Correction to: Nature Methods https://doi.org/10.1038/s41592-020-0858-0, published online 15 June 2020.

The version of this article initially published did not include the probe design software in the Code Availability statement. The statement has been updated to note the availability of probe design software at https://github.com/khchenLab/split-fish. In addition, as a result of a publisher error, the source data for Fig. 1 were provided as .xls files. These have been corrected to .tif files. The errors have been corrected in the HTML and PDF versions of the article.