Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

Microscopy

IMAGE-seq and you shall find

Nature Methods volume 20, pages 639–640 (2023)Cite this article

Subjects

Integration of single-cell molecular profiling with cellular spatial localization has remained an elusive goal. Image-seq leverages high-resolution microscopy to spatially resolve and isolate viable bone marrow and leukemia cells for subsequent state-of-the art, single-cell transcriptomics.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Fig. 1: Image-Seq.

References

  1. Jaitin, D. A. et al. Science 343, 776–779 (2014).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Pollen, A. A. et al. Nat. Biotechnol. 32, 1053–1058 (2014).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Haase, C. et al. Nat. Methods https://doi.org/10.1038/s41592-022-01673-2 (2022).

    Article  PubMed  PubMed Central  Google Scholar 

  4. Rodrigues, S. G. et al. Science 363, 1463–1467 (2019).

    Article  Google Scholar 

  5. Eng, C.-H. L. et al. Nature 568, 235–239 (2019).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Chen, K. et al. Science 348, 6090 (2015).

    Article  Google Scholar 

  7. Moffitt, J. R. et al. Proc. Natl Acad. Sci. USA 113, 14456–14461 (2016).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Trapnell, C. et al. Nat. Biotechnol. 32, 381–386 (2014).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Medaglia, C. et al. Science 358, 1622–1626 (2017).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Love, M. I., Huber, W. & Ander, S. Genome Biol. 15, 550 (2014).

    Article  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

The authors thank Bryanna C. Reinhardt, Louisiana State University School of Medicine, for illustration.

Author information

Authors and Affiliations

  1. Division of Hematology & Cellular Therapy, Department of Medicine, Cedars Sinai Medical Center, Los Angeles, CA, USA

    John P. Chute & Joshua P. Sasine

  2. Regenerative Medicine Institute, Cedars Sinai Medical Center, Los Angeles, CA, USA

    John P. Chute & Joshua P. Sasine

  3. Samuel Oschin Cancer Institute, Cedars Sinai Medical Center, Los Angeles, CA, USA

    John P. Chute & Joshua P. Sasine

Authors
  1. John P. Chute
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Joshua P. Sasine
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to John P. Chute.

Ethics declarations

Competing interests

The authors declare no competing interests.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Chute, J.P., Sasine, J.P. IMAGE-seq and you shall find. Nat Methods 20, 639–640 (2023). https://doi.org/10.1038/s41592-023-01835-w

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41592-023-01835-w

Search

Advanced search

Quick links

Nature Briefing: Cancer

Sign up for the Nature Briefing: Cancer newsletter — what matters in cancer research, free to your inbox weekly.

Get what matters in cancer research, free to your inbox weekly. Sign up for Nature Briefing: Cancer