Abstract
In mammals, dosage compensation of X-linked gene expression between males and females is achieved by inactivation of a single X chromosome in females, while upregulation of the single active X in males and females leads to X:autosome dosage balance. Studies in human embryos revealed that random X chromosome inactivation starts at the preimplantation stage and is not complete by day 12 of development. Alternatively, others proposed that dosage compensation in human preimplantation embryos is achieved by dampening expression from the two X chromosomes in females. Here, we characterize X-linked dosage compensation in another primate, the marmoset (Callithrix jacchus). Analyzing scRNA-seq data from preimplantation embryos, we detected upregulation of XIST at the morula stage, where female embryos presented a significantly higher expression of XIST than males. Moreover, we show an increase of X-linked monoallelically expressed genes in female embryos between the morula and late blastocyst stages, indicative of XCI. Nevertheless, dosage compensation was not achieved by the late blastocyst stage. Finally, we show that X:autosome dosage compensation is achieved at the 8-cell stage, and demonstrate that X chromosome dampening in females does not take place in the marmoset. Our work contributes to the elucidation of primate X-linked dosage compensation.
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Introduction
In mammals, dosage compensation between the single X chromosome in males and the two X in females is achieved by the process of X chromosome inactivation (XCI) which leads to the transcriptional silencing of one X in females1. In eutherian mammals the process is initiated during preimplantation development with the expression of the long non-coding RNA XIST from the future inactive X (Xi)2,3. Until recently, most studies of XCI initiation were performed in mice due to the ease in performing experiments in this animal model and the capability of mouse embryonic stem cells (mESCs) to undergo XCI when differentiated in vitro. Thus, in mice, XCI is initiated at the 4-cell stage with XIST being expressed exclusively from the paternal allele, leading to imprinted XCI (reviewed in4). At the blastocyst stage, cells from the epiblast (EPI) reactivate the inactive paternal X and, upon implantation, undergo a second round of XCI where each cell randomly inactivates either the paternal or the maternal X.
In contrast, studies of XCI initiation in humans have been hampered by difficulties in performing experiments in human embryos and lack of an adequate in vitro model system, since human embryonic stem cells (hESCs) are mostly in a post-XCI state5. Nevertheless, several differences between the process in mice and humans have been observed, including initiation of XIST expression at the 8-cell stage6, XIST expression from both X chromosomes in female or from the single X chromosome in male embryos7,8 and lack of imprinted XCI9.
Intriguingly, using single-cell RNA sequencing (scRNA-seq) data from human preimplantation embryos, Petropoulos et al.8 identified X-linked dosage compensation in female blastocysts in the absence of XCI. The authors proposed a dampening process of X-linked gene expression from both active X chromosomes as an alternative mechanism of dosage compensation in human embryos8. Using a more stringent analysis combining the aforementioned and additional scRNA-seq data, Moreira de Mello et al. excluded the hypothesis of X-chromosome dampening and showed evidence that initiation of random XCI takes place between the morula and early blastocyst stage in humans10. More recently, Zhou et al. showed that XCI initiates around the time of implantation and is not complete by day 12 of human development11. The conflicting results regarding XIST expression and timing of initiation of XCI pose a challenge in the efforts to uncover the mechanisms of dosage compensation in humans.
In this study, we aimed to investigate features of the XCI in a species that is evolutionarily closer to humans than mice. The marmoset (Callithrix jacchus) is a new world monkey widely used in biomedical research, whose evolutionary distance from humans is around 40 million years. We analyzed scRNA-seq data from 14 marmoset embryos, from zygote to the late blastocyst stage12. Using allele-specific expression analysis we show that there is no evidence of X dampening during this primate’s early development and that random XCI is also initiated at the preimplantation stage, concomitant with upregulation of the active X in male and female embryos.
Results
Embryo sexing
The dataset originally contained the transcriptome of 196 cells obtained from 14 marmoset preimplantation embryos, from the zygote to the late blastocyst stage. After filtering out cells with low-quality RNAseq data, we retained 124 cells representing all the above-mentioned stages (Table 1).
Embryos at the same stage were compared in terms of the number of Y-linked expressed genes and their respective expression levels. This strategy is able to sex embryos after complete embryonic genome activation, at the 8-cell stage12,13,14. We identified two morulas, two early- and one late-blastocyst as female; and two 8-cell embryos, one morula, one early- and one late-blastocyst as male (Supplementary Fig. S1 and Table 1).
Allele-specific expression
In order to analyze allele-specific expression, we identified heterozygous SNP positions in each embryo. For a SNP position to be considered, it had to be covered by at least 20 reads; and if a gene contained variants indicating mono and biallelic expression in the same cell, that gene was discarded from the analysis. In total, 16,311 heterozygous positions and 6985 informative genes were detected, of which 181 were located on the X chromosome.
Twenty three X-linked genes biallelically expressed were detected in male embryos (Supplementary Fig. S2). This is expected in cells from stages prior to embryonic genome activation (EGA) as maternal mRNAs are still present12,13,14. In later staged male embryos, X-linked biallelic expression may be detected due to misalignment of reads from genes located in the pseudoautosomal regions (PAR) or from genes with homologues in the Y and the X chromosomes15. In order to avoid interference from these genes, and since the marmoset PAR is not well described, we removed from the analysis all genes homologous to those in the human PAR and to those known to escape XCI (escapees), and all X-linked genes identified as biallelically expressed in at least one cell from a male embryo after the 8-cell stage (Supplementary Figs. S2 and S3). All subsequent analyses were performed using 140 informative X-linked genes resulting from this filtering step.
XIST expression
Induction of XIST expression is a hallmark of XCI initiation. In the marmoset embryos, XIST expression was first detected at the 8-cell stage in males (Fig. 1). XIST expression in female compacted morulas was significantly higher than in males at the same stage (P-value = 6.7 × 10–6). Surprisingly, at the early blastocyst stage, cells from the ICM from both male and female embryos expressed high levels of XIST. Although XIST expression persisted in the ICM of male late blastocyst, it was significantly downregulated (P-value = 4.1 × 10–5).
Allele-specific X-linked gene expression
X chromosome inactivation leads to monoallelic expression of most X-linked genes in females. Thus, in order to further investigate the process during marmoset preimplantation embryo development, we calculated the proportion of monoallelically expressed genes in each embryonic stage (Fig. 2a). As expected, male embryos showed an increase of X-linked monoallelic expression between the 8-cell and late blastocyst stages, reflecting the degradation of maternal mRNA (Fig. 2a and Supplementary Fig. S4). In female embryos, we found a significant increase of monoallelic X-linked expression between the morula and blastocyst stages (Pearson’s r = 0.56; P-value = 9.8 × 10–7, Fig. 2a; and Supplementary Fig. S4), indicative of an ongoing XCI process. The same analysis performed in chromosome 10 did not reveal any significant increase of monoallelically expressed genes in males (Pearson’s r = 0.16: P-value = 0.27) and a small correlation in females (Pearson’s r = 0.26; P-value = 0.038) (Supplementary Fig. S5).
The location of all X-linked informative SNPs and their allelic-expression patterns in female embryos are shown in Fig. 2b and Supplementary Fig. S4. It is possible to see a cluster of genes close to the XIST locus with monoallelic expression in most of the cells at the early and late blastocyst stages.
Interestingly, we detected monoallelic expression of XIST from the alternative allele in one cell of the female early blastocyst. In another cell of the same embryo, the reference allele was monoallelically expressed, although with coverage of 14 reads, below our established threshold of 20 covering reads (Supplementary Fig. S6). Nevertheless, the detection of different XIST alleles being monoallelically expressed in different cells of the same embryo indicates that random XCI is taking place. In the same early blastocyst embryo where XIST showed monoallelic expression, we also detected three other genes located in a 1 Mb window from XIST being monoallelically expressed: RNF12 and two uncharacterized loci (LOC100408393 and LOC100404148). In mice, Rnf12 is an activator of XCI, participating in the upregulation of XIST18,19,20. During the Xist accumulation process, Rnf12 is expressed exclusively from the future active X, and is downregulated very quickly after XCI is initiated21. Therefore, detection of monoallelic expression of RNF12 further suggests ongoing XCI in the marmoset early blastocyst.
X chromosome dampening
According to Petropoulos et al., dampening leads to dosage compensation in human preimplantation embryos by decreased expression of biallelically expressed X-linked genes8. In order to test the occurrence of X chromosome dampening in the marmoset, we calculated the median expression levels of biallelically expressed X-linked genes in female embryos during development (Fig. 3; Supplementary Fig. S7). If dampening of the two active X chromosomes occurred, we should detect a decrease in the median expression level of those genes during development. We found no significant differences in the expression levels of biallelically expressed X-linked genes among morula, early and late blastocyst stages (Fig. 3a), refuting the occurrence of X chromosome dampening.
Together, these data show that, while there is a growing number of monoallelically expressed genes indicative of ongoing XCI, genes that are still transcribed from both X chromosomes are not downregulated (dampened) between the morula, early and late blastocyst stages. Interestingly, we found a significant increase of the median expression levels of monoallelically expressed X-linked genes between the early and late blastocyst stages in females (Fig. 3b).
X chromosome upregulation
Upregulation of the single active X in mammalian cells was proposed as a mechanism of dosage compensation between that chromosome and the pairs of autosomes22. Indeed, X upregulation has been observed in humans, marsupials and mice10,23,24. In order to further investigate the increase in X-linked gene expression during marmoset embryonic development, we looked at the expression levels of all X-linked genes in both male and female embryos. We identified an increase in total X-linked median expression levels between morula and early blastocyst stage in both males and females consistent with upregulation of the X chromosome (Fig. 3a,b; P-value = 3.6 × 10–4 and 0.031 for females and males, respectively).
In order to test if upregulation grants the X chromosome an expression level similar to the autosomes, we calculated the X:A ratio for each cell grouped by embryonic stage and sex (Fig. 4; Supplementary Fig. S8). Our results indicate that marmoset male embryos achieve dosage compensation between X chromosome and autosomes earlier than humans: at the 8-cell stage the X:A ratio is already close to 1 (Fig. 4b), whereas in humans this is not yet achieved by the blastocyst stage10. In marmoset female morulas, early and late blastocyst, the X:A ratio was greater than 1 but less than 2 (Fig. 4b), consistent with the occurrence of both X upregulation and incomplete XCI as observed in human embryos10. Moreover, X:A ratios were significantly different between male and female in all analyzed stages (Fig. 4a; morula, P-value = 1.9 × 10–5; early blastocyst, P-value = 5.4 × 10–5; late blastocyst P-value = 4.8 × 10–7), further indicating incomplete XCI. Together with the analysis of allele-specific expression level (Fig. 3), these results suggest that X upregulation takes place and is complete earlier than XCI during marmoset preimplantation embryonic development.
Discussion
Despite remarkable advances in dissecting the mechanisms of XCI in mice, much less is known about the process during human embryonic development. Still, important differences between the process in the two species have been identified, including lack of imprinted XCI in humans9 and different timing of random XCI initiation6,7,8,10, although there is still disagreement regarding the latter. While some studies have identified initiation of random XCI in pre and perimplantation embryo stages5,10,11, others argue that dosage compensation in human blastocysts is achieved by X chromosome dampening8,25. Although the hypothesis of dampening has been also refuted in pigs26, the study of X-linked dosage compensation in an animal model evolutionarily closer to humans may contribute in elucidating these issues. Therefore, here we sought to characterize the dynamics of X chromosome regulation during preimplantation development in another primate, the marmoset (Callithrix jacchus).
By analysing scRNA-seq data, we were able to perform a chromosome-wide analysis of X-linked genes in marmoset preimplantation embryos. Our results are consistent with an ongoing process of XCI in the early blastocyst of the female marmoset, characterized by an increase in XIST expression and in the percentage of monoallelically expressed X-linked genes, as observed in human preimplantation embryos10,11.
Monoallelic expression of alternative XIST alleles in different cells of the same early blastocyst embryo suggests that XCI takes place in a random pattern. This is expected since the expression data were of cells from the ICM and adult marmoset females present random XCI27. In mice, extraembryonic tissues present imprinted inactivation of the paternal X28, whereas random XCI is present in human placenta9. Due to lack of scRNA-seq data from trophectoderm cells, we were not able to exclude the existence of imprinted XCI in the marmoset.
Male marmoset early blastocysts showed upregulation of XIST comparable to that in female cells at the same stage, while in male late blastocysts XIST expression was significantly decreased. Interestingly, XIST expression has also been detected in rhesus macaques (Macaca mulatta) and human male blastocysts6,7,29, suggesting that XIST expression in preimplantation male embryos is a feature of primates.
We further investigated X upregulation by analysis of the X:A ratio. We showed that male marmoset embryos achieve X:A dosage compensation during the preimplantation development. Accordingly, female marmoset embryos presented an X:A ratio between 1 and 2, suggestive of ongoing XCI in upregulated X chromosomes. In contrast, human embryos achieve X:A dosage compensation only after implantation11.
Finally, we investigated the existence of X dampening in the marmoset embryos which should lead to decreased expression levels of biallelically expressed genes during development. Instead, we found no significant differences in expression levels of those genes between the morula and early/late blastocyst stages, refuting the occurrence of dampening.
In conclusion, despite the limited number of female embryos, our work adds to the panorama of XCI in primates, and establishes the marmoset as a model system where, as in humans, X-linked dosage compensation between males and females is achieved by random XCI which starts during preimplantation embryo development.
Methods
Transcriptome data
The marmoset (Callithrix jacchus) scRNA-seq raw data is available on the ArrayExpress platform (https://www.ebi.ac.uk/arrayexpress/) under accession number E-MTAB-7078. This dataset comprises the transcriptome of 196 cells obtained from 14 preimplantation embryos (from zygote to late blastocyst stage, Table 1).
Quality filter and mapping
Adapters and low-quality reads were removed using cutadapt30 v1.18. In addition to removing bases with a phred score lower than 20, sequences with poor GC content were also trimmed out. Finally, reads shorter than 30 bp were discarded.
After trimming, TopHat231 v2.1.1 was used to map reads to the marmoset assembly Callithrix jacchus-3.2 and its respective annotation (obtained from NCBI Assembly https://www.ncbi.nlm.nih.gov/assembly/, NCBI Callithrix jacchus Annotation Release 102, GCA_000004665.1). SAMTools32 v1.9 was used to remove unmapped reads and secondary alignments as well as for sorting. The numbers of reads assigned to each gene were counted with HTSeq using mode intersection-nonempty33. We used the latest marmoset gene annotation from May 2020 (Callithrix_jacchus_cj1700_1.1).
Data pre-processing
The marmoset preimplantation embryo dataset contained originally the transcriptome of 196 isolated cells obtained from 14 marmoset preimplantation embryos, from the zygote to the late blastocyst stage. In order to identify cells with low-quality data, a Seurat object was created considering cells with at least 200 detected genes where those genes should be expressed in at least three cells. Through visual analysis we decided to retain only cells with more than 2 × 106 and less than 5.1 × 106 reads mapped to genes (Seurat package version 3.2.2)34. After filtering out cells with low-quality data, we retained 124 good quality cells still representing all of the above-mentioned stages.
Sexing
In order to determine the sex of each embryo, we combined two strategies: (1) counting the number of Y-linked expressed genes, and (2) measuring the expression levels of those genes for each individual embryo. To be considered as an expressed gene, the normalized TPM level should be greater than or equal to one. All Y-linked genes detected in embryos at the zygote and 4-cell stages were removed from the analysis, as the transcriptome at these stages is mainly composed of maternal mRNA deposited during the oogenesis. It is not expected but also not unusual to detect expression of Y-linked genes from female transcriptomes due to the similar regions between the X and Y chromosomes in mammals (mainly the PAR regions). After the filtering steps we retained five protein coding genes located on the non-recombining region of the Y chromosome. Finally, we looked for statistical differences by comparing all embryos at the same stage for both the number of Y-linked genes and their expression level with the unpaired Wilcoxon test.
Genotyping and variant calling
Genotypes for each embryo were constructed by merging the transcriptome data of all cells from the same embryo and identifying heterozygous positions with the use of VarScan35 v2.3.9. Variants were also identified in individual cells with VarScan v2.3.9 and filtered with bcftools36 v1.9 in order to keep only biallelic single nucleotide polymorphisms in subsequent analyses. Each cell’s variants were then compared to the respective embryo genotype, a strategy that allows for the identification of differential monoallelic expression in individual cells from the same embryo. Only variants covered by 20 or more reads were considered. GATK37 v4.1.0.0 VariantsToTable was used to convert the VCF output from VarScan into a readable format for data analysis.
Gene calling and allelic expression
Allelic expression was inferred from the allelic frequency of each SNP. According to Borel et al., an allelic frequency of 0–20% or 80–100% of either the reference or alternative allele corresponds to monoallelic expression, while an allelic frequency of 20–80% indicates biallelic expression38.
According to the position of each variant, the corresponding gene was identified. Each gene’s allele-specific expression was inferred based on the detected SNPs. When all of the gene’s variants presented monoallelic expression, the gene’s expression was assumed to be monoallelic. The same applies to biallelic expression from the SNPs. If variants from the same gene were not in agreement regarding the allelic expression (mono or biallelic), that gene was discarded from the analysis.
Allelic expression pattern
The proportion of mono and biallelically expressed genes was calculated as a percentage of the total informative genes in each cell. Informative genes correspond to those genes whose expression can be inferred from their SNPs allele frequency. Total informative genes represent the sum of mono and biallelically expressed genes.
Gene expression levels
To test the existence of dampening, we extracted the expression levels only for those genes whose expression was found to be biallelic. We then calculated the median expression levels in each cell, taking into consideration the biallelic genes with expression levels greater than 0.1 FPKM (fragments per kilobase of transcript, per million). We employed the same strategy to analyze the expression levels of genes that were found to be expressed monoallelically.
For the evaluation of global expression levels from the X chromosome, we considered all genes with expression levels greater than 0.1 FPKM regardless of being informative (excluding the ones removed from the allele-specific analyses). We then found the median expression levels for each cell and grouped the results according to each embryonic stage.
X:A ratio
We calculated the median X:A ratio by using all expressed X-linked and autosomal genes (expression level greater than 0.1 FPKM). We also calculated the median X:A ratios according to the method described by Fukuda et al., and only genes with expression levels greater than 0.1 FPKM were considered (excluding the ones removed from the allele-specific analyses)39. Using the ‘sample’ function in R, we randomly selected 100 X-linked genes and 100 autosomal genes, for which an X:A ratio was calculated for each cell. This analysis was repeated by bootstrapping with 2000 replications, allowing for the calculation of median X:A ratios (Supplementary Fig. S8).
Statistics and visualization
Data visualization analyses and statistical tests were performed using R40 v3.6.3 and ggplot2 package41 v3.3.2.
Code availability
Scripts used in this work can be provided on reasonable request.
References
Lyon, M. F. Sex chromatin and gene action in the mammalian X-chromosome. Am. J. Hum. Genet. 14, 135–148 (1962).
Brown, C. J. et al. A gene from the region of the human X inactivation centre is expressed exclusively from the inactive X chromosome. Nature 349, 38–44 (1991).
Panning, B., Dausman, J. & Jaenisch, R. X chromosome inactivation is mediated by Xist RNA stabilization. Cell 90, 907–916 (1997).
Rebuzzini, P., Zuccotti, M. & Garagna, S. X-Chromosome inactivation during preimplantation development and in pluripotent stem cells. Cytogenet. Genome Res. 160, 283–294 (2020).
Vallot, C. et al. XACT noncoding RNA competes with XIST in the control of X chromosome activity during human early development. Cell Stem Cell 20, 102–111 (2017).
van den Berg, I. M. et al. X Chromosome inactivation is initiated in human preimplantation embryos. Am. J. Hum. Genet. 84, 771–779 (2009).
Okamoto, I. et al. Eutherian mammals use diverse strategies to initiate X-chromosome inactivation during development. Nature 472, 370–374 (2011).
Petropoulos, S. et al. Single-Cell RNA-Seq reveals lineage and X chromosome dynamics in human preimplantation embryos. Cell 165, 1012–1026 (2016).
de Mello, J. C. M. et al. Random X inactivation and extensive mosaicism in human placenta revealed by analysis of allele-specific gene expression along the X chromosome. PLoS ONE 5, 1–8 (2010).
de Mello, J. C. M., Fernandes, G. R., Vibranovski, M. D. & Pereira, L. V. Early X chromosome inactivation during human preimplantation development revealed by single-cell RNA-sequencing. Sci. Rep. 7, 10794 (2017).
Zhou, F. et al. Reconstituting the transcriptome and DNA methylome landscapes of human implantation. Nature 572, 660–664 (2019).
Boroviak, T. et al. Single cell transcriptome analysis of human, marmoset and mouse embryos reveals common and divergent features of preimplantation development. Development (Cambridge) 145(21), dev167833. https://doi.org/10.1242/dev.167833 (2018).
Chitwood, J. L., Burruel, V. R., Halstead, M. M., Meyers, S. A. & Ross, P. J. Transcriptome profiling of individual rhesus macaque oocytes and preimplantation embryos. Biol. Reprod. 97, 353–364 (2017).
Guo, F. et al. The transcriptome and DNA methylome landscapes of human primordial germ cells. Cell 161, 1437–1452 (2015).
Raudsepp, T. & Chowdhary, B. P. The eutherian pseudoautosomal region. Cytogenet. Genome Res. 147, 81–94 (2016).
Lahn, B. T. & Page, D. C. Four evolutionary strata on the human X chromosome. Science 286(5441), 964–967. https://doi.org/10.1126/science.286.5441.964 (1999) (Erratum in: Science 1999 Dec 17;286(5448):2273. PMID: 10542153).
Kelkar, A., Thakur, V., Ramaswamy, R. & Deobagkar, D. Characterisation of inactivation domains and evolutionary strata in human X chromosome through Markov segmentation. PLoS ONE 4(11), e7885. https://doi.org/10.1371/journal.pone.0007885 (2009).
Jonkers, I. et al. RNF12 is an X-encoded dose-dependent activator of X chromosome inactivation. Cell 139, 999–1011 (2009).
Barakat, T. S. et al. RNF12 activates Xist and is essential for X chromosome inactivation. PLoS Genet. 7, 1–12 (2011).
Gontan, C. et al. RNF12 initiates X-chromosome inactivation by targeting REX1 for degradation. Nature 485, 386–390 (2012).
Barakat, T. S. et al. The trans-activator RNF12 and cis-acting elements effectuate X chromosome inactivation independent of X-pairing. Mol. Cell 53, 965–978 (2014).
Ohno, S. Sex Chromosomes and Sex-linked Genes (Springer, 1967). https://doi.org/10.1111/j.1439-0272.1973.tb00879.x.
Wang, F. et al. Regulation of X-linked gene expression during early mouse development by Rlim. Elife 5, 1–19 (2016).
Mahadevaiah, S. K., Sangrithi, M. N., Hirota, T. & Turner, J. M. A. A single-cell transcriptome atlas of marsupial embryogenesis and X inactivation. Nature https://doi.org/10.1038/s41586-020-2629-6 (2020).
Sahakyan, A. et al. Human naive pluripotent stem cells model X chromosome dampening and X inactivation. Cell Stem Cell 20, 87–101 (2017).
Ramos-Ibeas, P. et al. Pluripotency and X chromosome dynamics revealed in pig pre-gastrulating embryos by single cell analysis. Nat. Commun. 10, 1–17 (2019).
Machado, F. B. et al. 5meCpG epigenetic marks neighboring a primate-conserved core promoter short tandem repeat indicate X-chromosome inactivation. PLoS ONE 9, 1–13 (2014).
Borensztein, M. et al. Xist-dependent imprinted X inactivation and the early developmental consequences of its failure. Nat. Struct. Mol. Biol. 24, 226–233 (2017).
Tachibana, M. et al. X-chromosome inactivation in monkey embryos and pluripotent stem cells. Dev. Biol. 40, 1301–1315 (2012).
Martin, M. Cutadapt removes adapter sequences from high-throughput sequencing reads. EMBnet.journal 17, 10–12 (2013).
Kim, D. et al. TopHat2: accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions. Genome Biol. 14, 957–961 (2013).
Li, H. et al. The sequence alignment/map format and SAMtools. Bioinformatics 25, 2078–2079 (2009).
Anders, S., Pyl, P. T. & Huber, W. HTSeq-A python framework to work with high-throughput sequencing data. Bioinformatics 31, 166–169 (2015).
Stuart, T. et al. Comprehensive integration of single-cell data. Cell 177(7), 1888–1902.e21. https://doi.org/10.1016/j.cell.2019.05.031 (2019).
Koboldt, D. C. et al. VarScan: Variant detection in massively parallel sequencing of individual and pooled samples. Bioinformatics 25, 2283–2285 (2009).
Li, H. A statistical framework for SNP calling, mutation discovery, association mapping and population genetical parameter estimation from sequencing data. Bioinformatics 27, 2987–2993 (2011).
McKenna, A. et al. The genome analysis toolkit: A MapReduce framework for analyzing next-generation DNA sequencing data. Genome Res. 20, 1297–1303 (2010).
Borel, C. et al. Biased allelic expression in human primary fibroblast single cells. Am. J. Hum. Genet. 96, 70–80 (2015).
Fukuda, A., Tanino, M., Matoba, R., Umezawa, A. & Akutsu, H. Imbalance between the expression dosages of X-chromosome and autosomal genes in mammalian oocytes. Sci. Rep. 5, 2–10 (2015).
R Core Team. R: A Language and Environment for Statistical Computing (R Foundation for Statistical Computing, 2017).
Wickham, H. ggplot2: Elegant Graphics for Data Analysis (Springer-Verlag, 2009).
Acknowledgements
This work was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, fellowship 2018/14.856-8, ALC). J.C.M.M is supported by the Oncode Institute.
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A.L.C. design, collection and assembly of data, data analysis and figures preparation; J.C.M.M. data analysis and figure preparation; J.G. data analysis; L.V.P. conception, design and supervision of the work. All authors wrote the manuscript.
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Cidral, A.L., de Mello, J.C.M., Gribnau, J. et al. Concurrent X chromosome inactivation and upregulation during non-human primate preimplantation development revealed by single-cell RNA-sequencing. Sci Rep 11, 9624 (2021). https://doi.org/10.1038/s41598-021-89175-7
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DOI: https://doi.org/10.1038/s41598-021-89175-7
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