Abstract
The global orange industry constantly faces new technical challenges to meet consumer demands for quality fruits. Instead of traditional subjective fruit quality assessment methods, the interest in the horticulture industry has increased in objective, quantitative, and non-destructive assessment methods. Oranges have a thick peel which makes their non-destructive quality assessment challenging. This paper evaluates the potential of short-wave NIR spectroscopy and direct sweetness classification approach for Pakistani cultivars of orange, i.e., Red-Blood, Mosambi, and Succari. The correlation between quality indices, i.e., Brix, titratable acidity (TA), Brix: TA and BrimA (Brix minus acids), sensory assessment of the fruit, and short-wave NIR spectra, is analysed. Mix cultivar oranges are classified as sweet, mixed, and acidic based on short-wave NIR spectra. Short-wave NIR spectral data were obtained using the industry standard F-750 fruit quality meter (310–1100 nm). Reference Brix and TA measurements were taken using standard destructive testing methods. Reference taste labels i.e., sweet, mix, and acidic, were acquired through sensory evaluation of samples. For indirect fruit classification, partial least squares regression models were developed for Brix, TA, Brix: TA, and BrimA estimation with a correlation coefficient of 0.57, 0.73, 0.66, and 0.55, respectively, on independent test data. The ensemble classifier achieved 81.03% accuracy for three classes (sweet, mixed, and acidic) classification on independent test data for direct fruit classification. A good correlation between NIR spectra and sensory assessment is observed as compared to quality indices. A direct classification approach is more suitable for a machine-learning-based orange sweetness classification using NIR spectroscopy than the estimation of quality indices.
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Introduction
Oranges are juicy, refreshing, and Pakistan’s most loved winter fruit. Pakistan is the 6th largest producer of citrus in the world1, and around 0.46 million tons of fruit were exported in the year 20202. Ripeness is very critical as it directly influences the eating quality of harvested fruits3. Oranges are non-climacteric fruits i.e., they don’t ripe further once they are harvested. In Pakistan, quality inspection for fruits to be exported is still carried out subjectively by the packaging industry using sample-based tasting and/or by visualizing physical features, such as fruit color and size. The method is error-prone and tedious. These factors serve as a motivation for the automation of testing procedures. To automate the visual quality inspection, one can utilize camera sensors for estimating size, surface characteristics, and texture4. For gauging taste, sweetness, or other quality measures, one can utilize near-infrared (NIR) spectroscopy-based methods5. The non-destructive assessment using NIR spectroscopy can help to correlate dry matter (DM), Brix, titratable acidity (TA), and colour6 with fruit quality. Such assessment can also help in full batch testing and quality-based segregation as opposed to sample-based manual judgment.
Over the past decades, NIR spectroscopy has gained considerable attention for non-destructive maturity index assessment due to its ease, fast detection speed, and precision7,8. Researchers have used NIR spectroscopy with machine learning regression algorithms to develop maturity index prediction models such as DM, Brix, color, chlorophyll, starch and TA (only in high acid fruit like lemon and mandarin) of various fruits including apple9, pear10, nectarine11, mango12, banana13, melon14, mandarin15, strawberry16, apricot17, kiwifruit18, carambola19, grape20, loquat21 and pineapple22. However, due to the diversity in varieties and growing conditions, it is essential to develop the maturity index prediction model for a particular variety, growing region, and for local or export varieties23. Other applications require direct classification by use of some machine learning classification algorithm rather than quantification of quality parameter levels. For example, nectarine cultivars24,25, orange cultivars26, and orange growing regions27 have been differentiated, maturity classes of durian28, avocado29 and mango fruit30, internal defects detection of mango31, citrus32 and apple33, storage potential classification of kiwi34 and sweetness levels of melon35 and grapes36 have been classified.
Most of the published research on the measurement of intact fruit internal parameters have used wider wavelength regions including extended NIR region (> 1000 nm)7, e.g. for ‘Valencia’ orange 450–2500 nm37, for citrus 1100–2500 nm38, for ‘Satsuma’ mandarin 400–2350 nm39. The short-wave NIR (SWNIR) region (750–1100 nm) is used commercially for the assessment of internal quality attributes of intact fruit, in preference to the extended NIR region7. Longer wavelength ranges offer narrower and stronger absorption features as compared to SWNIR and thus better evaluation of internal parameters however, the SWNIR wavelengths have greater effective penetration depth into the fruit, hence, offer robustness across independent populations and given the variation in outer layer attributes. The short-wave Vis–NIR option is preferred for commercial purposes due to (currently) lower hardware costs7,8. Kim et al.40 reported RMSEP and RP of 0.514 oBrix and 0.80 respectively for ‘Unshiu’ orange Brix prediction PLSR model trained using wavelength range 472-1156 nm. Luo et al.41 for Brix prediction of ‘Navel’ orange built PLSR model using wavelength range 450–1000 nm and reported RMSEP and RP of 1.35 oBrix and 0.80, respectively. It is observed in both of the experiments40,41 that the wavelength region includes visible region as well along with SWNIR region and the results are for a single cultivar dataset. McGlone et al.42 used NIR direct transmission measurement mode with a spectral window of 700–930 nm for Brix and TA prediction of ‘Satsuma’ mandarin. The best results for Brix prediction are R and RMSEP of 0.96, 0.32% and for TA it is stated that accurate TA prediction was not possible.
The pulp of oranges is covered inside a thick peel, which makes penetration of NIR spectroscopy challenging. Since ripening and harvest maturity is the same for non-climacteric fruits, there can be two ways to estimate ripeness/maturity. The first method is to estimate the fruit quality parameters like Brix, TA, etc. using a machine learning regression algorithm and based on their values judge the sample quality as done in15,37,38,39,40,41,42. The second method is to directly classify the eating quality using a machine learning classification algorithm, as reported by researchers in35,36 for the direct sweetness classification of melons and grapes. To the best of author’s knowledge, SWNIR spectroscopy is never investigated for direct sweetness classification of orange fruit. Moreover, the potential of SWNIR spectroscopy and direct sweetness classification for mixed cultivar datasets needs to be analyzed.
Like oranges, melons also have a thick rind. Zeb et al.35 have previously proposed a direct sweetness classifier for melons as opposed to Brix-based thresholding, using the correlation between short-wave NIR spectroscopy and sensory assessment. The proposed direct sweetness classifier tested on a single cultivar of melons i.e., ‘honey’ melons, outperformed the Brix estimation-based indirect classification method35. There is a need to evaluate the correlation of SWNIR spectroscopy and sensory assessment in other fruits as well and mixed cultivar datasets. As an extension of the Zeb et al.35 work, in this paper, the potential of SWNIR spectroscopy and direct sweetness classification through machine learning modelling is evaluated for Pakistani cultivars of orange i.e., Blood red, Mosambi, and Succari (average peel thickness 6 mm). A correlation is developed between quality indices i.e., Brix, TA, Brix: TA, and BrimA (Brix minus acids), the sweetness of the fruit, and NIR spectra which are then classified as sweet, mixed, and acidic using a machine learning classifier based on NIR spectra. We argue that direct classification is more suitable to evaluate orange sweetness as opposed to estimating quality indices.
Materials and methods
Fruit samples
Orange (Citrus sinenses (L.) Osbeck), cultivars (cvs.) ‘Blood red’, ‘Mosambi’ and ‘Succari’) ripened samples were harvested with due permission from orchard (Ghulam Rasool Farms) located in Chakwal district of Punjab province on two dates i.e., the first one started on 10th Jan 2021 and the second one on 28th Jan 2021 (33 of Blood red, 32 of Mosambi and 27 of Succari; 92 fruits in total). Average peel thickness was 6 mm. Sixty-four samples were used for model calibration, with each fruit scanned on two sides for Brix and TA to give 128 spectra. Twenty-eight samples (total 56 spectra) were used for model validation (see Table 1 for details). Samples within each fruit were treated as independent spectral set. All methods related to sample collection, destructive testing and sensory assessment were performed in accordance with the relevant guidelines/regulations/legislation.
Collection of Vis/NIR spectra
Orange samples were marked on-tree on opposite sides i.e. sun facing side and non-sun facing side (180° apart approximately) as shown in Fig. 1, to account for within fruit variations. After marking samples on-tree, the oranges were harvested on two dates (both harvest dates were one week apart) and brought to a local laboratory at National Centre of Robotics and Automation (Islamabad, Pakistan) and stored at room temperature for 24 h to minimize the influence of sample temperature on prediction accuracy43. Three spectra were collected from each position and average was computed. Vis–NIR spectra (range 400–1150 nm) were collected using the F-750 (Felix Instruments, Camas, WA, USA). This device employs interactance optical geometry and a Carl Zeiss MMS-1 spectrometer, with a pixel spacing of approximately 3.3 nm and a spectral resolution (FWHM) of 8–13 nm. It uses a halogen lamp as a light source.
Reference measurements
For reference measurements, the marked region (along with surrounding tissues to get a suitable representation of the core as well) was excised and skin was removed. The extracted flesh was squeezed using a garlic press. Brix was assessed of a sample of the extracted juice using a digital refractometer (Model: PAL-1 [°Brix 0–53%], Atago Co., Ltd, Tokyo, Japan). The refractometer has automatic temperature compensation with range 10–100 °C and measurement accuracy of ± 0.2%.
TA was measured by manual titration of 10 mL of extracted juice with 0.1 M sodium hydroxide (NaOH) using phenolphthalein as an indicator. The acid formula for citrus fruit samples (Eq. 1) was applied to calculate TA, expressed as % citric acid.
Maturity index and BrimA were then calculated by Eqs. (2) and (3) respectively. The value of k in Eq. (3) is taken as 1.
Sensory assessment
Reference values for sweetness were assessed by a briefly trained five judges panel with age between 20 and 50. After spectra acquisition, two slices were cut from the neighbor region from where destructive testing has been performed and presented to two of the judges at random for taste evaluation. Distilled water was provided to judges for drinking after every sample evaluation to clear previous sample taste. Oranges were classified into three classes by sensory evaluation i.e. Sweet, mix (sweet and acidic both) and acidic. The class label of each sample was described by average score of the two judges for that sample. Class wise scoring sheet used for assessment is given in Table 2.
Chemometric analysis
A direct sweetness classification method has been proposed35 by authors for melons sweetness classification as opposed to indirect measure of Brix estimation. As an extension of author’s previous work35, in this paper, we have investigated potential of both the methods for quality assessments of mix cultivar oranges as shown in Fig. 2. The first method exploits the correlation between NIR spectra and fruit quality index parameters to estimate these parameters using machine learning regression algorithm and based on those predicted values, the quality of the sample is classified. The second method exploits the correlation between NIR spectra and sensory assessment to directly classify test sample as sweet, acidic or mix class sample, using machine learning classification algorithm.
Savitzky-Golay (SG) second derivative spectral pre-processing is a famous pre-processing method that usually outperforms other pre-processing methods for spectral data analysis44. Hence, 11-point SG second derivative preprocessing was performed on spectral data. Amongst all regression algorithms, the partial least squares regression is the most widely used regression algorithm for prediction of fruit quality index parameters45. For indirect quality assessment, partial least squares regression was used to build Brix, TA, Brix:TA ratio and BrimA estimation models.
Principle component analysis (PCA) has been widely used with spectroscopic data45 to emphasize variation and bring out strong patterns in the data set. For direct sweetness classification, after pre-processing, PCA was applied on spectral data and then several supervised and unsupervised learning classifiers are implemented and compared including tree, ensemble, K nearest neighbor (KNN), linear discriminant analysis (LDA) and SVM.
For indirect classification, the Unscrambler v11.0 spectral analysis software evaluation version (CAMO PRECESS AS, Oslo, Norway) was used for building combined variety calibration model using calibration dataset (Table 4). 11 points Savitzky-Golay second derivative smoothing filter was applied before building model. The performance of developed models was evaluated by RCV (correlation coefficient of cross validation), RP (correlation coefficient of prediction), RMSECV (root mean square error of cross validation) and RMSEP (root mean square error of prediction). Tenfold cross validation was performed. Prediction models were developed using the Vis/NIR region in the range 600–1050 nm (following41).
For direct classification, MATLAB R 2018a software was used. Input data for both the methods i.e. direct and indirect classification was same (600–1050 nm wavelength values pre-processed with 11-point SG second derivative using Unscrambler software). Classification was performed using MATLAB classification learner module with PCA enabled (first 15 principal components were used).
Results
Dataset statistics
Destructive testing statistics of orange quality index parameters i.e. Brix, TA, maturity index and BrimA with respect to the individual variety are shown in Table 3. The range and mean of Blood red cultivar is relatively low for Brix, Brix:TA ratio and BrimA, and high for TA as compared to other two varieties. Table 3 shows that the statistics of Succari cultivar are dissimilar from the other two investigated cultivars with respect to TA and Brix:TA ratio i.e. TA range (0.14–0.33%) and mean (0.21%) is lowest and maturity index range (33.64–75.63) and mean (55.38) is highest than that of Blood red and Mosambi cultivars.
Since, Succari cultivar is statistically different from the other two cultivars, the models were built using two different combinations of investigated cultivars i.e. dataset-1 contains all three investigated cultivars and dataset-2 contains only Blood red and Mosambi cultivars. Table 4 shows data set wise statistics of quality index parameters.
Figure 3 shows the distribution of quality index values with respect to orange sweetness levels. From 184 samples (92 oranges, 2 samples each), 129 samples belonged to sweet class, 48 belonged to mix class and 7 belonged to acidic class. From Fig. 3, the sweetness levels cannot be concluded based on individual values of Brix, TA, Brix:TA or BrimA, since there is significant overlap between the three sweetness levels and the respective quality indexes. Moreover, it can be concluded that with respect to quality index parameters, Succari cultivar is dissimilar to the other two investigated varieties.
Figure 4 and Table 5 show the statistical correlation amongst the quality indices. Brix and BrimA (Fig. 4a) show a strong positive correlation (R = 0.967) while TA and maturity index (Fig. 4d) show negative correlation (R = − 0.832) for all the three investigated cultivars. Other scatter plots (Fig. 4b,c,e,f) do not show a strong positive/negative correlation amongst the indices for all the investigated cultivars. Table 5 shows that there is no correlation between Brix vs TA and BrimA vs TA for Succari samples.
Overview of spectra
The absorbance spectra of orange fruit (Fig. 5a) is dominated by a peak around 680 nm associated to chlorophyll absorption46. Moreover, broad peaks around 750 nm and 850 nm are observed due to the third overtone of O–H bond stretching and the third and fourth overtones of C-H bond stretching47. Another observed peak at 970 nm is associated with water absorption band (second overtone of O–H bond stretching)48. Second derivative of the spectrum shown in Fig. 5b confirmed all above absorbance peaks. Prediction models were developed using the Vis/NIR region in the range 600–1050 nm (following41) as this is the region of carbohydrates such as glucose, fructose and sucrose47.
Indirect classification results
Table 6 presents the combined variety PLSR model results on Brix, TA, Brix:TA and BrimA with dataset having 184 samples including all three investigated varieties. The cross validation R is 0.69, 0.48, 0.5 and 0.66 respectively and RMSE is 1.00 oBrix, 0.34%, 18.06 and 1.13% respectively.
These models include samples of Succari variety as well, which is a statistically incompatible cultivar (with respect to TA and Brix:TA) with the Blood red and Mosambi cultivars. Hence, Table 7 shows PLSR models trained on Blood red and Mosambi cultivars since they are similar to each other w.r.t TA and Brix:TA statistics. Table 6 shows that excluding Succari samples from dataset and rebuilding PLSR models provided improved results for TA and Brix:TA models. However, Brix and BrimA prediction results were worsened because with respect to Brix, all three investigated varieties have similar statistics. Removing Succari samples reduced the size of data set and hence worse results. Figure 6 shows the scatter plots of predicted vs reference values of the developed PLSR models of Tables 6 and 7.
Direct classification results
To predict orange’s eating quality in terms of sweetness, multi class classification algorithms were implemented on both datasets. The cross validation and prediction result for both data sets are listed in Tables 8 and 9. For dataset1, ensemble classifier achieved 81.03% accuracy for 3 class classification of independent test data. For dataset2, SVM and KNN both achieved 79.49% accuracy for 3 class classification of independent test data.
Observations and discussion
Statistics comparison of investigated cultivars
The “Blood red” variety is the most tasteful (mix to sweet taste) cultivar of orange in Pakistan. Table 3 shows that the range and mean of TA are high and of Brix, Brix: TA, and BrimA are low. Of 66 samples of Blood red, 33 belonged to sweet class, 26 belonged to mix class, and 7 belonged to acidic class.
The Mosambi cultivar is also segregated as sweet by the judges. It can be seen from Table 3 that its range and mean of TA are lesser and for Brix, it’s higher than the Blood red cultivar hence its flavor is generally more sweater than Blood red variety. Amongst 64 samples of Mosambi, 46 belonged to the sweet class and 17 belonged to the mixed class.
The Succari cultivar is a different cultivar in terms of sweetness from the other two cultivars. Succari samples always have a flat sweet taste due to a lack of acid contents. The statistics of quality index parameters also support this claim as its TA range and mean is the lowest and hence Brix: TA values are the highest amongst other investigated. Amongst 54 samples of Succari, 47 belonged to the sweet class and only 5 belonged to the mixed class.
Development of mixed cultivar PLSR models
An attempt was made to predict Brix, TA, Brix: TA, and BrimA using PLSR regression models developed for mixed cultivar datasets. Since the Succari cultivar is statistically (w.r.t TA and Brix: TA) and taste-wise different from the other two investigated cultivars, PLSR models were built for two datasets, one having a mixture of statistically different cultivars i.e. Blood red, Mosambi and Succari and other one having only statistically compatible cultivars i.e. Blood red and Mosambi.
It is observed that since all three investigated cultivars have almost similar Brix and BrimA statistics and are positively correlated (Table 3 and Fig. 4a), hence the model built with data set having all three cultivars achieved better prediction results for Brix and BrimA as compared to the model built with a dataset having only two cultivars i.e. Blood red and Mosambi (Tables 6, 7). This is because dataset 2 has a lesser number of samples than dataset1. The TA and Brix: TA results of PLSR models built with only two cultivars’ data (Blood red and Mosambi) achieved relatively better prediction results than the three cultivar dataset.
Dataset standard deviation (S.D.) is important to determine the value of the NIR spectroscopy technique for fruit quality assessment7. The technique holds significance only when the S.D. of the attribute of interest is greater than the measurement RMSEP. Indeed, the prediction set R is directly related to measurement bias corrected RMSEP and S.D. i.e., for a particular bias corrected RMSEP, higher S.D. will result in a higher RP value7.
For indirect classification, it is observed that the RCV and RP values of the developed PLSR models are low however, the RMSECV and RMSEP are below the S.D. of the datasets (for Brix and BrimA considering S.D. of dataset1 and for TA and Brix: TA considering S.D. of dataset2) (see Tables 3, 4, 5, 6 and 7). The low RP values are because of the low S.D. of the collected dataset, which is a limitation for the presented work as well. Due to the low RCV and RP values, estimation of quality index value using PLSR models is not a suitable option with the investigated dataset, rather the overall sorting using the classification of sweetness levels is a suitable option.
We observed (see Tables 8 and 9) a good correlation between NIR spectra and sensory assessment as opposed to quality indices. Hence, like melons35, direct classification is more suitable for mixed cultivar orange sweetness classification using NIR spectroscopy as opposed to the estimation of quality indices.
Conclusion
The research was carried out to investigate the correlation between quality indices i.e. Brix, titratable acidity (TA), Brix: TA, and BrimA (Brix minus acids), sensory assessment of the fruit, and short wave near-infrared (SWNIR) spectra that were then classified as sweet, mixed, and acidic based on SWNIR spectra for mixed cultivar datasets. Datasets were collected using three Pakistani cultivars of orange i.e., Blood red, Mosambi, and Succari cultivars. It is observed that Succari cultivar is a statistically different cultivar (w.r.t. TA and Brix:TA values) than Blood red and Mosambi cultivars. Hence, two experiments were performed: one with samples of Blood red, Mosambi, and Succari (dataset1), the second with samples of statistically similar cultivars (dataset2) i.e., Blood red and Mosambi. Given both the datasets, the best fit PLSR model for Brix and BrimA is obtained with dataset1 while for TA and Brix:TA, the best fit model is obtained with dataset2. It is concluded that to develop a statistical model, samples of statistically dissimilar cultivars should not be merged to form a single mixed cultivar dataset. Moreover, we observed a good correlation between SWNIR spectra and sensory assessment as opposed to quality indices. Hence, direct classification machine learning model is more suitable for orange sweetness classification using SWNIR spectroscopy as opposed to the developing a machine learning model for estimation of quality indices (Supplementary information S1).
Data availability
The data that support the findings of this study and/or analyzed during the current study available from the corresponding author on reasonable request.
References
Agriculture Statistics, Pakistan Bureau of Statistics” https://www.pbs.gov.pk/content/agriculture-statistics (2022).
Ministry of Finance, Government of Pakistan. https://www.finance.gov.pk/survey_2021.html (2022).
Slaughter, D. C., Nondestructive maturity assessment methods for mango: A review of literature and identification of future research needs (2009).
Cubero, S., Lee, W. S., Aleixos, N., Albert, F. & Blasco, J. Automated systems based on machine vision for inspecting citrus fruits from the field to postharvest—a review. Food Bioprocess Technol. 9(10), 1623–1639. https://doi.org/10.1007/S11947-016-1767-1/TABLES/2 (2016).
Magwaza, L. S. et al. NIR spectroscopy applications for internal and external quality analysis of citrus fruit—a review. Food Bioprocess Technol. 5(2), 425–444. https://doi.org/10.1007/S11947-011-0697-1/TABLES/6 (2012).
Shah, S.S.A. et al., Towards fruit maturity estimation using NIR spectroscopy. Infrared Phys. Technol. 111. https://doi.org/10.1016/J.INFRARED.2020.103479 (2020).
Walsh, K. B., Blasco, J. , Zude-Sasse, M. & Sun, X. Visible-NIR ‘point’ spectroscopy in postharvest fruit and vegetable assessment: The science behind three decades of commercial use. Postharvest Biol. Technol. 168. https://doi.org/10.1016/J.POSTHARVBIO.2020.111246 (2020).
Walsh, K. B., McGlone, V. A. & Han, D. H. The uses of near infra-red spectroscopy in postharvest decision support: A review. Postharvest Biol. Technol. 163. https://doi.org/10.1016/J.POSTHARVBIO.2020.111139 (2020).
Pourdarbani, R. et al. Using metaheuristic algorithms to improve the estimation of acidity in Fuji apples using NIR spectroscopy. Ain Shams Eng. J. 13(6), 101776. https://doi.org/10.1016/J.ASEJ.2022.101776 (2022).
Lu, Z. et al. Nondestructive testing of pear based on Fourier near-infrared spectroscopy. Foods. 11, 1076. https://doi.org/10.3390/FOODS11081076 (2022).
Scalisi, A. & O’Connell, M. G. Application of visible/NIR spectroscopy for the estimation of soluble solids, dry matter and flesh firmness in stone fruits. J. Sci. Food Agric. 101(5), 2100–2107. https://doi.org/10.1002/JSFA.10832 (2021).
dos Santos Neto, J. P., de Assis, M. W. D., Casagrande, I. P., Cunha Júnior, L. C. & de Almeida Teixeira, G. H. Determination of ‘Palmer’ mango maturity indices using portable near infrared (VIS-NIR) spectrometer. Postharvest Biol. Technol. 130, 75–80. https://doi.org/10.1016/J.POSTHARVBIO.2017.03.009 (2017).
Sripaurya, T., Sengchuai, K., Booranawong, A. & Chetpattananondh, K. Gros Michel banana soluble solids content evaluation and maturity classification using a developed portable 6 channel NIR device measurement,. Meas. J. Int. Meas. Confed. 173. https://doi.org/10.1016/J.MEASUREMENT.2020.108615 (2021).
Kim, S. Y., Hong, S. J., Kim, E. C., Lee, C. H. & Kim, G. Neural network based prediction of soluble solids concentration in oriental melon using VIS/NIR spectroscopy. Appl. Eng. Agric. 37(4), 653–663. https://doi.org/10.13031/AEA.14332 (2021).
Huang, C., Cai, J., Zhou, Y., El-Seedi, H. R. & Guo, Z. Fusion models for detection of soluble solids content in mandarin by Vis/NIR transmission spectroscopy combined external factors. Infrared Phys. Technol. 124, 104233. https://doi.org/10.1016/J.INFRARED.2022.104233 (2022).
Saad, A. G., Azam, M. M. & Amer, B. M. A. Quality analysis prediction and discriminating strawberry maturity with a hand-held Vis–NIR spectrometer. Food Anal. Methods. 15(3), 689–699. https://doi.org/10.1007/S12161-021-02166-2/FIGURES/8 (2022).
Buyukcan, M. B. & Kavdir, I. Prediction of some internal quality parameters of apricot using FT-NIR spectroscopy. J. Food Meas. Charact. 11(2), 651–659. https://doi.org/10.1007/S11694-016-9434-9/FIGURES/7 (2017).
Afonso, A. M., Antunes, M. D., Cruz, S., Cavaco, A. M., & Guerra, R. Non-destructive follow-up of ‘Jintao’ kiwifruit ripening through VIS-NIR spectroscopy—individual vs. average calibration model’s predictions. Postharvest Biol. Technol. 188, 111895. https://doi.org/10.1016/J.POSTHARVBIO.2022.111895 (2022).
de Moraes, I. A., Cruz-Tirado, L. J. P. & Barbin, D. F. Online measurement of carambola (Averrhoa carambola L.) physicochemical properties and estimation of maturity stages using a portable NIR spectrometer. Sci. Hortic. (Amsterdam). 304, 111263. https://doi.org/10.1016/J.SCIENTA.2022.111263 (2022).
Basile, T., Marsico, A. D. & Perniola, R. Use of artificial neural networks and NIR spectroscopy for non-destructive grape texture prediction. Foods. 11(3), 281. https://doi.org/10.3390/FOODS11030281 (2022).
Fu, X. P. et al. Determination of soluble solid content and acidity of loquats based on FT-NIR spectroscopy. J. Zhejiang Univ. Sci. B 10(2), 120–125. https://doi.org/10.1631/JZUS.B0820097 (2009).
Amuah, C. L. Y. et al. Feasibility study of the use of handheld NIR spectrometer for simultaneous authentication and quantification of quality parameters in intact pineapple fruits. J. Spectrosc. 2019 (2019). 10.1155/2019/5975461.
Kader, A. A. & Yahia, E. M. Postharvest biology of tropical and subtropical fruits. Postharvest Biol. Technol. Trop. Subtrop. Fruits. 1, 79–111. https://doi.org/10.1533/9780857093622.79 (2011).
Cortés, V., Cubero, S., Aleixos, N., Blasco, J. & Talens, P. Sweet and nonsweet taste discrimination of nectarines using visible and near-infrared spectroscopy. Postharvest Biol. Technol. 133, 113–120. https://doi.org/10.1016/J.POSTHARVBIO.2017.07.015 (2017).
Cortés, V., Blasco, J., Aleixos, N., Cubero, S. & Talens, P. Visible and near-infrared diffuse reflectance spectroscopy for fast qualitative and quantitative assessment of nectarine quality. Food Bioprocess Technol. 10(10), 1755–1766. https://doi.org/10.1007/S11947-017-1943-Y (2017).
Suphamitmongkol, W., Nie, G., Liu, R., Kasemsumran, S. & Shi, Y. An alternative approach for the classification of orange varieties based on near infrared spectroscopy. Comput. Electron. Agric. 91, 87–93. https://doi.org/10.1016/J.COMPAG.2012.11.014 (2013).
Dan, S., Yang, S. X., Tian, F. & Den, L. Classification of orange growing locations based on the near-infrared spectroscopy using data mining. Chang. Publ. TSI Press 22(2), 229–236. https://doi.org/10.1080/10798587.2015.1095474 (2015).
Timkhum, P. & Terdwongworakul, A. Non-destructive classification of durian maturity of ‘Monthong’ cultivar by means of visible spectroscopy of the spine. J. Food Eng. 112(4), 263–267. https://doi.org/10.1016/J.JFOODENG.2012.05.018 (2012).
Melado-Herreros, A. et al. Postharvest ripeness assessment of ‘Hass’ avocado based on development of a new ripening index and Vis-NIR spectroscopy. Postharvest Biol. Technol. 181, 111683. https://doi.org/10.1016/J.POSTHARVBIO.2021.111683 (2021).
Shah, S. S. A. et al. Mango maturity classification instead of maturity index estimation: A new approach towards handheld NIR spectroscopy. Infrared. Phys. Technol. 115, 103639. https://doi.org/10.1016/J.INFRARED.2021.103639 (2021).
Raghavendra, A., Guru, D. S. & Rao, M. K. Mango internal defect detection based on optimal wavelength selection method using NIR spectroscopy. Artif. Intell. Agric. 5, 43–51. https://doi.org/10.1016/J.AIIA.2021.01.005 (2021).
Ghanei Ghooshkhaneh, N., Golzarian, M. R. & Mollazade, K. VIS-NIR spectroscopy for detection of citrus core rot caused by Alternaria alternata. Food Control. 144, 109320. https://doi.org/10.1016/J.FOODCONT.2022.109320 (2023).
Huang, Y., Lu, R. & Chen, K. Detection of internal defect of apples by a multichannel Vis/NIR spectroscopic system. Postharvest Biol. Technol. 161, 111065. https://doi.org/10.1016/J.POSTHARVBIO.2019.111065 (2020).
Li, M., Pullanagari, R., Yule, I. & East, A. Segregation of ‘Hayward’ kiwifruit for storage potential using Vis-NIR spectroscopy. Postharvest Biol. Technol. 189, 111893. https://doi.org/10.1016/J.POSTHARVBIO.2022.111893 (2022).
Zeb, A. et al., Is this melon sweet? A quantitative classification for near-infrared spectroscopy. Infrared Phys. Technol. 114. https://doi.org/10.1016/j.infrared.2021.103645 (2021).
Parpinello, G. P., Nunziatini, G., Rombolà, A. D., Gottardi, F. & Versari, A. Relationship between sensory and NIR spectroscopy in consumer preference of table grape (cv Italia). Postharvest Biol. Technol. 83, 47–53. https://doi.org/10.1016/J.POSTHARVBIO.2013.03.013 (2013).
Ncama, K., Opara, U. L., Tesfay, S. Z., Fawole, O. A. & Magwaza, L. S. Application of Vis/NIR spectroscopy for predicting sweetness and flavour parameters of ‘Valencia’ orange (Citrus sinensis) and ‘Star Ruby’ grapefruit (Citrus x paradisi Macfad). J. Food Eng. 193, 86–94. https://doi.org/10.1016/J.JFOODENG.2016.08.015 (2017).
Tewari, J. C., Dixit, V., Cho, B. K. & Malik, K. A. Determination of origin and sugars of citrus fruits using genetic algorithm, correspondence analysis and partial least square combined with fiber optic NIR spectroscopy. Spectrochim. Acta Part A Mol. Biomol. Spectrosc. 71(3), 1119–1127. https://doi.org/10.1016/J.SAA.2008.03.005 (2008).
Gómez, A. H., He, Y. & Pereira, A. G. Non-destructive measurement of acidity, soluble solids and firmness of Satsuma mandarin using Vis/NIR-spectroscopy techniques. J. Food Eng. 77(2), 313–319. https://doi.org/10.1016/J.JFOODENG.2005.06.036 (2006).
Kim, S. Y., Hong, S. J., Kim, E., Lee, C. H. & Kim, G. Application of ensemble neural-network method to integrated sugar content prediction model for citrus fruit using Vis/NIR spectroscopy. J. Food Eng. 338, 111254. https://doi.org/10.1016/J.JFOODENG.2022.111254 (2023).
Luo, C., Xue, L., Liu, M., Li, J. & Wang, X. Nondestructive measurement of sugar content in navel orange based on Vis-NIR Spectroscopy. IFIP Adv. Inf. Commun. Technol. 347(4), 467–473. https://doi.org/10.1007/978-3-642-18369-0_55/COVER (2011).
Fraser, D. G., Künnemeyer, R., Jordan, R. B. & McGlone, V. A. Internal quality assessment of Mandarin fruit by vis/NIR spectroscopy. J. Near Infrared Spectrosc. 11(5), 323–332 (2003). https://opg.optica.org/abstract.cfm?uri=jnirs-11-5-323.
Cozzolino, D. et al. Effect of temperature variation on the visible and near infrared spectra of wine and the consequences on the partial least square calibrations developed to measure chemical composition. Anal. Chim. Acta. 588(2), 224–230. https://doi.org/10.1016/J.ACA.2007.01.079 (2007).
Rinnan, Å., van den Berg, F. & Engelsen, S. B. Review of the most common pre-processing techniques for near-infrared spectra. TrAC Trends Anal. Chem. 28(10), 1201–1222. https://doi.org/10.1016/J.TRAC.2009.07.007 (2009).
Anderson, N. & Walsh, K. Review: The evolution of chemometrics coupled with near infrared spectroscopy for fruit quality evaluation. J. Near Infrared Spectrosc. (2022). https://opg.optica.org/jnirs/abstract.cfm?uri=jnirs-30-1-3
Stchur, P., Cleveland, D., Zhou, J. & Michel, R. G. A review of recent applications of near infrared spectroscopy, and of the characteristics of a novel PbS CCD array-based near-infrared spectrometer. 37(4), 383–428. https://doi.org/10.1081/ASR-120016293 (2007).
Golic, M., Walsh, K. & Lawson, P. Short-wavelength near-infrared spectra of sucrose, glucose, and fructose with respect to sugar concentration and temperature. Appl. Spectrosc. 57(2), 139–145. https://doi.org/10.1366/000370203321535033 (2003).
Giovanelli, G., Sinelli, N., Beghi, R., Guidetti, R. & Casiraghi, E. NIR spectroscopy for the optimization of postharvest apple management. Postharvest. Biol. Technol. https://doi.org/10.1016/j.postharvbio.2013.07.041 (2014).
Funding
This research was supported by the Pakistan Agriculture Research Council, Agriculture Linkage Program (AE-007), Ministry of Education, Postdoctoral Initiative Program, Saudi Arabia, and Higher Education Commission of Pakistan under grants titled Establishment of National Centre of Robotic and Automation (DF-1009–31).
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The authors confirm their contribution to the paper as follows: study conception, methodology, and design: A.Z., W.S.Q., A.M.; data collection: A.Z., A.M., M.T.; analysis and interpretation of results: A.Z., A.G.A.M.; draft manuscript preparation: A.Z., E.A., M.I., W.S.Q. All authors reviewed the results and approved the final version of the manuscript.
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Zeb, A., Qureshi, W.S., Ghafoor, A. et al. Towards sweetness classification of orange cultivars using short-wave NIR spectroscopy. Sci Rep 13, 325 (2023). https://doi.org/10.1038/s41598-022-27297-2
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DOI: https://doi.org/10.1038/s41598-022-27297-2
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