Expression of a recombinant endolysin from bacteriophage CAP 10-3 with lytic activity against Cutibacterium acnes

The bacteriophage CAP 10-3 forming plaques against Cutibacterium acnes which causes skin acne was previously isolated from human skin acne lesion. Incomplete whole genome sequence (WGS) of the bacteriophage CAP 10-3 was obtained and it had 29,643 bp long nucleotide with 53.86% GC content. The sequence was similar to C. acnes phage PAP 1-1 with a nucleotide sequence identity of 89.63% and the bacteriophage belonged to Pahexavirus. Bioinformatic analysis of the WGS predicted 147 ORFs and functions of 40 CDSs were identified. The predicted endolysin gene of bacteriophage CAP 10-3 was 858 bp long which was deduced as 285 amino acids (~ 31 kDa). The protein had the highest similarity with amino acid sequence of the endolysin from Propionibacterium phage PHL071N05 with 97.20% identity. The CAP 10-3 endolysin gene was amplified by PCR with primer pairs based on the gene sequence, cloned into an expression vector pET-15b and transformed into Escherichia coli BL21(DE3) strain. The predicted protein band (~ 33 kDa) for the recombinant endolysin was detected in an SDS-PAGE gel and western blot assay. The concentrated supernatant of cell lysate from E. coli BL21(DE3) (pET-15b_CAP10-3 end) and a partially purified recombinant CAP 10-3 endolysin showed antibacterial activity against C. acnes KCTC 3314 in a dose-dependent manner. In conclusion, the recombinant CAP 10-3 endolysin was successfully produced in E. coli strain and it can be considered as a therapeutic agent candidate for treatment of human skin acne.

Cutibacterium acnes is a Gram-positive aerotolerant anaerobic bacterium.C. acnes is mainly found in the areas of the skin rich in sebaceous glands; it accounts for about 90% of the total skin microbiota making it the most abundant bacterium 1,2 .Its involvement in several disorders like eye infections, prostate cancer, acne vulgaris 3 sarcoidosis 4 and rarely bacterial endocarditis 5 has caused its status shifted from commensal bacterium to an opportunistic pathogen 6 .Acne vulgaris is the most abundant skin disorder mainly effecting adolescents.Besides being a skin disorder, it can prove to be an emotional, psychological and social challenge causing depression 7,8 .C. acnes is believed to be the main contributor in the pathology of acne vulgaris where it leads to the formation of acne lesions by eliciting inflammatory response.It's conventional treatment with topical and systematic antibiotic administration has led to an upsurge in resistance and detection of antibiotic resistant C. acnes strains has become increasingly common [9][10][11][12] necessitating search of new non-conventional alternatives among which, phage therapy can be a promising option.
Although phage therapy as a potential antibacterial treatment has been around since pre-antibiotic era (since almost a decade before the first antibiotic was discovered), discovery and widespread successful use of antibiotics caused phages' therapeutic use limited to the Soviet Union 13 .For many years, poorly understood phage biology, lack of quality control and inconsistent and unreliable trials caused the phage therapy to lose attention and phage use was predominantly limited for the research purpose 14 .
However, ever-rising problem of antibiotic resistance has made them a potential candidate to replace or augment the antibiotic treatment 15 .Renewed research interest in the phage therapy in last two decades has come up with its several promising advantages that include host specificity, exponential rate of replication, reproducibility 16 , quick elimination from the body in the absence of host 17 , reduced toxicity to humans, ability OPEN 1 Major of Biotechnology, Korea National University of Transportation, Jeungpyeong 27909, Korea. 2 Major in IT•Biohealth Convergence, Department of IT•Energy Convergence, Graduate School, Korea National University of Transportation, Chungju 27469, Korea. 3These authors contributed equally: Ja-I Kim and Muhammad Adeel Hasnain.* email: gsmoon@ut.ac.kr www.nature.com/scientificreports/ to destroy biofilms 18 and disrupt vegetative cells 19 .Recent research suggests that phages can be used to increase the efficacy of the antibiotics 16 and in some cases revert the antimicrobial resistance 20 .
Since using the whole phage poses numerous challenges yet to be addressed like immune response, inactivation by sterilization etc., one possible alternative is to use phage lytic enzymes only 14 .Endolysin is a phage protein capable of lysing target bacteria and has received a considerable attention as a potential antimicrobial agent.One of their recently discovered capability is to cure hard-to-treat infections by crossing cell membranes of epithelial cells 21 .
Since 2001, a number of successful pre-clinical trials have been conducted using lysins to inhibit mucosal colonization of pathogenic bacteria which shows their potential to at-least complement the conventional antibiotic treatment strategies [22][23][24][25][26] .In the current study, whole genome sequencing of previously isolated C. acnes phage CAP 10-3 was carried out followed by its characterization.A total of 147 ORFs were predicted, most of whose proteins showed homology with the already characterized C. acnes bacteriophage PAP 1-1 (GenBank accession no., OP491959).The predicted endolysin gene was successfully amplified, cloned and expressed in E. coli.The recombinant protein demonstrated effective lytic activity against C. acnes KCTC 3314 strain.

Bacterial strains and culture conditions
The bacteriophage CAP 10-3 forming plaques against C. acnes was previously isolated from human skin acne lesions.C. acnes KCTC 3314 strain which was purchased from the Korean Collection for Type Cultures (KCTC) was used in this study.The strain was cultured in Reinforced Clostridial Medium (RCM) (BD, Sparks, MD, USA) in an anaerobic chamber (DG250; Don Whitley Scientific Ltd., Bingley, UK).E. coli BL21 (DE3) was purchased from Enzynomics Co. Ltd. (Daejeon, Korea) and used for protein expression.The strain was grown in Luria-Bertani (LB) broth (BD) and incubated at 37 °C.

Genomic characterization of bacteriophage CAP 10-3
A phage DNA isolation kit (Norgen Biotek Co., Thorold, Canada) was used to isolate phage CAP 10-3 genomic DNA.The isolated genomic DNA was used for whole genome sequencing on iSeq 100 sequencer (Illumina, Inc., San Diego, CA, USA).A library was created for sequencing and the sequences were assembled using the local run manager assembly program (Ver.1.0.0).The library was prepared using an Illumina prep kit using BLT (bead-linked transposomes) method according to the manufacturer's guide (Illumina).Briefly, BLT was used to fragment and tag the DNA with adapter sequence followed by the post tagmentation clean-up using TSB (tagment stop buffer) and TWB (tagment wash buffer).Tagmented DNA was then amplified using limited-cycle PCR (5 cycles) where index adapters and sequences required to generate sequencing cluster were also added.The prepared library was subsequently purified using SPB (sample purification beads) and used for sequencing.Subsequently, protein coding sequences (CDS) were identified by using PHASTER (https:// phast er.ca).NCBI BLAST (https:// blast.ncbi.nlm.nih.gov/ Blast.cgi) and Uniprot (https:// www.unipr ot.org) were used to confirm gene homology and function.Molecular weight and isoelectric point of a putative endolysin were confirmed using ExPASy (https:// www.expasy.org).The whole genome sequence of bacteriophage CAP 10-3 was deposited to the GenBank (accession no., OR039357).

Construction of E. coli expression vector
The genomic DNA of CAP 10-3 was used as the template for PCR amplification of the endolysin gene.The primers CAP10-3_endolysin_1F(A)_NdeI (5ʹ-GGT GGT CAT ATG AGG TTT ATTCC-3ʹ; NdeI recognition sequence is underlined) and CAP10-3_endolysin_R (5ʹ-TCA CTT TTT CAA ACC GTT GAC-3ʹ) were designed and used for the amplification with a PCR premix kit (i-pfu, Intron Biotechnology, Inc., Seongnam, Korea).PCR running conditions were as follows: 95 °C, 5 min for pre-denaturation; 30 cycles of 95 °C-30 s, 55 °C-30 s, and 72 °C-1 min; 72 °C, 5 min for final extension.The PCR products digested with NdeI restriction endonuclease (Beams Biotechnology Co., Ltd., Seongnam, Korea) were ligated with pET-15b expression vector (Novagen, Massachusetts, USA) which was sequentially treated with BamHI restriction endonuclease (Beams Biotechnology), Klenow fragment (New England Biolabs, Boston, MA, USA), and NdeI endonuclease (Beams Biotechnology) by T4 DNA ligase (Bioneer Co., Daejeon, Korea).The recombinant DNA was introduced to the competent E. coli BL21(DE3) by heat shock method according to the manufacturer's instructions.Transformants were selected on LB agar plate supplemented with 100 μg/mL of ampicillin and confirmed by PCR and restriction profile.

Expression of recombinant endolysin in E. coli
E. coli BL21(DE3) transformed with pET-15b_CAP10-3 end for expression of the endolysin gene of bacteriophage CAP 10-3 was inoculated in 100 mL of LB broth containing 100 μg/mL of ampicillin and incubated at 37 °C with 250 rpm to 0.6 of OD 600.For induction of endolysin gene expression, 1 mM of isopropyl-β-dthiogalactopyranoside (IPTG) was added to the culture and incubated at 37 °C with 150 rpm for 4 h (a control culture without the induction was also prepared).The cultures were centrifuged at 5000g for 7 min and the harvested cell pellets and cell lysates were analyzed by SDS-PAGE and Western blot assay to confirm whether the recombinant endolysin was properly expressed.Briefly, the cell pellet was diluted with 6 × Laemmli sample buffer, an appropriate volume of the suspension was boiled at 95 °C, and the sample was subjected to SDS-PAGE under the condition of 110 V, 80 min.The cell pellet was also suspended in 4 mL of Lysis buffer (50 mM NaH 2 PO 4 , 300 mM NaCl, 10 mM imidazole, pH 8.0) and disrupted on ice with a sonicator (Qsonica, Newtown, CT, USA) by 60% amplitude of 6 cycles of 10 s pulse and 10 s interval.The sonicated sample was centrifuged at 10,000g for 20 min at 4 °C, the supernatant was filtered with a syringe filter (0.45 μm; Anylab, Seoul, Korea), and the filtrate was concentrated by Amicon® Ultra-15 Centrifugal Filter Unit (MWCO.10 K; Millipore, Massachusetts, USA).The concentrate was analyzed by Western blot assay.For the assay, proteins on the SDS-PAGE gel were transferred to Immobilon®-P PVDF membrane (0.45 μm; Merck, Middlesex, USA) and 6 × His-tag recombinant rabbit monoclonal antibody (Invitrogen, Middlesex, USA) and goat anti-rabbit lgG (H + L) secondary antibody with HRP (Invitrogen) were used for detection of His-tagged endolysin in the membrane.

Purification of recombinant endolysin
His-tagged recombinant endolysin was purified by using Ni-NTA agarose (QIAGEN, Hilden, Germany) according to the manufacturer's instruction.One milliliter of Ni-NTA slurry was transferred to a 15 mL tube, centrifuged at 2500g for 5 min, and the supernatant was removed.Two milliliters of lysis buffer was added to the Ni-NTA resin, inverted 10 times, centrifuged, and the supernatant was removed.The resin was then mixed with 4 mL of cell lysate containing recombinant endolysin.The mixture was reacted by shaking at 4 °C for 1 h and transferred to a column (Bio-Rad) with a bottom cap and the cap was removed to collect the flow-through.The resin was washed twice with 4 mL wash buffer (50 mM NaH 2 PO 4 , 300 mM NaCl, 20 mM imidazole, pH 8.0) and eluted 4 times with 0.5 mL elution buffer (50 mM NaH 2 PO 4 , 300 mM NaCl, 250 mM imidazole, pH 8.0).The eluent was concentrated by using an Amicon® Ultra-15 Centrifugal Filter Unit (MWCO.10 K; Millipore) and protein quantification was done by BCA protein assay kit (Thermo Fisher Scientific, Massachusetts, USA).

Evaluation of antibacterial activity of recombinant endolysin
The antibacterial activity of the recombinant endolysin against C. acnes KCTC 3314 strain was evaluated by using the turbidity reduction method with minor modification of the original method by Yoong et al. 27 .The mid-log phase of C. acnes KCTC 3314 strain (1.8 mL) was centrifuged at 10,000g for 5 min and the harvested cell pellet was rapidly frozen in liquid nitrogen and stored at − 20 °C until use or used immediately by suspending in 1.3 mL of 1 × PBS on ice.The cell suspension (270 μL) and 30 μL of the recombinant E. coli cell lysate or purified recombinant endolysin were incubated at 37 °C for 2 h.At every 10 min, optical densities at 600 nm were measured by a microplate reader (Synergy HTX Multi-Mode Reader, BioTek, Vermont, USA).Additionally the viable cell counts were measured by tenfold dilution method at times (0, 2, 4, 6, 8 h) in a case.

Statistical analysis
The experiments in this study were repeated 3 times and expressed as mean ± standard deviation (SD) and statistical analyses were done by using SPSS ver. 25 (Statistical Package for Social Sciences, SPSS Inc., Chicago, IL, USA).The significance was verified by one-way ANOVA, and Duncan's multiple range test (p < 0.05) for absorbance and Scheffe test (p < 0.05) for viable cell count were used for post-hoc.

Ethical approval
The study was performed in agreement with the Declaration of Helsinki principles and approved by the Korea National University of Transportation-Institutional Review Board (KNUT-IRB2020-19).

Bacteriophage CAP 10-3 whole genome sequence
The genome of CAP 10-3 consisted of dsDNA of 29,643 bp with a GC content of 53.86% and 89.63% nucleotide sequence similarity to C. acnes phage PAP 1-1 (GenBank accession no., OP491959), a species of Pahexavirus recently characterized by our group 28 .Homology search using BLASTn showed that it has high degree of similarity to many other C. acnes phages as well.A total of 147 ORFs (open reading frames) were predicted, while the functions of 40 out of 42 coding sequences (CDSs) were identified using PHASTER (Fig. 1).Putative holin and endolysin of CAP 10-3 were very similar to those of C. acnes phage PAP 1-1 with more than 90% amino acid sequence identity.In addition, other proteins like transcriptional regulators, primase, portal protein, exonuclease, capsid, scaffold protein, and sigma factor also showed a wide range of sequence similarity with those of the phage PAP 1-1.TMP (Tape measure protein), in particular, showed a high degree of homology with those of the previously characterized C. acnes phages.The predicted molecular weight of CAP 10-3 endolysin was approximately 31 kDa and the protein had the highest similarity with amino acid sequences of endolysins from Propionibacterium phage PHL071N05 (GenBank accession no., YP_008531700.1)and Cutibacterium phage P108C (GenBank accession no., QHB37111.1)with 97.20% and 95.45% identity, respectively.It also showed similarities with those of N-acetylmuramoyl-L-alanine amidase from Caudoviricetes sp.(GenBank accession no., DAW17174.1)and peptidoglycan recognition family protein from C. acnes (GenBank accession no., WP_303622234.1)with 95.80% and 94.41% identity, respectively.

Construction of recombinant Escherichia coli for expression of endolysin
The putative endolysin gene which was amplified by PCR with primers CAP10-3_endolysin_1F(A)_NdeI and CAP10-3_endolysin_R was confirmed by the size (867 bp) on an electrophoresis gel (data not shown).The sequence includes NdeI restriction endonuclease recognition sequence on 5ʹ region of CAP10-3_ endolysin_1F(A)_NdeI primer and encodes 285 amino acids.In particular, the start codon (GTG) of original sequence was changed into ATG which is more efficient for start of translation in E. coli strains 29 .The recombinant DNA pET-15b_CAP10-3 end was successfully transformed to E. coli BL21(DE3) and it resulted in E. coli BL21(DE3) (pET-15b_CAP 10-3 end).However the band was also detected from the cell pellet without IPTG induction, which means the pET-15b expression system used in this study was not properly controlling the gene expression (Fig. 2).To specifically detect the recombinant His-tagged endolysin, Western blot assay was performed and the specific band for the protein was detected on the transferred membrane.Like the SDS-PAGE result, the band was also detected from the cell lysate without IPTG induction (Fig. 3).

Antibacterial activity of recombinant endolysin against C. acnes
Antibacterial activity of recombinant endolysin against C. acnes was analyzed by using turbidity reduction method.For the beginning, the values among samples were not significantly different but at the middle phase (60 min) the optical densities started to decrease in the recombinant endolysin treatments with dose-dependent manner and at the final phase (120 min) the values were significantly different between treatment of concentrated supernatant of cell lysate for E. coli BL21(DE3) (pET-15b) and that of E. coli BL21(DE3) (pET-15b_CAP10-3 end).
In particular, optical density value of treatment of concentrated supernatant of cell lysate for E. coli BL21(DE3) (pET-15b_CAP10-3 end) was decreased 0.97 to 0.41 and it was also reflected on culture clearance (Fig. 4).In the viable cell count test, the similar trend to optical density test was observed.Briefly, the viable cell count of treatment of concentrated supernatant of cell lysate for E. coli BL21(DE3) (pET-15b) was not significantly different when compared with the starting point during 8 h.However, the cell count in the treatment of concentrated supernatant of cell lysate for E. coli BL21(DE3) (pET-15b_CAP10-3 end) was dramatically decreased with a dose-dependent manner when compared with the starting point during 8 h.In particular, it started 6.15 log CFU/mL of C. acnes and decreased to 4.65 log CFU/mL in the treatment of 560 ug/mL of proteins from concentrated supernatant of cell lysate for E. coli BL21(DE3) (pET-15b_CAP10-3 end) with IPTG induction (Fig. 5).
Based on the results, the recombinant endolysin has antimicrobial activity against C. acnes, which indicates the recombinant strategy is properly working as an active form.The purified recombinant endolysin which includes 6 × His in its N-terminal showed antimicrobial activity against C. acnes in the turbidity reduction test.Namely, the control group using the elution buffer reduced optical density at 600 nm of frozen C. acnes from 0.95 to 0.64, whereas 44, 87, 175 ug/mL of purified recombinant endolysin reduced it from 0.85 to 0.59, 0.92 to 0.45, and 0.94 to 0.35, respectively with a dose-dependent manner (Fig. 6).The culture clearance and microscope images where more cell debris were observed according to increase of concentration of the purified recombinant endolysin also supported the result (Fig. 6).

Discussion
C. acnes is the major contributor in the pathogenesis of acne vulgaris.Increasing antibiotic resistance demands urgent need of exploring new treatment alternatives 8 .Phage therapy has the potential to be an effective and promising antibacterial option in humans as demonstrated by several studies [30][31][32] .In the current study, whole genome sequencing and characterization of C. acnes' phage designated as CAP 10-3 bacteriophage which shows species specificity (Supplementary Table S1) were carried out.CAP 10-3 bacteriophage has a genome of 29.6 kb with a GC content of 53.86%.These findings agree with the previous reports which reported similar range of  GC content and genome size of C. acnes phages [33][34][35][36][37] .CAP 10-3 shares a high degree of similarity (almost 90% nucleotide sequence) with C. acnes phage PAP 1-1.More than 70% of genes with confirmed functions showed a high homology of more than 85% with the CDSs of C. acnes phage PAP 1-1.Additionally, homology search using BLASTn showed that CAP 10-3 also shares a high degree similarity with at least 100 other C. acnes phages.
Particularly, TMP (Tape measure protein used by phages to make channels for delivering their genetic material inside their host cell) showed the highest degree of similarity with the available C. acnes phage genomes.These findings are consistent with the previous report mentioning that genome of C. acnes phages showed more than 85% identity despite their collection from a wide range of geographic region over a 30 year time span 37 and that TMPs are conserved in Siphovirus phages (which infect C. acnes) 3 .Limited genetic diversity, in fact, has been termed as an exception for phages infecting C. acnes 38 since phages infecting other bacteria like Pseudomonas, Staphylococcus, Acinetobacter, Mycobacterium exhibit a wide range of diversity [39][40][41][42] .One potential reason for the lack of diversity in these phages is low genetic diversity in their host strains as described by Tomida et al. 43 which, in turn, is a result of particularly strict nature of the niche in which C. acnes is predominantly found i.e. pilosebaceous unit of human skin 44 .
Despite being specific endolysin have relatively broader range of activity as compared to their parent phage making them a promising antimicrobial agent 45 .Other concerns with phage therapy like possibility of mutation, immune response, the time and cost involved to commercialize their use can also be taken care of by using endolysins 46 .Although commercially available products utilizing endolysin as therapeutic agent are still uncommon, success of one such product Staphefekt and results of several preclinical studies which evaluated its safety and efficacy forecast a promising future for endolysin-based therapy [47][48][49] .Analysis of antibacterial activity of recombinant endolysin showed that it successfully controlled C. acnes KCTC 3314 in a dose dependent manner i.e. higher the concentration of the protein greater was its lytic effect (Figs. 4, 5, 6).This observation has analogy to our recent study in which the phage PAP 1-1 also showed lytic activity against C. acnes in a similar dose dependent way 28 .Varotsou et al. 50have also recently reported dose dependent effect of recombinant endolysin originated from a C. acnes phage against several bacterial species.Our further literature research for use of recombinant endolysin against C. acnes failed to yield any other reports.Most of the available literature is centered on assessing the potential of endolysin against more notorious pathogens such as Staphylococcus, Streptococcus, Pseudomonas spp. 51.Our study, therefore, is one of pioneering reports of using recombinant endolysin against C. acnes.Chandran et al. 52 also reported a dose dependent effect of recombinant endolysin in which viable cell reduction of 1-3.5-log was reported by two different recombinant endolysins, which shows that different endolysins may vary in their lytic ability.Optical density at 600 nm in the turbidity reduction method are also comparable with the mentioned study 53 .Several other studies have also reported dose/ concentration and time dependent cell lysis using endolysins, however with differing results against different bacterial species 45,54,55 which suggests that different lysins may have various abilities of cell lysis depending on their intrinsic characteristics, target host and the medium in which these experiments are conducted.
In conclusion, the recombinant CAP 10-3 endolysin showed antibacterial acivity against C. acnes in a dosedependent manner which indicates that it could be a promising therapeutic candidate to treat skin acne patient.However, further studies on improved solubility of the recombinant endolysin in E. coli strains, improved purification yields, using different bacterial hosts for production, etc. are still necessary for commercialization.

Figure 1 .
Figure 1.The physical map of whole genome sequence of CAP 10-3 bacteriophage.

Figure 6 .
Figure 6.Change of optical density of Cutibacterium acnes KCTC 3314 culture by purified recombinant endolysin produced from Escherichia coli BL21(DE3) (pET-15b_CAP10-3 end).(A) Optical densities during incubation time; (B) Transparency of cell culture in wells; (C) Microscopic images.The experiment was repeated 3 times and expressed as mean ± standard deviation.