Abstract
Bacterial cells have characteristic spatial and temporal scales. For instance, Escherichia coli, the typical rod-shaped bacteria, always maintains a relatively constant cell width and cell division time. However, whether the external physical perturbation of cell width has an impact on cell division time remains largely unexplored. In this work, we developed two microchannel chips, namely straight channels and ‘necked’ channels, to precisely regulate the width of E. coli cells and to investigate the correlation between cell width and division time of the cells. Our results show that, in the straight channels, the wide cells divide much slower than narrow cells. In the ‘necked’ channels, the cell division is remarkably promoted compared to that in straight channels with the same width. Besides, fluorescence time-lapse microscopy imaging of FtsZ dynamics shows that the cell pre-constriction time is more sensitive to cell width perturbation than cell constriction time. Finally, we revealed a significant anticorrelation between the death rate and the division rate of cell populations with different widths. Our work provides new insights into the correlation between the geometrical property and division time of E. coli cells and sheds new light on the future study of spatial–temporal correlation in cell physiology.
Similar content being viewed by others
Introduction
Bacterial cells usually have conservative shapes, sizes1, and division time2. The combination of these spatial and temporal properties has evolved in coordination to maximize the survivability and reproduction efficiency of the species3,4. Three kinds of models have been proposed to explain how bacterial cells control their size homeostasis throughout the cell cycle5. The first one is the ‘timer’ model, in which bacterial cells would grow for a specific amount of time between two successive divisions6. The second one is the ‘sizer’ model, in which cells commit to division on reaching a size threshold7. The third is the well-known ‘adder’ model, in which bacterial cells achieve their size homeostasis by adding a constant length8, volume9, surface area10, or ‘adder-per-origin’11 before division, irrespective of cell size at birth. However, these pioneering works postulate that cells grow and divide under the natural condition without any size perturbation. For instance, Escherichia coli, the rod-shaped bacteria always grow along the long axis of the cell and maintain its cell width constant during the cell cycle. However, whether those models still work out when their cell width is disturbed, remains to be explored.
In recent years, much progress has been made in understanding the E. coli cellular physiology when its cell geometry is artificially disturbed. These perturbations can be achieved through various biological, chemical, and physical methods, such as cytoskeletal mutation12,13, chemical treatment with A22 (S-3,4-dichlorobenzyl-isothiourea)14,15, or external physical constraints16,17. Männik et al. squeezed E. coli cells into irregular shapes by narrow silicon channels and showed that cells manage to divide into two equal-sized daughter cells regardless of their abnormal shapes18. Wu et al. studied the Min oscillation pattern of E. coli with large size and diverse geometric shapes using A22 and cephalexin combined with agarose microchambers19. These experiments have revealed crucial roles that E. coli cell shape and size play in cellular physiology; however, it is still largely unknown whether the perturbation of cell width will significantly affect their division time.
Notably, bacterial cell division is a complex process that contains numerous molecular events, including chromosome replication and segregation20, division site positioning21, septum assembly22, cell constriction coupled with cell wall synthesis23, some of which might be sensitive to cell width. For instance, the septum assembly and cell constriction of E. coli are facilitated by cell divisome, a dynamic multiprotein assembly localizing at mid-cell to synthesize new peptidoglycan and to constrict cell envelope24. In E. coli, the cell divisome consists of at least 34 different proteins25, where FtsZ is required for cytokinesis, forming a ring-shaped structure that treadmills along the cellular circumference, mediated by membrane-attached proteins FtsA and ZipA26,27. This ‘Z-ring’ recruits other division proteins and generates contractile force for cell envelope constriction28. Previous researches show that Z-ring positioning is robust against the fluctuation of cell width29. However, other cell division related processes, such as Min oscillation19,30, chromosome replication31,32 and FtsZ turnover and polymerization dynamics33 are also subjected to the modulation of cell width. Despite these previous works, it remains largely unclear whether perturbation of cell width will affect cell division time of E. coli, due to the lack of proper experimental methods to investigate the division time of cells with different width systematically.
To address this issue, we developed two easy-to-use microchannel chips that enable us to monitor the correlation between cell width and cell division time of E. coli at the single-cell level. Using the chip of straight channels with various widths (0.8–2.8 μm), we found that there is a significant positive correlation between individual cell division time and its width. We then asked whether local constrains on cell width can lead to a significant effect on cell division time as well. To obtain local constraints on cell width, we developed microchannels with fixed width and local constriction regions along the channels. We discovered that, compared to the straight channels, the channels with the same width and local constriction lead the E. coli cells to much shorter division time. We then used fluorescence time-lapse microscopy to track the FtsZ dynamics and found that the cell width perturbation has a major impact on the time duration of both pre-constriction and constriction phases of the cell cycle, and the impact is more significant on the former one than the second. Finally, we discovered a remarkable anticorrelation between the death rate and the division rate of the cell population with various cell widths. Our work, for the first time, revealed how physical modulation of cell width leads to the significant change of cell division time and survivability of E. coli, which will shed new light on further exploration of the interplay between cell morphology and physiology.
Results
Sculpting living cells with defined widths
Previous studies have introduced various microfluidic and micropattern techniques to explore cell physiology with arbitrary morphology18,29. For instance, the microfluidic ‘mother machine’ system has been developed to enable long-term observation of cell growth in a chemostat environment with single-cell resolution34. However, if cells with large size and deformability enter the channel of mother machine, the diffusion of nutrition will be largely blocked by the cells closed to the entry, which will lead the other cells in the channel to a severe lack of nutrition (Supplementary Fig. S1a). The agarose microchambers, on the other side, can provide homogenous nutrition supply despite the aberrant cell morphology19,35. However, after a period of growth and division, the cells grow, divide, squeeze the microchambers, and eventually cause the microstructure to deform or collapse, which makes it difficult to obtain high-quality imaging of the growth and division of cells in long term (Supplementary Fig. S1b).
In this study, we developed an easy-to-use ‘sandwich’ microchannel chip, to precisely control the widths of E. coli cells and obtain high-quality and long-term cell division imaging. This microchannel chip consists of an agarose pad layer, a thin PDMS layer with microchannels, and two coverslips (Fig. 1a). The agarose pad is used to supply nutrients containing the Luria–Bertani medium with A22, an antibiotic that antagonizes the dynamics of bacterial cytoskeleton protein MreB, which facilitates the deformation of cells. The microchannels in the PDMS layer are 1 μm deep, 60 μm long, and with various widths ranging from 0.8 to 2.8 μm, applied to sculpture the morphology of cells with determined width. The coverslips on the top and bottom prevent the drying of the agarose layer and offer the support of the sandwich structure. Due to the function of A22, E. coli cells seeded in the microchannels gradually grow into a round shape and eventually adapt to the border of the channels. With their widths limited by the channels, cells grow and divide along the long axis of the channels. We then take time-lapse images of cells living in the channels every two minutes for two hours (Fig. 1b). Although the deformed E. coli cells in our experiment are one to ten times larger in volume than wild type cells, most of them manage to divide around the volumetric center, which indicates the remarkable robustness of cell division site determination even with such significant perturbation of cell size in our work.
Wide cells divide much slower than narrow cells
We then investigated whether and how cell width perturbation influences cell growth and division time of E. coli. Since the depth of the channel is fixed, the measurement of cell biomass growth rate is equivalent to measuring the growth rate of its cell area by a series of time-lapse images. Our data shows that, despite their widths, all the cells in microchannels follow exponential growth law: ST = S0 eαT, where S0 and ST indicate the cell area at the initial time T0 and the time T, respectively, and α indicates the biomass growth rate for an individual cell. The statistical result shows that cells exhibit a relatively uniform growth rate in biomass despite the variation of initial cell area of cells with different widths (α = 0.019 ± 0.004 min−1, Mean ± SD, P > 0.05, Fig. 2a and Supplementary Fig. S2). Previous research shows that severe mechanical deformation could lead to slower growth18. However, our results indicate that the biomass growth rate of E. coli is robust to the change of cell width by this gentle physical constrains.
Given the growth rate of biomass maintains undisturbed when cell width changes, we then explored whether cell division time t is also robust to the change of cell width w. To this end, we applied a threshold method to determine the initiation and accomplishment of the cell division process and calculate the cell division time as the time duration between two successive cell division events (Supplementary Fig. S3). The results of the division time of cells growing in channels with different widths show that there is a significant positive correlation between cell division time t and cell width w (Pearson’s r = 0.98; Fig. 2b). Generally, wide cells divide much slower than narrow cells. For instance, those cells at 0.8 μm spend less than 20 min for one division, while those at 2.8 μm have an average division time of 93 min, which is about 4.7 times of the former one. It is also worth noting that wide cells have a larger variation of division time than that of narrow cells (CV0.8 μm = 7.8%; CV2.8 μm = 14%), see in Supplementary Table S3. The discovery of the constant biomass growth rate and positively correlated cell division time with the width micro-channels offers a straightforward explanation to the observation that E. coli cell size at the end of cell division (SE) increases with the width of channels (see “Methods” and Supplementary Fig. S4a).
Local constriction of the cell width helps accelerate the process of cell division
As we have shown that changing the width of entire cells of E. coli will result in a significant change of cell division time, it is of great interest to ask whether this effect could be local, that is, whether local modification of cell width has an impact on cell division time? To answer this question, we developed a dedicated sandwich chip with ‘necked’ channels: all ‘necked’ channels with fixed width of 3.4 μm and periodic local constrictions (referred as ‘necks’). We define \(\varphi\) = 2∆/w as the constriction ratio, where \(\Delta\) and w represent the half-constriction distance and the width of the channel, respectively (Fig. 3a). We applied fluorescence time-lapse microscopy to follow and compare the growth and division of cells in the straight and ‘necked’ channels (Fig. 3b). We firstly investigated whether local regulation of cell width affects the biomass growth rate of E. coli. Our results show that cells in ‘necked’ channels also have a uniform growth rate (α = 0.018 ± 0.003 min−1, Mean ± SD, P > 0.05, Supplementary Fig. S2b), which is in line with the result of cells in straight channels (P > 0.05). This indicates that the local constriction of cell width has no obvious effect on the biomass growth of cells.
We then ask whether these necks influence on cell division time. Thus, we compared the division time t of those cells in the ‘necked’ channels that were locally restricted by the necks throughout their cell division process with the cells in neck-free straight channels of the same width. In this work, only those cells who managed to accomplish at least one full cell division in our experimental time are considered. The result shows that the ‘necked’ cells divide significantly faster than those in straight channels (****P < 0.0001; Fig. 3c). Quantitatively speaking, cell division time in straight channels (116.3 ± 25.8 min, Mean ± SD, \(\varphi\) = 0) is nearly twice larger than the ‘necked’ ones (55.7 ± 12.7 min, Mean ± SD, \(\varphi\) = 0.66). This result indicates that local physical constriction indeed has a significant impact on the cell division time of E. coli.
The pre-constriction phase of cell division process is more sensitive to cell width perturbation
Our work has shown that cell division time can be largely affected by the width regulation of the whole cell or just on the local regions of the cells. Then, given the complicated processes related to the cell division, we try to determine which stages of the cell division process are sensitive to the perturbation of cell width. Previous research shows that the time spent on the ‘D’ period of the cell cycle increases with the increasing cell width11. A straightforward explanation is that, given that the Z-ring constriction is rather constant under the same growth condition33, the increase in cell width leads to larger circumference and an increase in cell constriction time. However, whether this is the only or major route for cell width to influence division time is to be explored. To precisely quantify the impact of cell width on cell division machinery, we constructed a fusion fluorescent protein FtsZ-gfpmut2 as a marker molecule to track the dynamics of FtsZ molecules and Z-ring during the whole cell cycle. During cell division process, FtsZ molecules undergo a multi-step process, including dispersion in cytoplasm, aggregation on cell membrane, formation of Z-ring, constriction of Z-ring and eventually dissociation (Supplementary Fig. S5a). We divide the whole cell division process accordingly into two major phases, namely the pre-constriction phase (phase I) and the constriction phase (phase II). Here, phase I begins with the end of the last division and ends up forming a matured divisome (Z-ring or Z-arc), and phase II begins with the inward constriction of the divisome and ends with the dissociation of the FtsZ clusters eventually36. The criterion to determine the two phases for both straight and ‘necked’ channels is illustrated in Fig. 4a and Supplementary Fig. S5c. τ1 and τ2 denote the time duration of phase I and phase II, respectively.
We then apply fluorescence time-lapse microscopy to investigate the strength of the impact of cell width on τ1 and τ2. In straight channels, we find that although both τ1 and τ2 are increasing with cell width. τ1 increases much faster than τ2 (**P < 0.01, Fig. 4b). The ratio of time spent on both phases ε, i.e., ε = τ1/τ2, ranging from 1.1 to 2.0 as the cell width increases (Supplementary Fig. S6). On the other hand, in the ‘necked’ channels, τ1 is reduced significantly compared to cells in straight channels (****P < 0.0001). In the meanwhile, τ2 remains relatively constant (P > 0.05, 25.6 ± 7.9 min, Mean ± SD, Fig. 4c). When constriction ratio \(\varphi\) increases to 0.66, ε decreases significantly from 2.8 to 1.7 (Supplementary Fig. S7).
Cell width perturbation has a significant impact on the population death rate
In our experiment, we observed that some cells die, whose nucleoid fluorescence dissipate during the experimental period (Supplementary Fig. S8). It is of great interest to ask whether the perturbation of cell width affects not only division time but also survivability of cells. And if so, is there a correlation between population death rate and division rate when cell width is variable? To answer these questions, we measure the death rate θ of cell population and their according division rate δ with various cell width w. The death rate θ is defined as θ = m/NT, where N is the total cell number and m is the number of cells that die during a period of T. The division rate δ is defined as δ = 1/t, where t is the division time of individual cell. In straight channels, we find that when cell width changes from 0.8 μm to 2.8 μm, the division rate δ decreases from 3 to 0.6 h−1, the death rate θ increases from 0 to 5.3 \(\times\) 10–2 h−1 (Fig. 5a). In ‘necked’ channels, when \(\varphi\) increases to 0.84, the death rate θ decreases from 5 \(\times\) 10–2 to 0.6 \(\times\) 10–2 h−1, while the division rate δ increases from 0.55 to 1.14 h−1 (Fig. 5b). To further explore the correlation between θ and δ, we plot lnθ versus lnδ for both straight and ‘necked’ channels. Result shows that there is a significant anticorrelation between the death rate and the division rate of the cell population both for straight channels (Person’s r = − 0.96, Fig. 5a inset) and ‘necked’ channels (Person’s r = − 0.89, Fig. 5b inset).
Discussion
To summarize, in this work we investigate the correlation between cell width and division time of E. coli as a model system to reveal the crucial impact of geometrical properties on essential physiology of bacterial cells. By developing easy-to-use sandwich microchannel chips, we achieved both precise regulation of the width of the whole cell and local regions of the cell. Using the microchannel chips and fluorescence microscopy we found that the division time of E. coli is significantly correlated with cell width. The discovery that local necks can also modulate cell division rate indicates that local constriction of cell width is enough to influence cell division time. Besides, by tracking the dynamics of fluorescent FtsZ proteins, we found that the perturbation on cell width has a greater effect on pre-constriction phase. Finally, we revealed a significant anticorrelation between the death rate and the division rate of the cells with different widths. To the best of our knowledge, our work is the first systematic study on the correlation between cell width and cell division time, which will help future exploration of spatial–temporal interplay in cell physiology.
Proper spatial localization of cell divisome is of great importance to cell physiology and survivability. In our work, we discovered most cell deaths correlated with failure in forming a matured divisome. Geometrical constrictions may lead to non-negligible impact on the positioning of divisome (supplementary Fig. S10) and essential dynamic processes related to cell division, such as nucleoid occlusion37,38 and MreB localization39,40. The FtsZ-ZapA-ZapB-MatP linkage may play a role in coordinating division with nucleoid segregation41. Whether the change of cell width could influence the distance of successive replisomes and the number of replisomes42? The Min oscillation system is also reported with a timing effect on cell division43. How do the interactions between those concurrent processes affect the temporal scale of cell division when cell width is variable44? Those questions are yet to be explored.
Methods
Strains and growth medium
All the strains used in this study were E. coli K-12 MC4100, constructed, sequenced, and extensively tested by Ariel B. Lindner’s laboratory. Detailed information on strain genotype is included in Table S1. The FtsZ-gfpmut2 expression strain used in the steady-state inhibition experiment is based on a strain with a plasmid pJC85::pBAD42 carrying an extra copy of FtsZ under Plac promoter. E. coli growth experiments were performed in the LB medium. 25 mg/ml Kanamycin was used to grow the strains with respective resistance markers. The expression of FtsZ-gfpmut2 protein was induced by 50 ug/ml IPTG. Images were shot when cells were grown in LB solid medium (5% agarose) supplemented with 12 μM A22. Detailed information about the medium component is included in Supplementary Table S2.
Micro-channel PDMS chip preparation
We developed a micro-channel chip that consisted of a thin polydimethylsiloxane (PDMS) layer, an agarose gel layer and two glass coverslips (CITPGLAS. 24 \(\times\) 50 mm, thickness 0.13–0.16 mm). PDMS microchannel was prepared by replicating structures from a silicon mold, which contained silicon relief structures with a micron-feature size fabricated using electron beam lithography and dry etching techniques with 100 nm spatial resolution. The silicon mold was treated with the vapor of trimethylchlorosilane for over 15 min before use to prevent the adhesion of PDMS and silicon mold. PDMS oligomers (184 silicone elastomer bases, Sylgard, USA) and cross-linking agent (184 silicone elastomer curing agent, Sylgard, USA) were mixed at a 10:1 mass ratio to prepare PDMS pre-polymer solution. The solution was first pumped to a rough vacuum in a vacuum bell jar to remove air bubbles. Then a droplet of mixed PDMS was poured onto the silicon mold. Thereafter we used a spin-coater to make a PDMS thin film with a spin program: in the first pulse at 500 rpm for 30 s and in the second pulse at 1400 rpm for 10 s Then the PDMS film was cured for about 2 h at 75 °C in an oven (Thermo Scientific, Hera Thermo), which resulted in a thin layer of PDMS film. Then the PDMS film was carefully peeled away from the mold, transferred to a glass coverslip (see Supplementary Fig. S10 for the SEM image of the micro-channels with “necks”). The coverslip was ultrasonically cleaned in ethanol for 10 min and dried by N2 gas. To clean the surface of the PDMS film and enhance the surface hydrophilicity, we applied an oxygen plasma treatment for one minute. A droplet of E. coli cell suspension was applied onto the PDMS film. To confine the cells and to provide the nutrition to the bacterial cells, a prepared agarose pad was covered on it containing 50 ug/ml IPTG, 12 μM A22. Then we put another glass coverslip onto the top of the agarose pad and used solid paraffin wax to seal the gap between the coverslips. Cells were confined to the PDMS micro-chamber and grown at 30 °C on a temperature-controlled microscope stage for 3 h.
Cell preparation
Before time-lapse imaging, cells were picked from a single colony on an agar plate which was streaked no more than 7 days before use. The cells were inoculated into 4 mL LB liquid medium with carefully selected antibiotics. After shaking for 12 h at 37 °C in a thermo-state shaker, cells were diluted 100 folds into 2 mL of fresh liquid medium. Incubate again in a shaker at 37 °C till OD600 = 0.1–0.4. 1 µl of the bacterial culture liquid was then pipetted onto the PDMS chips on a coverslip. The droplet was then immediately covered with a 5% agarose pad containing LB, 12 µM A22 and 50 ug/ml IPTG.
Microscopy and image acquisition
We performed simultaneous phase-contrast imaging and epifluorescence imaging on an inverted fluorescence microscope (Nikon Ti-E) with Perfect Focus (TI-PFS-CON2), 100 × oil immersion objective (Nikon plan Apo VC, numerical aperture = 1.4). The microscope was enclosed by a custom-made chamber that was pre-heated and kept at 30 °C. Fluorescence was excited by a lamp (Nikon C-HGFIE) through a neutral density filter. For excitation of GFP signal, cells were illuminated through a GFP filter cube (λex/λbs/λem = 450–490/495/500–550 nm). The fluorescence signal was recorded by a Cool SNAP HQ2 camera. The FtsZ dynamics was captured with a time-interval of 2 min. Exposure time for GFP and TXRED florescence both was 3 s, and the bright field was 0.5 s. ND was set as 1/8. Images were recorded by software named ‘MetaMorph’. In our experiment, limited by the size of micro-channels and the scope of microscopic imaging, we can only follow the growth and division of cells for about 3 h, after which over 80% of the cells grow out of the channel or the scope of imaging. Therefore, the results we discussed are based on the experimental data within the first two hours after cell loading and 1 h adaptation.Given we perform all the experiments strictly with same conditions, such as temperature, A22 concentration, cell pre-culture and adaptation time, etc., and the only difference between the cells is the geometric constraints such as the channel width and neck width, it is reasonable to attribute the variation of cell size and their divergence to the difference of geometric constraints but not to the other experimental conditions.
Image processing
Image processing was performed using FIJI software. Toolboxes were used for image analysis, such as contrast and brightness adjustments. Phase-contrast images and fluorescent images of FtsZ dynamics were adjusted for contrast. When necessary, images were background-corrected using a rolling ball with radius 50 pixels, and median-filtered for 2 pixels with merged fluorescent images. Images were background-subtracted for viewing purposes. Unaltered images were used for quantitative processing in all cases.
Statistical analysis
The quantitative data are expressed as Mean ± SD. Comparisons between two or more specific sets of data are analyzed by using One-way analysis of variance (ANOVA) test. For all data analyses, the P value of < 0.05 are considered statistically significant, and P value of > 0.05 are considered no statistically significant.
Data availability
The data generated during the current study is included in this article and its supplementary files.
References
Young, K. D. The selective value of bacterial shape. Microbiol. Mol. Biol. Rev. 70, 660–703. https://doi.org/10.1128/MMBR.00001-06 (2006).
Matteo Osellaa, E. N. & Lagomarsino, M. C. Concerted control of Escherichia coli cell division. Proc. Natl. Acad. Sci. USA 111, 3431–3435. https://doi.org/10.1073/pnas.1313715111 (2014).
Yang, D. C., Blair, K. M. & Salama, N. R. Staying in shape: the impact of cell shape on bacterial survival in diverse environments. Microbiol. Mol. Biol. Rev. 80, 187–203. https://doi.org/10.1128/mmbr.00031-15 (2016).
Corey, S. & Westfall, P. A. L. Bacterial cell size: multifactorial and multifaceted. Annu. Rev. Microbiol. 71, 499–517. https://doi.org/10.1146/annurev-micro-090816093803 (2017).
Zhu, C. et al. Variable cell division time and asymmetric division site lead to filament-to-rod cell cycle of Lysinibacillus varians. FEMS Microbiol. Lett. 367, fnaa054. https://doi.org/10.1093/femsle/fnaa054 (2020).
Cooper, S. & Helmstetter, C. E. Chromosome replication and the division cycle of Escherichia coli B/r. J. Mol. Biol. 31, 519–540 (1968).
Donachie, W. D. Relationship between cell size and time of initiation of DNA replication. Nature 219, 1077–1079 (1968).
Campos, M. et al. A constant size extension drives bacterial cell size homeostasis. Cell 159, 1433–1446. https://doi.org/10.1016/j.cell.2014.11.022 (2014).
Taheri-Araghi, S. et al. Cell-size control and homeostasis in bacteria. Curr. Biol. 25, 385–391. https://doi.org/10.1016/j.cub.2014.12.009 (2015).
Harris, L. K. & Theriot, J. A. Relative rates of surface and volume synthesis set bacterial cell size. Cell 165, 1479–1492. https://doi.org/10.1016/j.cell.2016.05.045 (2016).
Hai Zheng, P.-Y.H. et al. Interrogating the Escherichia coli cell cycle by cell dimension perturbations. Proc. Natl. Acad. Sci. USA 113, 15000–15005 (2016).
Thomas Kruse, J.M.-J., Lobner-Olesen, A. & Gerdes, K. dysfunction MreB inhibits chromosome segregation in Escherichia coli. EMBO J. 22, 5283–5292 (2003).
Shi, H. et al. Deep phenotypic mapping of bacterial cytoskeletal mutants reveals physiological robustness to cell size. Curr. Biol. 27, 3419–3429. https://doi.org/10.1016/j.cub.2017.09.065 (2017).
Bean, G. J. et al. A22 disrupts the bacterial actin cytoskeleton by directly binding and inducing a low-affinity state in MreB. Biochemistry 48, 4852–4857. https://doi.org/10.1021/bi900014d (2009).
Awuni, E. & Mu, Y. Effect of A22 on the conformation of bacterial actin MreB. Int. J. Mol. Sci. 20, 1304. https://doi.org/10.3390/ijms20061304 (2019).
Si, F., Li, B., Margolin, W. & Sun, S. X. Bacterial growth and form under mechanical compression. Sci. Rep. 5, 11367. https://doi.org/10.1038/srep11367 (2015).
Caspi, Y. Deformation of filamentous Escherichia coli cells in a microfluidic device: a new technique to study cell mechanics. PLoS ONE 9, e83775. https://doi.org/10.1371/journal.pone.0083775 (2014).
Mannik, J. W. et al. Robustness and accuracy of cell division in Escherichia coli in diverse cell shapes. Proc. Natl. Acad. Sci. USA 109, 6957–6962. https://doi.org/10.1073/pnas.1120854109 (2012).
Wu, F., van Schie, B. G., Keymer, J. E. & Dekker, C. Symmetry and scale orient Min protein patterns in shaped bacterial sculptures. Nat. Nanotechnol. 10, 719–726. https://doi.org/10.1038/nnano.2015.126 (2015).
Thanbichler, M. Synchronization of chromosome dynamics and cell division in bacteria. Cold Spring Harb. Perspect. Biol. 2, a000331. https://doi.org/10.1101/cshperspect.a000331 (2010).
LaBreck, C. J., Conti, J., Viola, M. G. & Camberg, J. L. MinC N- and C-domain interactions modulate FtsZ assembly, division site selection, and MinD-dependent oscillation in Escherichia coli. J. Bacteriol. https://doi.org/10.1128/JB.00374-18 (2019).
Krupka, M. & Margolin, W. Unite to divide: oligomerization of tubulin and actin homologs regulates initiation of bacterial cell division. F1000Res 7, 235. https://doi.org/10.12688/f1000research.13504.1 (2018).
Yang, X. et al. GTPase activity–coupled treadmilling of the bacterial tubulin FtsZ organizes septal cell wall synthesis. Science 355, 744–747 (2017).
Egan, A. J. F. & Vollmer, W. The physiology of bacterial cell division. Ann. N. Y. Acad. Sci. 1277, 8–28. https://doi.org/10.1111/j.1749-6632.2012.06818.x (2013).
Soderstrom, B. et al. Coordinated disassembly of the divisome complex in Escherichia coli. Mol. Microbiol. 101, 425–438. https://doi.org/10.1111/mmi.13400 (2016).
Pichoff, S. & Lutkenhaus, J. Unique and overlapping roles for ZipA and FtsA in septal ring assembly in Escherichia coli. EMBO J. 21, 685–693 (2002).
Rowlett, V. W. & Margolin, W. 3D-SIM super-resolution of FtsZ and its membrane tethers in Escherichia coli cells. Biophys. J. 107, L17–L20. https://doi.org/10.1016/j.bpj.2014.08.024 (2014).
Coltharp, C. & Xiao, J. Beyond force generation: Why is a dynamic ring of FtsZ polymers essential for bacterial cytokinesis?. BioEssays 39, 1–11. https://doi.org/10.1002/bies.201600179 (2017).
Soderstrom, B., Badrutdinov, A., Chan, H. & Skoglund, U. Cell shape-independent FtsZ dynamics in synthetically remodeled bacterial cells. Nat. Commun. 9, 4323. https://doi.org/10.1038/s41467-018-06887-7 (2018).
Wettmann, L., Bonny, M. & Kruse, K. Effects of geometry and topography on Min-protein dynamics. PLoS ONE 13, e0203050. https://doi.org/10.1371/journal.pone.0203050 (2018).
Zaritsky, A., Rabinovitch, A., Liu, C. & Woldringh, C. L. Does the eclipse limit bacterial nucleoid complexity and cell width?. Synth. Syst. Biotechnol. 2, 267–275. https://doi.org/10.1016/j.synbio.2017.11.004 (2017).
Zaritsky, A. & Pritchard, R. H. Changes in cell size and shape associated with changes in the replication time of the chromosome of Escherichia coli. J. Bacteriol. 142, 824–837 (1973).
Coltharp, C., Buss, J., Plumer, T. M. & Xiao, J. Defining the rate-limiting processes of bacterial cytokinesis. Proc. Natl. Acad. Sci. USA 113, 1044–1053 (2016).
Wang, P. et al. Robust growth of Escherichia coli. Curr. Biol. 20, 1099–1103. https://doi.org/10.1016/j.cub.2010.04.045 (2010).
Takeuchi, S., DiLuzio, W. R., Weibel, D. B. & Whitesides, G. M. Controlling the shape of filamentous cells of Escherichia Coli. Nano Lett. 5, 1819–1823 (2005).
Erickson, H. P., Anderson, D. E. & Osawa, M. FtsZ in bacterial cytokinesis: cytoskeleton and force generator all in one. Microbiol. Mol. Biol. Rev. 74, 504–528. https://doi.org/10.1128/MMBR.00021-10 (2010).
Cho, H., McManus, H. R., Dove, S. L. & Bernhardt, T. G. Nucleoid occlusion factor SlmA is a DNA-activated FtsZ polymerization antagonist. Proc. Natl. Acad. Sci. USA 108, 3773–3778. https://doi.org/10.1073/pnas.1018674108 (2011).
Adams, D. W., Wu, L. J. & Errington, J. Nucleoid occlusion protein Noc recruits DNA to the bacterial cell membrane. EMBO J. 34, 491–501. https://doi.org/10.15252/embj.201490177 (2015).
Renner, L. D., Eswaramoorthy, P., Ramamurthi, K. S. & Weibel, D. B. Studying biomolecule localization by engineering bacterial cell wall curvature. PLoS ONE 8, e84143. https://doi.org/10.1371/journal.pone.0084143 (2013).
Ursell, T. S. et al. Rod-like bacterial shape is maintained by feedback between cell curvature and cytoskeletal localization. Proc. Natl. Acad. Sci. USA 111, E1025-1034. https://doi.org/10.1073/pnas.1317174111 (2014).
Buss, J. et al. A multi-layered protein network stabilizes the Escherichia coli FtsZ-ring and modulates constriction dynamics. PLoS Genet. 11, e1005128. https://doi.org/10.1371/journal.pgen.1005128 (2015).
Zaritsky, A., Vollmer, W., Mannik, J. & Liu, C. Does the nucleoid determine cell dimensions in Escherichia coli?. Front. Microbiol. 10, 1717. https://doi.org/10.3389/fmicb.2019.01717 (2019).
Jia, S. et al. Effect of the Min system on timing of cell division in Escherichia coli. PLoS ONE 9, e103863. https://doi.org/10.1371/journal.pone.0103863 (2014).
Gabriele Micali, J. G., Osella, M. & Lagomarsino, M. C. Concurrent processes set E. coli cell division. Sci. Adv. 4, 3324 (2018).
Acknowledgements
We are grateful to Mr. Baogang Quan for manufacturing the microchannel chip. And thanks to Dr. Shuyi Wu and Mr. Chuang Li for help setting up the experimental system in our laboratory. This study is supported by National Natural Science Foundation of China (Grant No.11674387, 11574385, 115674368), National Key R&D Program of China (Grant No. 2016YFA0200800).
Author information
Authors and Affiliations
Contributions
B.L. and L.X. designed the research. C.L. and M.F. helped construct the strain. B.Q. and L.J. helped fabricate the chip. B.H. performed the research. B.L. analyzed the data. B.L. L.X. and A.B.L wrote the paper.
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing interests.
Additional information
Publisher's note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary information
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Liang, B., Quan, B., Li, J. et al. Artificial modulation of cell width significantly affects the division time of Escherichia coli. Sci Rep 10, 17847 (2020). https://doi.org/10.1038/s41598-020-74778-3
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41598-020-74778-3
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.