Expression of the Human Herpesvirus 6A Latency-Associated Transcript U94A Disrupts Human Oligodendrocyte Progenitor Migration

Progression of demyelinating diseases is caused by an imbalance of two opposing processes: persistent destruction of myelin and myelin repair by differentiating oligodendrocyte progenitor cells (OPCs). Repair that cannot keep pace with destruction results in progressive loss of myelin. Viral infections have long been suspected to be involved in these processes but their specific role remains elusive. Here we describe a novel mechanism by which HHV-6A, a member of the human herpesvirus family, may contribute to inadequate myelin repair after injury.


Results
We previously showed that infection of hOPCs with whole virions of HHV-6A show syncytia formation, intercellular production of viral particles, cell cycle arrest and premature differentiation 13 . While it was at this time not known that HHV-6A establishes latency via genome integration, we noticed in these earlier studies that HHV-6A infected hOPCs did not die and the cell cycle arrest seemed transient, suggesting a possible progression from an abortive infection with no cytotoxic effects to a latency state. To test whether acutely infected hOPCs progress to such a latency stage and express the latency associated viral transcript U94A, we exposed hOPCs 14 (Fig. 1) to cell-free, fluorescently labeled HHV-6A virions 13 and, using reverse transcriptase PCR, detected robust expression of U94A at 4 and 10 days post-infection (Fig. 2a). As the cell cycle arrest that occurs after acute infection prevented us from generating large numbers of hOPCs that would express the latency transcript U94A, we directly expressed the latency gene in hOPCs using a lentiviral vector co-expressing red fluorescent protein (RFP). We then analyzed cell survival, migration, proliferation and differentiation, all of which are critical OPC functions during remyelination. hOPCs expressing only green fluorescent protein (GFP) served as controls. The expression of different fluorescent proteins in control versus U94A+ cells allowed us quantify the cellular readouts in experiments where we mixed the two populations to ensure exposure to identical conditions.
We found an intriguing impairment of in vitro migration in U94A-expressing hOPCs using an agarose drop assay. As quantified in (Fig. 2b) the number of U94A RFP+ hOPCs that migrated from the drop was significantly reduced compared to GFP+ control cells migrating from the same drop (p < 0.0001, unpaired t-test). We were concerned that the decrease in U94A+ cells that migrated out of the agarose drop could have been due to a decrease in cell survival of U94A+ cells. However, we did not see a significant change in the number of live cells of U94A+ versus control cultures (Fig. 2c) (p = 0.518, 2-way ANOVA). To confirm that the cell number data represent survival, rather than increased proliferation coupled with increased cell death, we labeled cells with BrdU and analyzed incorporation after 4, 24, and 48 hours (Fig. 2d). We did not find a significant difference in proportions of dividing cells, suggesting that the impaired migration of U94A+ hOPCs was not due to impaired survival or proliferation. To determine whether U94A expression affects differentiation, we induced cells with bone morphogenetic protein 4 (astrocyte induction) or thyroid hormone (oligodendrocyte induction). We found no significant differences in the number of astrocytes (Fig. 2e) or oligodendrocytes generated (Fig. 2f).  [19][20][21][22] week fetal human tissue by immunomagnetic purification using anti-CD140 beads. When cultured in the presence of PDGF and FGF for 7 days, these cells expressed a variety of known OPC markers, including (a) PDGFRα, (b) Nkx2.2, (c) CNPase, (d) A2B5, (e) NG2, and (f) PSA-NCAM. (g) When exposed to bone morphogenic protein 4 (BMP-4) for 7 days, cells differentiated into GFAP+ astrocytes. (h) When exposed to triiodothyronine/thyroxine (T 3 /T 4 ) for 10 days, these cells differentiated into GalC+ oligodendrocytes.
Scientific RepoRts | 7: 3978 | DOI:10.1038/s41598-017-04432-y As impairment of hOPC migration leads to poor remyelination 4 , we wanted to confirm that the in vitro data are relevant in the complex in vivo environment of demyelinated tissue. To create a consistent injury with extensive myelin loss, we treated immunocompromised NSG mice with cuprizone for 4 weeks, leading to robust demyelination 15 . We then stereotactically transplanted U94A RFP+ and GFP+ control hOPCs contralateral into the corpus callosum and hippocampus (Fig. 3a). Twenty-one days after hOPC transplantation, brains were harvested and analyzed. Consistent with our in vitro studies, we saw significant impairment in the migration of the U94A RFP+ cell pool compared to the control GFP+ cells in both the hippocampus (Fig. 3b) and the corpus callosum (Fig. 3c). Most U94A RFP+ cells remained as a bolus, while GFP+ cells dispersed widely throughout both white and gray matter (Fig. 3b,c). Quantification of cell spread of the U94A RFP+ and GFP+ hOPCs (Fig. 3d) showed a significant difference in their migration after transplantation of equal numbers of cells into each brain (p = 0.0178, unpaired t-test).
We also examined whether U94A expression altered the differentiation potential of transplanted hOPCs in vivo. We found that both U94A RFP+ cells and GFP+ controls generated GST-pi+ oligodendrocyte progeny (Fig. 3e) while neither of the cell populations were found to contain GFAP+ astrocytes (Fig. 3f). This finding is to be expected, as previous murine transplant studies have demonstrated that even at 8 weeks post injection, fewer than 5% of human cells express GFAP 14 . While > 90% of the GFP+ cells expressed GST-pi, we could not clearly quantify the number of U94A RFP+ cells that also stained for GST-pi, due to the impaired migration and clumping of the cells at the injection site.

Discussion
In conclusion, (i) infection of hOPCs with HHV-6A is associated with the expression of the latency viral protein U94A and (ii) expression of the latency-associated viral protein U94A is sufficient to decrease the migration of hOPCs in vitro as well as in vivo. Our data raise the possibility that CNS viral latency is not a benign state, but can contribute to defective myelin repair through impaired recruitment of myelinating OPCs after injury. These data suggest, for the first time, a potential contribution of latent HHV-6A infection in the progression of demyelinating diseases.
This view offers a significant paradigm shift from previous approaches that focused on the effects of primary HHV-6A infection on CNS cell types and which have attempted to link the presence of HHV-6A with disease initiation 16 . Our hypothesis that HHV-6A latency may cause inefficient OPC-mediated repair in patients with (e,f) U94A RFP expression does not significantly affect OPC differentiation into GFAP+ astrocytes or GalC+ oligodendrocytes in vitro. Data from 3-6 independent experiments, normalized to GFP+ controls, pooled and displayed as mean ± standard error of the mean (SEM); ns = not significant; ***p < 0.001 versus control. Effects of U94A expression on hOPC proliferation and BrdU incorporation were analyzed by two-way ANOVA. Effects of U94A expression on hOPC migration and differentiation were analyzed by unpaired student's t-test. Nested rtPCR agarose gel shown as cropped image. Full images shown in supplementary information. chronic demyelinating disease is consistent with both evidence of HHV-6 viral infection but an absence of infectious virions in demyelinated lesions 12,17 and a failure of OPCs to populate these lesion sites 4,5 . We therefore propose that the expression of viral transcripts and proteins during HHV-6A latency may contribute to progression of chronic demyelinating diseases. Lastly, our findings have important implications for the use of hOPCs for therapeutic purposes and the need to screen for and exclude the presence of HHV-6 in cell transplants.

Methods
Human Oligodendrocyte Progenitor (hOPC) Isolation. Human OPCs were isolated and maintained as previously described 18 . Briefly, cortical tissue from 19-21 week old fetal brain was dissected and the minced tissue was digested at 37 °C with 59 U/ml papain  virus preparation was added to hOPCs suspended in 800 ul of DMEM:F12 complete media supplemented with 10 ng/mL PDGF-AA and basic FGF. Following a four hour incubation at 37 °C (5% O 2 /7% CO 2 ), the cells were replated in DMEM:F12 complete media supplemented with 10 ng/mL PDGF-AA and basic FGF. At four and ten days post infection, RNA was isolated from the hOPCs using a Nucleospin RNA kit (Machery-Nagel #740955) as per manufacturer's protocol. 70 ng of RNA from each sample were converted to cDNA using an iScript cDNA Synthesis kit (Bio-Rad #1708890) with the addition of RNasin Plus RNase inhibitor (Promega N261B). For PCR analysis of U94A transcript expression, 5 μL of each cDNA sample was transferred to 50 μL nested PCR reactions as previously described 19, 20 . U94A Viral Propagation and hOPC Infection. HHV-6A cDNA was generated as above, and the U94A transcript was amplified and ligated into a RFP+ puromycin lentivector (System Biosciences #CD516B-1). Empty GFP lentivector (System Biosciences #CD513B-1) was used as a control. We used different fluorescent proteins to be able to clearly distinguish U94A+ cells from control cells. For viral production, 293 T cells were propagated in DMEM (Gibco #11965-02) with 10% fetal calf serum. Following co-transfection with Pax2 and VSV-G expressing plasmids, 293 T cells were maintained in 1% fetal calf serum for 72 h before harvesting viral supernatant. Infections of hOPCs were performed by adding lentiviral supernatant to cell cultures at a dilution of 1:4 in DMEM:F12 complete media supplemented with 10 ng/mL PDGF-AA and basic FGF. Cells were incubated at 37 °C (5% O 2 /7% CO 2 ) for 4 h before media was changed.
Analysis of hOPC Proliferation. The replication rate of the cells was determined by plating hOPCs on poly-L-lysine coated 96-well plates at a density of (300) cells/cm 2 in DMEM:F12 complete media with 10 ng/mL PDGF-AA and basic FGF. The total number of hOPCs per well was determined using Brightfield analysis with a Celigo cytometer (Nexcelom) every other day across 21 days, and cell counts were normalized to the number of cells at the beginning of the experiment (Day 1).
In Vitro hOPC Migration. Cell migration with agarose drops was performed as previously reported (Milner et al., 1997, Glia, p85-90) Briefly, U94A RFP or control GFP lentiviral infected hOPCs were mixed to equal parts, resuspended in 0.3% low-melt agarose (at 37 °C; Sigma #A0701), and diluted in DMEM:F12 complete media supplemented with 10 ng/mL PDGF-AA and basic FGF. at a density of /μL. 1.5 μL (containing 6 x 10 4 cells) of the cell-agarose mixture was plated in the center of a poly-L-lysine-coated 24-well plate. The agarose was allowed to gel at 4 °C for 10 min before DMEM:F12 complete media supplemented with 10 ng/mL PDGF-AA and FGF. The number of U94A RFP+ and control GFP+ cells that had migrated from the edge of the agarose drop was quantified.
In Vitro hOPC Differentiation. Experiments involved first expanding hOPCs in 10 ng/ml PDGF-AA and basic FGF growth factor (bFGF). At passage, cells were plated for experiments on poly-L-lysine coated glass coverslips at a density of 1500 cells/cm 2 in DMEM:F12 complete media with 10 ng/mL PDGF-AA and basic FGF and allowed 24 h for recovery before treatment. Differentiation conditions consisted of 1 ng/ml PDGF-AA plus 40 nm thyroid hormone (TH) (30 ng/ml thyroxine and 36 ng/ml triiodothrionine) for 10 days to induce oligodendrocytes differentiation or 10 ng/ml bone morphogenetic protein 4 (BMP-4, R&D Systems #314-BP) for 7 days to induce astrocytes differentiation.
Cuprizone Model and hOPC Transplants. All animal procedures were performed under guidelines of the National Institutes of Health and approved by the Institutional Animal Care and Utilization Committee (IACUC) of the University of Rochester Medical Center, Rochester, NY. Six month old male NSG mice were obtained from in-house breeding and fed with a diet of chow mixed with 0.2% cuprizone over the course of four weeks. Animal weights were recorded three times per week and care for those that lost > 15% body weight was discussed with a veterinarian. Animals showing inability to ambulate, inability to maintain food or water intake, and clinical signs of pain including ruffled fur, hunched posture, vocalization and guarding behavior were acutely euthanized. Handling of the animals when they were weighed was kept to a minimum and all animals were group housed to avoid isolation stress. For the transplantation procedure, mice were anesthetized with ketamine (100 mg/kg, i.p.) and xylazine (10 mg/kg, i.p.), and secured in a stereotactic frame (David Kopf Instruments, Tujunga, CA, USA). Six burr holes were drilled at 3 coordinates in each hemisphere (Bregma: mediolateral 1.5mm, anterior-posterior 0, 1.0, 2.0 mm, dorsoventral 0.9 mm). U94A RFP or control GFP-expressing hOPCs were resuspended in PBS at a concentration of 25,000 cells/μl. Two μl of U94A RFP or GFP cells were injected in contralateral hemispheres with a ten-μl syringe fitted with a glass capillary injection needle (80 µm diameter, beveled tip; pulled using a P1000 micropipette puller, Sutter Instruments).
Immunohistochemistry. 21 days following stereotactic cell injection, mice were transcardially perfused with 4% paraformaldehyde/PBS. Brains were isolated and post-fixed for 24 h in 4% paraformaldehyde and normalized for 48 h in 20% sucrose. Brains were frozen with dry ice and sectioned at 25-μm thickness using a cryotome. The sections underwent three washes in PBST (phosphate buffered saline + 0.03% Triton X-100) and were blocked in 5% fetal bovine serum serum in PBST (1 h). Sections were then immunostained overnight at 4 °C with GFAP (1:300; Sigma #G3893), and GSTpi (1:500; BD Biosciences #610718). The next day, the tissue was washed three times in PBST and incubated for 1 h in appropriate Alexa-Fluor-conjugated secondary at a concentration of 1 μg/ml (Thermo Fisher Scientific) and counter-stained with 1 μg/ml 4′6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific #D1306). Stained sections were rinsed three times in 1 × PBS and mounted on glass slides with Fluoromount-G (SouthernBiotech #0100-01). Multi-channel fluorescence mosaic images were acquired by LAS AF software using a Leica TCS SP5 laser confocal microscope (Leica Microsystems, Mannheim, Germany). Images were acquired with a 40 × oil immersion lens (Leica) and z-projections of 6 images taken at 7-μm step intervals.

Cell Spread Measurements.
Migration data represent analyses of the corpus callosa and hippocampi of four animals. A minimum of three sections per animal were analyzed, and only sections containing both GFP+ and U94A RFP+ cells were used for analysis. To measure cell spread, coordinate positions of each cell were determined and the distance of each cell from the mean coordinate position of the cell population was measured using LAS AF software (Leica Microsystems, Mannheim, Germany).
Statistics. Bar graphs are plotted as mean ± SEM and represent, at minimum, three independent biological replicates performed in triplicate. Two-group comparisons were analyzed using a Student's t test, and multiple-timepoint comparisons were analyzed by two-way ANOVA. Prism (v5.0; GraphPad) was used for data analysis and presentation.