A multimodal imaging approach is developed to interrogate microorganism–semiconductor biohybrids at the single-cell and single-molecule level for light-driven CO2 fixation. Application to lithoautotrophic bacterium Ralstonia eutropha biohybrids reveals the roles of two hydrogenases in electron transport and bioplastic formation, the magnitude of semiconductor-to-single-cell electron transport and the associated pathways.
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References
Kornienko, N., Zhang, J. Z., Sakimoto, K. K., Yang, P. & Reisner, E. Interfacing nature’s catalytic machinery with synthetic materials for semi-artificial photosynthesis. Nat. Nanotechnol. 13, 890–899 (2018). A review article that summarizes progress in enzymatic and microbial hybrids for solar-to-fuel conversion.
Sakimoto, K. K., Wong, A. B. & Yang, P. Self-photosensitization of nonphotosynthetic bacteria for solar-to-chemical production.Science 351, 74–77 (2016). This paper reports that nanoparticles can capture light to enable a non-photosynthetic bacterium to produce acetic acid from CO2.
Liu, C., Colón, B. C., Ziesack, M., Silver, P. A. & Nocera, D. G. Water splitting-biosynthetic system with CO2 reduction efficiencies exceeding photosynthesis. Science 352, 1210–1213 (2016). This paper reports that a biocompatible catalyst in an artificial photosynthesis system can lead to efficient solar-to-fuel conversion.
Fu, B. et al. Metal-induced sensor mobilization turns on affinity to activate regulator for metal detoxification in live bacteria. Proc. Natl Acad. Sci. USA 117, 13248–13255 (2020). This paper reports a single-molecule imaging study of a two-component regulatory system in bacteria.
Sambur, J. B. et al. Sub-particle reaction and photocurrent mapping to optimize catalyst-modified photoanodes. Nature 530, 77–80 (2016). This paper reports single-molecule, super-resolution reaction imaging of photoelectrocatalysis on single semiconductor nanostructures.
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This is a summary of: Fu, B. et al. Single-cell multimodal imaging uncovers energy conversion pathways in biohybrids Nat. Chem. https://doi.org/10.1038/s41557-023-01285-z (2023).
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A multimodal imaging approach for interrogating energy-conversion pathways in biohybrids. Nat. Chem. 15, 1336–1337 (2023). https://doi.org/10.1038/s41557-023-01286-y
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DOI: https://doi.org/10.1038/s41557-023-01286-y
