreports/ligand has been finely tuned to create a versatile ligand system that can be formed through condensation with a variety of reagents, such as aldehydes, ketones, phenols, carbazides, and others [9][10][11][12][13][14] .The coordination of metal ions is often intertwined with and influences the biological properties of Schiff bases [15][16][17] .Cu(II), Ni(II), and Co(II) Schiff base complexes derived from 4,6-diacetylresorcinol have been synthesized and characterized spectroscopically.Furthermore, their cytotoxic activity against MCF-7 cell lines has been investigated 16 .This publication delves into the production of a novel multifunctional ether ligand and its metal complexes as potential anti-breast medicines, as well as the exploration of molecular docking techniques.Molecular docking is a process used to position a ligand molecule on a receptor molecule in order to form a stable compound.This orientation is employed to predict the binding affinity and the strength of the ligand-protein association through a scoring formula.The interaction between the drug and receptor predicts the affinity and activity of the molecule, making it a critical aspect of drug design and discovery.The total free energy of the system is reduced.Discovering and developing new drugs is a challenging endeavor, often aided by the In-Silico technique.Computer-based drug design plays a crucial role in expediting the drug discovery process.It is particularly valuable in the fields of molecular structural biology and computational drug design, allowing for the prediction of the three-dimensional structure of a molecule.Virtual screening is currently performed by ranking candidates docking for large libraries of compounds using the scoring formula 12 .

Materials and methods
All of the chemicals utilized in the preparation of the ligand and its complexes were of synthetic grade and were employed without any additional purification.The purity of the compounds was confirmed through the utilization of Thin Layer Chromatography (TLC).The analyses of Carbon (C), Hydrogen (H), Nitrogen (N), and Chlorine (Cl) were conducted at the Analytical Unit of Cairo University in Egypt, metal ions were determined using a standard gravimetric technique 10,13 .All metal complexes were subjected to vacuum drying using P 4 O 10 .The measurement of the Infrared (IR) spectra of KBr pellets was performed using a Perkin-Elmer 683 spectrophotometer (4000-400 cm −1 ).The electronic spectra of DMF were obtained using a Perkin-Elmer 550 spectrophotometer.The conductance (10 −3 M) of the complexes in DMF was measured at 25 °C utilizing a Bibby conductometer type MCl.The 1 H-NMR spectra of the ligand and its Zn(II) complex were acquired using a Perkin-Elmer R32-90-MHz spectrophotometer with TMS as an internal standard.The mass spectra were captured employing a JEULJMS-AX-500 mass spectrometer equipped with a data system.The thermal studies (DTA and TGA) were conducted in air on a Shimadzu DT-30 thermal analyzer with a heating rate of 10 °C per minute in the range of 27 to 700 °C.The magnetic susceptibilities were measured at 25 °C using the Gouy method and the magnetic susceptibility standard mercuric tetrathiocyanatocobalt(II).The magnetic adjustments were calculated utilizing Pascal's constant.The magnetic moments were computed using a specific equation 10,13 : The Electron Spin Resonance (ESR) spectra of the solid complexes at room temperature were obtained utilizing a Varian E-109 spectrophotometer with 2,2-diphenyl-1-picrylhydrazyl (DPPH) as a reference material.The samples for Transmission Electron Microscopy (TEM) were prepared by depositing colloids onto carbon-coated TEM grids and allowing the liquid carrier to evaporate in air before being examined with a JEOL1230 transmission electron microscope (120 kV).The synthesis of compound (I) and compound (II) [4,4′-(butane-1,4diylbis(oxy))bis(N-(2-aminoethyl)benzamide)] was performed.Compound I, Fig. 1 was synthesized by adding dibromoethane (1.878 ml, 0.01 mol) to methyl-p-hydroxybenzoate sodium salt (2.49 g, 0.02 mol) dissolved in 50 cm 3 of pure ethanol and heating with stirring for 1 h.Compound II (product) was obtained by dissolving two moles of ethylenediamine (1.001 g) in 50 cm 3 of 100% ethanol and subsequently adding it to compound I.The resulting mixture was refluxed on a water bath for an additional hour, then cooled to room temperature, filtered, washed with distilled water, dried, and finally recrystallized from ethanol to yield the pure product.

Biological activity
The evaluation of cytotoxic activity was conducted in the Pathology Laboratory of the Pathology Department at the Faculty of Medicine, El-Menoufia University, Egypt.The ligand and its metal complexes were tested against breast and liver protein cell lines using the Sulfo-Rhodamine-B-stain (SRB) test technique 17,18 .To ensure adhesion to the plate wall, the cells were plated on a 96-multiwell plate (104 cells/well) for 24 h prior to treatment with the compounds.The compounds were administered to the cell monolayer in DMSO (0, 5, 12.5, 25, and 50 g/ml), with triplicate wells created for each dose.The monolayer cells were then incubated with the complexes for 48 h at 37 °C with 5% CO 2 .Following incubation, the cells were fixed, washed, and stained with Sulfo-Rhodamine-B dye.Acetic acid was used to remove excess stain, while Tri EDTA buffer was used to restore attached stain.The intensity of the colors was measured using an ELISA reader.By plotting the relationship between surviving fraction and drug concentration, the survival curve for each tumor cell line after the addition of the specified substance was obtained.Furthermore, molecular docking was performed using specific complexes with breast www.nature.com/scientificreports/cancer protein 3S7S and liver cancer protein 4OO6 13 .We select liver cell one time and breast cell one time to have more variation defects in of proteins and cells.

Results and discussion
All of the complexes are nanoparticles that are stable at room temperature, non-hydroscopic, insoluble in water, and slightly soluble in common organic solvents like CHCl 3 , but soluble in DMF and DMSO.The analytical and physical data of the ligand and its complexes were provided in Table 1, and the spectral data (Tables 2, 3, 4, 5, 6) were compatible with the proposed structures, as shown in Figs. 2 and 3. Table 1 shows that the molar conductance in DMF solution were in the 6.3-16.4Ω −1 cm 2 mol −1 range, indicating a non-electrolytic nature 19 , some complexes high values showed partial dissociation in DMF.Complexes (2)- (11) were formed by reacting (1) with metal salts in ethanol at (1L: 2M) molar ratios.The complexes were composed of diatomic complexes.The comparison of the infrared (IR) spectra of the ligand (1) and its metal complexes (2)- (11) provides insight into the bonding mechanism between the ligand and the metal ion.The presence of two types of hydrogen bonding, both intra-and intermolecular, is indicated by bands observed in the 3560-3350 cm −1 and 3340-2980 cm −1 ranges of the ligand 20 .The higher frequency range corresponds to a weaker hydrogen bond, whereas the lower frequency band is associated with a stronger hydrogen bond.Additionally, a medium band at 3140 cm −1 is attributed to the v(NH) group 21,22 .Interestingly, the v(NH) group in the complexes appears at the same location as in the free ligand, indicating that the NH group does not participate in metal ion coordination 23 .The v(NH) band is discovered in the metal complexes within the 3013-2546 cm −1 range.Furthermore, the v(OH) group is responsible for a strong band observed at 3360 cm −1 , which appears in the complexes within the 3480-3420 cm −1 range.The v(C=O) group causes a strong band at 1690 cm −1 , observed in the complexes within the 1685-1644 cm −1 range.Bands observed within the ranges of 3560-3210 cm −1 and 3370-3820 cm −1 in the complexes are attributed to hydrated, coordinated water molecules and hydrogen bonding.Another strong band at 1625 cm −1 is caused by the v(C=N) band, which occurs throughout the complex between 1601 and 1620 cm −1 .The acetate ion band v(Ac) is observed within the range of 1449-1260 cm −1 , while the sulphate ion band v(Ac) emerges at 1160, 1164, 1040, and 690 cm −1 .Aromatic bands are observed in two regions: the first within the range of 1570-1460 cm −1 and the second within the range of 853-739 cm −1 for the ligand.In the complexes, aromatic bands are observed in two regions as well: the first within the range of 1519-1402 cm −1 and the second within the range of 771-617 cm −1 .Specifically, the band at 405 cm −1 is attributed to v(Cu-Cl) complex (11) 24,25 .Complexes with bands within the range of 676-610 cm −1 are assigned to v(M-O), while bands within the range of 512-510 cm −1 are assigned to v(M-N) 26,27 .The 1 H nuclear magnetic resonance (NMR) spectra of the ligand (1) and its Zn(II) complex (5) reveal significant information.In the ligand's 1 H-NMR spectra, a peak at 7.7 ppm is observed due to an NH group proton, while peaks within the range of 3.5-4.37ppm are attributed to methylene group protons.Protons from the OH aromatic group are detected at 10.8 ppm.Due to the presence of strong hydrogen bonding, the protons of aromatic rings are observed at 6.6-7.3 ppm, and the protons of the methine group appear at 8.8 ppm 28,29 .In the 1 H-NMR spectrum of the complexes (5), NH protons are observed at 7.8 ppm, and methylene protons are observed within the range of 3.56-4.47ppm.Peaks at 10.5 ppm are due to aromatic OH, while aromatic ring protons emerge within the range of 6.4-7.15ppm.Finally, peaks at 8.3 and 9.0 ppm are observed due to methane group protons 30,31 .When comparing the 1 H-NMR of the ligand and the spectra of complex (5), a significant downfield shift of the proton signal in relation to the free ligand is observed, indicating that the metal ions are coordinated to the ligand.This shift could be attributed to the formation of a coordination bond (NM) [32][33][34] .The proposed formulations of the ligand (1), its Ni(II) complexes (3), and Mn(II) complex ( 6) are supported by the mass spectra.The spectrum of the ligand shows molecular ion peaks (m/z) at 622 amu, which is consistent with the molecular weight of the ligand (622).Furthermore, the observed fragments at m/z = 65, 93, 121, 152,  196, 268, 329, 415, 532, and  Thermal analyses including differential thermal analysis (DTA) and thermogravimetric analysis (TGA) were carried out in order to validate the presence of water molecules, as indicated by the IR spectra.The thermal stability of complexes ( 2), ( 6), (9), and (11) was confirmed within the temperature range of 27-700 °C, with no significant changes observed up to 45 °C.The disruption of hydrogen bonds was observed as an endothermic peak at temperatures ranging from 45 to 55 °C, as shown in Table 4. Subsequently, endothermic peaks were detected within the temperature range of 80-120 °C, corresponding to the loss of hydrated water molecules.Furthermore, the removal of coordinated water molecules occurred in the 140-260 °C range [35][36][37] .The thermogram of the Cu(II) complex (2) exhibited a disintegration process that took place in six phases.The first phase occurred at 55 °C, resulting in a minor weight loss accompanied by an endothermic peak, possibly attributed to the breaking of hydrogen bonds.The second phase occurred at 135 °C with a weight loss of 1.68% (Calculated: 1.70%) and an endothermic peak, which could be associated with the removal of two hydrated water molecules.The elimination of coordinated water molecules took place during the breakdown process at 260 °C, causing a weight loss of 3.85% (Calculated: 3.46%).The removal of four acetate groups generated an endothermic peak at 375 °C with a weight loss of 23.42% (Calculated: 23.50%).The complex displayed an endothermic peak at 540 °C due to its melting point.Finally, exothermic peaks were observed at 415, 490, 680, and 840 °C, indicating a gradual oxidative  30 .Similarly, the thermogram of the Mn(II) complex ( 6) revealed a disintegration process occurring in six stages.The first stage took place at 50 °C, resulting in a minor weight loss accompanied by an endothermic peak, potentially associated with the disruption of hydrogen bonds.The second stage occurred at 120 °C, leading to a weight loss of 4.98% (Calculated: 5.09%) due to the removal of two hydrated water molecules.The breakdown process involved the elimination of coordinated water molecules at 210 °C, causing a weight loss of 1.98% (Calculated: 2.05%).The removal of four acetate groups generated an endothermic peak at 370 °C with a weight loss of 24.29% (Calculated: 24.38%).The complex exhibited an endothermic peak at 580 °C due to its melting point.Finally, exothermic peaks were observed at 410, 465, 650, and 830 °C, indicating a slow oxidative thermal breakdown, resulting in a weight loss of 22.88% (Calculated: 23.33%) and yielding 2MnO 37 .The thermogram of the Mn(II)/Zn(II) combination ( 9) indicated a disintegration process occurring in six phases.The first phase occurred at 48°C, with no weight loss observed, but accompanied by an endothermic peak, potentially attributed to the breaking of hydrogen bonds.
The second phase occurred at 85 °C, resulting in a weight loss of 3.36% (Calculated: 3.42%) and an endothermic peak, which could be related to the removal of two hydrated water molecules.The breakdown stage, caused by the removal of coordinated water molecules, occurred at 140 °C, resulting in a weight loss of 3.50% (Calculated: 3.55%) 37 .The removal of four acetate groups could explain the endothermic peak reported at 280 with 24.02% weight loss (Calc.24.11%).Because of its melting point, the complex displayed an endothermic peak at 380°C.Finally, exothermic peaks arise at 415, 550, 680, and 745 °C, corresponding to oxidative thermal breakdown that progresses slowly, leaving with 20.38% weight loss (Calc 20.47%) of MnO and ZnO 38,39 .According to the thermogram of Cu(II) complex (11) the complexes disintegrated in six phases.The first occurred at 48 °C with no weight loss as an endothermic peak, which could be attributed to hydrogen bonds breaking.The second phase occurred at 85 °C, with a weight loss of 3.38% (Calc.3.74%) as an endothermic peak, which might be attributed to the removal of two hydrated water molecules.The elimination of coordinated water molecules happened during the breakdown process, which occurred at 190 °C with 3.81% weight loss (Calc.3.88%).The removal of four acetate groups resulted in an endothermic peak at 315C o with 15.85% weight loss (Calc.15.92%).Because of its melting point, the complex displayed an endothermic peak at 380 °C.Finally, exothermic peaks developed at 439, 460, and 580 °C, corresponding to gradual oxidative thermal breakdown, leaving 2CuO with a weight loss of 21.15% (Calc.21.21%) [40][41][42] .The thermal data are present in Table 4.

Electron spin resonance (ESR)
The spectral data for complexes (2), ( 6), ( 8), ( 9), (10), and (11) were provided in Table 6.The spectra of the copper(II) complexes ( 2), (10), and (11) exhibited the characteristic of a d 9 configuration, with a ground state of d(x 2 − y 2 ), which is commonly observed in copper (II) complexes.The complexes displayed g|| > g┴ > 2.0023, indicating an octahedral geometry around the copper(II) ion 50,51 .The g-values can be described by the equation G = (g||− 2)/ (g┴ − 2) 50,52 , where G represents the exchange coupling interaction parameter.If G < 4.0, it signifies the presence of a significant exchange coupling, while a G value > 4.0 suggests that the local tetragonal axes are aligned parallel or slightly misaligned.Complexes (2), (10), and ( 11) exhibited values of 3.0 and 2.83, indicating spin-exchange interactions between Cu(II) ions.This phenomenon is further confirmed by the values of the www.nature.com/scientificreports/magnetic moments (see Table 5).The g||/A|| value is also considered as a diagnostic term for stereochemistry 53 .The values of g||/A|| were 210 and 175.8, which are expected for distorted octahedral complexes.The g-values of the copper(II) complexes with a 2B1g ground state (g|| > g┴) can be expressed as K112 54 . (1)

by
The calculated dipolar coupling for unit occupancy of the d orbital is denoted as A° and 2B°, respectively.During the data analysis, all sign combinations of the Cu hyperfine coupling components were taken into account.Physically meaningful results are only obtained when A|| and A^ are negative.The resulting isotropic coupling constant is also negative, as well as the parallel component of the dipolar coupling 2B (− 175.7 and − 177.2 G).These outcomes can only be observed for an orbital involving the (dx 2 − y 2 ) atomic orbital on copper.The value for 2B is within the normal range for copper(II) complexes.The |Aiso| value is relatively small.By dividing the 2B value by 2B° (the calculated dipolar coupling for unit occupancy of d(x 2 − y 2 ) (235.11 G), using Eq. ( 10), it is suggested that all orbital populations are 74.75% and 94.41% d-orbital spin density, indicating the presence of the dx 2 − y 2 orbital for the unpaired electron 47 .On the other hand, the Co(II) complex (4), Mn(II) complexes ( 6) and ( 9), and [Cu(II)/Zn (II)] complex (8), as well as the Cu(II) complex (10), exhibit isotropic values of 2.02, 2.03, and 2.13, respectively, indicating an octahedral structure with covalent bond character 35.

Transmission electron microscopic characterization (TEM)
The average diameter of the complexes particles under investigation was determined to be in the 13.0-36.4nm range, as shown in Figs. 4, 5, and 6.The particles range in size from 1 to 100 nm.The complexes exhibited improved size dependent characteristics.Increased active agent surface area results in faster dissolution of the active agent in an aqueous environment such as the human body, faster dissolution, with gore 61 .The TEM images for Cu(II) complex (2), Ag(I) complex (7), and Cu(II)/Zn complex (8) were showed in Figs. 4,   Chemotherapeutic investigations: The data presented in Figs. 7, 8 and 13) depict the biological efficacy of ligand (1) and its metal complexes (2), (3), ( 5), (6), and (7) against HEPG-2 cell lines.In this study, we aim to compare the chemotherapeutic potential of the evaluated complexes with the reference medication as shown in Fig. 7, Cisplatin.Altering the anion, coordination sites, and metal ion type seems to impact the biological behavior by affecting the ability to bind DNA 51,52,62 .Gaetke and Chow previously proposed that metals may induce oxidative tissue damage through a free-radical mediated pathway similar to the Fenton reaction.It has been demonstrated that Cu(I) complexes are capable of forming GSSG (Glutathione disulfide) through the following reactions [47][48][49] :   The DNA-bound Cu(II) complex undergoes oxidation in the presence of H 2 O 2 , potentially generating Cu(II) (oxo/hydroxo) species [54][55][56][57] .As a consequence, the interaction between the Cu(II) complex and nucleic acid occurs through the Cu-oxo/hydroxy intermediate.Cu(II) complexes are well-known for their redox activity, which seems to be involved in biological processes [58][59][60] .The conversion of Cu(II) to Cu(I) by intracellular thiols, such as GSH (glutathione, a nonenzymatic antioxidant in environments containing oxygen), forms the basis for the redox cycling of Cu complexes 61 .Equations (13-15) illustrate the chemical pathway for [Cu(II) (ligand)] complexes.To summarize, Cu (II) complexes readily combine with GSH 42 , resulting in the formation of Cu(I) complexes and GS+.This Cu(I) complex can generate a superoxide anion in the presence of oxygen, which can induce ROS through a fenton-like reaction:  www.nature.com/scientificreports/As previously described 51 , treatment with the different complexes in DMSO had an equivalent impact on the utilized tumoral cell line.The solvent dimethyl sulphoxide (DMSO) did not influence cell growth.The ligand (1) exhibited a mild inhibitory effect at the tested concentrations, while the complexes had a more significant effect on HEPG-2 cell lines.The ratio of surviving fraction against MCF-7 tumor cells increased as the concentration decreased within the examined concentration range 50 .The cytotoxicity data indicated that the complex (7) under investigation were effective.complex (7) was the most detrimental to cell lines, with a half-maximal inhibitory concentration (IC50) value of 9.5 M, followed by complex (6) with an IC50 value of 55 Complex (7)  exhibited cytotoxicity against cell lines at all doses compared to the standard medication (Cisplatin), which had an IC50 value of 15.3 M.This can be attributed to the Cu(II) ion being bound to DNA.Since altering the anion and type of metal ion can impact biological behavior due to differences in DNA binding ability, the evaluation of different complexes is particularly intriguing from this perspective.Tweedy's chelation theory suggests that the chemotherapeutic activity of the complexes can be traced back to the central metal atom 48 .Additionally, the positive charge of the metal raises the acidity of the coordinated ligand containing protons, resulting in stronger hydrogen bonds that enhance biological activity 39 .The cytotoxic effect of the ligand and some of its metal complexes on HEPG-2 cell lines is presented in Table 7, Fig. 8.
Chemotherapeutic investigations: The data presented in Figs. 9, 10, 11, 12 depict the biological efficiency of ligand (1) and its metal complexes (2), (7), and (8) against MCF-7 cell lines.In this study, we aim to compare the chemotherapeutic potential of the evaluated complexes with the reference medication, Cisplatin.Altering the (15)  Cu(I) ligand + O 2 → O 2 + Cu(II) ligand Table 7. Cytotoxic effect of the ligand and some of its complexes against HEPG-2 cell line.
The DNA-bound Cu(II) complex undergoes oxidation in the presence of H 2 O 2 , potentially generating Cu(II) (oxo/ hydroxo) species [54][55][56][57] .As a consequence, the interaction between the Cu(II) complex and nucleic acid occurs through the Cu-oxo/hydroxy intermediate.Cu(II) complexes are well-known for their redox activity, which seems to be involved in biological processes [58][59][60] .The conversion of Cu(II) to Cu(I) by intracellular thiols, such as GSH (glutathione, a nonenzymatic antioxidant in environments containing oxygen), forms the basis for the redox cycling of Cu complexes 61 , as shown before.Treatment with the different complexes in DMSO had an equivalent impact on the utilized tumoral cell line.The solvent dimethyl sulphoxide (DMSO) did not influence cell growth.The ligand (2) exhibited a mild inhibitory effect at the tested concentrations, while the complexes had a more significant effect on MCF-7 cell lines.The ratio of surviving fraction against MCF-7 tumor cells increased as the concentration decreased within the examined concentration range 50 .The cytotoxicity data indicated that the complexes ( 2) and ( 8) under investigation were effective, copper(II) complex (2) was the most detrimental to cell lines, with an IC50 value of 5.0 M, followed by complex (7) with an IC50 value of 2.8, complex (7) exhibited cytotoxicity against cell lines at all doses compared to the standard medication (Cisplatin), Fig. 13    lines.This can be attributed to the Cu(II) ion being bound to DNA.Since altering the anion and type of metal ion can impact biological behavior due to differences in DNA binding ability, the evaluation of different complexes is particularly intriguing from this perspective.Tweedy's chelation theory suggests that the chemotherapeutic activity of the complexes can be traced back to the central metal atom 48 .Additionally, the positive charge of the metal raises the acidity of the coordinated ligand containing protons, resulting in stronger hydrogen bonds that enhance biological activity 39 .The cytotoxic effect of the ligand and some of its metal complexes on MCF-7 cell lines is presented in Table 8, Figs. 9 and 10.

Molecular docking of some complexes with breast cancer protein 3S7S
Molecular modeling, docking is a method which predicts the preferred orientation of one molecule to a second when a ligand and a target are bound to each other to form a stable complex.Knowledge of the preferred orientation in turn may be used to predict the strength of association or binding affinity between two molecules using, for example, scoring functions.The protein 3S7S, which has been selected, serves as a representation of the crystal structure of the chemical that is considered to be physiologically active in the human placenta.Through the utilization of this approach, one is able to ascertain the site at which the ligand-receptor interaction occurs, as well as the type of interaction that takes place.Furthermore, this method also enables the calculation of the distance between the ligand and the receptor within the interaction grid.The scoring energy obtained from the docking computations corresponds to the inhibitory effect exhibited by the relevant ligand.Within the context of the present investigation, the protein 3S7S mirrors the crystal structure of the human placental aromatase enzyme, which is responsible for the synthesis of the estrogen hormone and contributes to estrogen-dependent breast cancer50.Based on the scoring energy, it can be observed that all ligands possess a considerable number of interactions with the receptor protein.The outcomes of the study serve to demonstrate the capacity of the ligand to impede the protein 3S7S.Based on the scoring energy, it can be observed that all ligands possess a considerable number of interactions with the receptor protein.The outcomes of the study serve to demonstrate the capacity of the ligand to impede the protein 3S7S.

Complex 2
In the case of the docked complex 2, it can generally be observed that the distances associated with the effective ligand-receptor interactions, as depicted docked and 2D structure in Fig. 14 in complex (2) are approximately 3.5 A. This indicates the presence of typical actual bonds, thus signifying a significant binding affinity.The nearest interaction, for instance, is identified through H-donors with 3S7S, with a distance of 2.18 A, while complex2 exhibits a scoring energy (S) of 182,278 kcal.Additionally, it was determined that complex2 possesses 17 binding sites comprising various amino acids (Gln 225, lIe 474, His 480, Asp 222, Phe 221, Pro 308, Gln 218, Asp 309, and Arg 192), suggesting their considerable inhibitory effect.Table 8.Cytotoxic effect of the ligand and some of its complexes against MCF-7 cell line., and His 480) with complex(3) were found, suggesting their higher inhibition than copper complex (2).

Complex 5
However, in most cases, the docked and 2D structure of complex (5), Fig. 16 have effective ligand-receptor interaction distances of 3.5 A, indicating the presence of typical actual bonds and thus strong binding affinity.The nearest interaction, for example, is observed via H-donors with 3S7S (2.46 A) and complex (5) With scoring energy (S) 233,009 kcal.Furthermore, seventeen binding sites for various amino acids (Gln 218, Asp 309, Arg 192, Asp 222, and Gln 225) with complex (5) were discovered.

Complex 6
In most cases, the docked and 2D structure of complex (6) in Fig. 17, ligand-receptor interaction distances were 3.5 A, showing the presence of typical real bonds and consequently considerable binding affinity.For example, the nearest contact is found using H-donors with 3S7S (3.28 A) and complex (6) with scoring energy (S) 230,220 kcal.Furthermore, with compound (6), which is regarded to be the most effective complex towards 3S7S protein, seventeen binding sites of different amino acids (Gln 685, Pro 681, Val 724, Phe 721, and Ser 723) were found.

Complex 7
However, the docked and 2D structure of complex (7) in Fig. 18 have effective ligand-receptor interaction distances were ≤ 3.57A in most cases, which indicates the presence of typical real bonds and hence high binding affinity.For example, the nearest interaction is observed via H-donors with 3575 (3.35 A) and complex (7) with scoring energy (S) 173,468 kcal Furthermore, seventeen binding sites were observed of different amino acids (Gln 685, Glu 682, Phe 584, Val 643 and Asp 639) with complex (7).
Hence we can conclude that the inhibition of complex5 > complex 6 > complex 3 > complex 2 > complex 7 to breast cancer protein 3S7S.

Molecular docking of some complexes with liver cancer protein 4OO6
4OO6 represents Crystal structure of human KAP-beta2 bound to the NLS of HCC1 (Hepato Cellular Carcinoma protein 1) This approach elucidates the ligand-receptor site and type of interactions.It also gives an estimation of the distance between the ligand and the receptor inside the interaction grid.The scoring energy of each pose simulated by the docking calculations reflects the degree of inhibition effect of the corresponding ligand.In the present study.

Complex 8
In most cases, the docked and 2D structure of complex (8), Fig. 19 had effective ligand-receptor interaction distances of 3.1 A, indicating the presence of typical actual bonds and thus strong binding affinity.The nearest interaction, for example, is observed via H-donors with 4OO6 (2.17 A) and (complex (8) With scoring energy (S) 244,215 kcal.Furthermore, twelve binding sites of distinct amino acids (Gln 685, lle 722, lle 642, Glu 682, and Ser 723) with complex (8) were found, suggesting their strong inhibition.For example, the nearest interaction is observed via H-donors with 4OO6 (2.17
Hence we can conclude that the inhibition of complex8 > complex 9 > complex 10 to Hepato Cellular Carcinoma protein.

Conclusion
New metal complexes were created in this investigation.The ligand adopted a hex dentate ligand design, while the metal complexes adopted a tetragonal distorted octahedral geometry around metal ions, according to structural and spectroscopic features.Molar conductance studies indicate that all of the complexes are non-electrolytic in nature.The ligand is coupled to the center metal ion, resulting in six-membered rings containing metal ions.The anticancer actions of the ligand as well as some of its metal complexes were evaluated.The toxicity of both the ligand and the metal complexes was shown to be concentration dependent, with increasing complex concentration decreasing cell viability.

Figure 7 .
Figure 7. Evaluation of cytotoxicity of metal complexes against human HepG-2 cell line.

Figure 8 .
Figure 8. IC50 values of the ligand, and some of its metal complexes against human HEPG-2 cell lines.

Figure 9 .Figure 10 .
Figure 9. Evaluation of cytotoxicity of metal complexes against human MCF-7 cell line.
, which had an IC50 value of 15.3 M. The IC50 value is 15.3 M Complex (8), Figs. 8 and 9 also displayed cytotoxicity against cell

Figure 14 .
Figure 14.(a) Virtual molecular docking of the best docked complex (2) with 3S7S protein and (b) 2D structure of molecular docking of complex(2) with 3S7S protein.

Figure 15 .
Figure 15.(a) Virtual molecular docking of the best docked complex (3) with 3S7S protein and (b) 2D structure of molecular docking of complex (3) with 3S7S protein.

Figure 16 .
Figure 16.(a) Virtual molecular docking of the best docked complex (5) with 3S7S protein and (b) 2D structure of molecular docking of complex (5) with 3S7S protein.

Figure 17 .
Figure 17.(a) Virtual Molecular docking of the best docked complex (6) with 3S7S protein and (b) 2D structure of molecular docking of complex(6) with 3S7S protein.

Figure 18 .
Figure 18.(a) Virtual Molecular docking of the best docked complex (7) with 3S7S protein and (b) 2D structure of molecular docking of complex (7) with 3S7S protein.

Figure 19 .
Figure 19.(a) Virtual Molecular docking of the best docked complex (8) with 4OO6 protein and (b) 2D structure of molecular docking of complex (8) with 4OO6 proteins.

Figure 20 .
Figure 20.(a) Virtual molecular docking of the best docked complex (9) with 4OO6 protein and (b) 2D structure of molecular docking of complex (9) with 4OO6 protein.

Figure 21 .
Figure 21.(a) Virtual Molecular docking of the best docked complex (10) with 4OO6 protein and (b) 2D structure of molecular docking of complex (10) with 4OO6 protein.

Table 2 .
IR frequencies of the bands (cm −1 ) of ligand [H 4 L 2 ] and its metal complexes and their assignments.

Table 4 .
Thermal data for some metal complexes.

Table 5 .
The