Abstract
Worldwide, people are getting older, and this prolonged lifespan unfortunately also results in an increased prevalence of age-related neurodegenerative diseases, contributing to a diminished life quality of elderly. Age-associated neuropathies typically include diseases leading to dementia (Alzheimer’s and Parkinson’s disease), as well as eye diseases such as glaucoma and age-related macular degeneration. Despite many research attempts aiming to unravel aging processes and their involvement in neurodegeneration and functional decline, achieving healthy brain aging remains a challenge. The African turquoise killifish (Nothobranchius furzeri) is the shortest-lived reported vertebrate that can be bred in captivity and displays many of the aging hallmarks that have been described for human aging, which makes it a very promising biogerontology model. As vision decline is an important hallmark of aging as well as a manifestation of many neurodegenerative diseases, we performed a comprehensive characterization of this fish’s aging visual system. Our work reveals several aging hallmarks in the killifish retina and brain that eventually result in a diminished visual performance. Moreover, we found evidence for the occurrence of neurodegenerative events in the old killifish retina. Altogether, we introduce the visual system of the fast-aging killifish as a valuable model to understand the cellular and molecular mechanisms underlying aging in the vertebrate central nervous system. These findings put forward the killifish for target validation as well as drug discovery for rejuvenating or neuroprotective therapies ensuring healthy aging.
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Introduction
Improved living conditions and advances in medicine have led to the rise in longevity that is seen today. Human populations are becoming older allover western civilizations and by 2050, one in six people will be 65 years or older. However, this observed prolonged lifespan is not necessarily accompanied by an increased health span. On the contrary, a higher prevalence of age-related central nervous system (CNS) diseases is observed in our graying society. Worldwide, 50 million people live with dementia (mostly Alzheimer’s and Parkinson’s disease) and this number doubles every 5 years1,2,3. Aging also often goes together with a decline in vision, which greatly impacts the ability to carry out everyday tasks. Indeed, for nonneurodegenerative eye conditions, including dry eye and cataract, but importantly also for neurodegenerative eye diseases, such as glaucoma and age-related macular degeneration, age is a major risk factor. Unfortunately, despite the global high incidence of neurodegenerative eye pathologies, treatments that can halt or reverse these disorders are nonexistent. Understanding the aging context seems to be crucial for finding curative therapies, as age clearly affects the integrity and functionality of the visual system, and therefore also its already limited ability to protect or repair damaged/degenerating neurons4,5,6.
Aging is defined by numerous alterations at a cellular and molecular level that eventually result in age-related deterioration of physiological functions7. In the brain, ten hallmarks were established: (1) mitochondrial dysfunction; (2) intracellular accumulation of oxidatively damaged proteins, nucleic acids, and lipids; (3) dysregulated energy metabolism; (4) impaired cellular waste disposal mechanisms; (5) impaired adaptive stress response signaling; (6) compromised DNA repair; (7) aberrant neuronal network activity; (8) dysregulated neuronal Ca2+ handling; (9) stem cell exhaustion; and (10) inflammation. Note that the manifestation of cellular senescence and telomere attrition, two hallmarks described to occur in proliferative peripheral tissues, was not convincingly proven in neurons but does occur in glial cells of the CNS7,8,9.
While many fish species, such as zebrafish and goldfish, have been exploited in biogerontology research, their relatively long lifespan complicates investigating aging in these animal models. With its relatively short lifecycle compared to other vertebrate models and by displaying several aging hallmarks that have been described for humans7, the African turquoise killifish (Nothobranchius furzeri) has been put forward as an excellent aging model to fill this gap10,11,12. Furthermore, although not yet extensively studied, its CNS has been shown to be subject to aging too, with indications for the occurrence of typical age-related hallmarks including lipofuscin accumulation, impaired protein homeostasis, mitochondrial dysfunction, reactive gliosis, and reduced adult neurogenesis, which eventually lead to impairments in locomotor, learning, and memory function13,14,15,16,17,18. The GRZ-AD inbred strain, in particular, is the shortest-lived of all strains, with a median lifespan of 4–6 months19,20,21. Using this model organism, age-associated changes as well as the underlying cellular and molecular processes can be investigated in depth, in order to understand and more efficiently tackle neurodegenerative events.
Over the years, the visual system has become a powerful tool to investigate the brain as a whole, both in mammals and in fish22. The retina is an integral part of the CNS, and disease processes occurring in the retina have shown to be an indicator of similar processes occurring elsewhere in the CNS, and vice versa. Indeed, retinal manifestations of age-related degenerative diseases, such as Alzheimer’s and Parkinson’s disease, have been documented23,24,25,26 and, as such, the eye is often named “a window to the brain.” Yet, the aging processes of the retinotectal system of the killifish, comprising of the retina, optic nerve, optic tract, and optic tectum, has not been characterized. Therefore, in this paper, we rigorously analyzed several age-associated processes in the retina and optic tectum of four different age groups: young adult, middle-aged, old, and very old fish. Our results reveal indications for oxidative stress, cellular senescence, reactive gliosis, inflammaging, declined neurogenic potential, and neurodegeneration in the aged killifish visual system, all together leading to age-related vision impairment. Importantly, the occurrence of these aging hallmarks and the functional decline in the retinotectal system demonstrates its value to study the cellular and molecular mechanisms underpinning brain aging, and could help elucidating physiological versus pathological aging. All in all, we propose the killifish visual system as a model to not only investigate aging processes as such, but also for the identification of new strategies for regenerative medicine, CNS rejuvenation, and healthy aging.
Results
Killifish growth rate decreases with age
In contrast to mammals, fish keep on growing throughout their adult life27. To quantify this growth and evaluate whether the growth rate of GRZ-AD killifish changes with age, the absolute body length, retinal perimeter, retinal surface, tectal perimeter, and tectal surface of 6-week-, 12-week-, 18-week-, and 24-week-old female fish, representing young adult, middle-aged, old, and very old fish, respectively, were measured and the growth rate of these parameters was calculated (Fig. 1a–e). All tested parameters showed a similar trajectory, i.e., growth rate decreased with advancing age, as has been described for both zebrafish and killifish body size16,28, and for zebrafish retina29. A high growth rate was observed in 6-week-old fish, with an increase of 31.6 ± 1.1% in body length, 27.2 ± 2.2% in retinal perimeter, 57.9 ± 2.6% in retinal surface, 26.4 ± 2.5% in tectal perimeter, and 40.1 ± 3.6% in tectal surface between 6 weeks and 12 weeks of age. Between 12 weeks and 18 weeks of age, body length only increased with 9.9 ± 1.0%, retinal perimeter with 14.3 ± 2.6%, retinal surface with 24.2 ± 3.5%, tectal perimeter with 12.6 ± 2.5%, and tectal surface with 31.2 ± 5.0%. Growth was even more confined in fish between 18 weeks and 24 weeks old (5.4 ± 0.8% in body length, 8.8 ± 2.5% in retinal perimeter, 8.4 ± 5.3% in retinal surface, 1.50 ± 2.5% in tectal perimeter, and 2.3 ± 2.5% in tectal surface). Altogether, our results reveal a rapid growth until the age of 12 weeks, which declines between 12 weeks and 18 weeks. After 18 weeks, killifish hardly grow.
a Growth rate of body length in 6-week-, 12-week-, 18-week-, and 24-week-old fish significantly decreases in all age groups. n ≥ 56. b Growth rate of the retinal perimeter, analyzed on mid-sagittal cryosections, significantly declines between 6 weeks and 12 weeks. Thereafter, growth rate does not reduce any further. n ≥ 11. c Also the growth rate of the retinal surface, determined on wholemounts, shows a significant decrease between the age of 6 weeks and 12 weeks. n = 5. d Growth rate of the tectal perimeter, analyzed on coronal cryosections of the central optic tectum, declines upon aging, with a significant reduction between the age of 6 weeks and 12 weeks, and between 18 weeks and 24 weeks. n ≥ 9. e Similar to the tectal perimeter, assessing growth rate of the tectal surface demonstrates a significant drop in growth rate between 6 weeks and 12 weeks, and 18 weeks and 24 weeks of age. n ≥ 8. All data are depicted as mean ± standard error of mean, statistical significance between different age groups is indicated using different letters.
Molecular and cellular changes in the aged killifish visual system
During brain aging, numerous age-associated cellular, molecular, and systemic alterations, described as “hallmarks of aging”, lead to decreased motor, sensory, and cognitive function in elderly8. To characterize aging of the female killifish retinotectal system, we investigated the manifestation of several hallmarks in both the retina and optic tectum of the four age groups, i.e., oxidative stress, cellular senescence, reactive gliosis, inflammaging and stem cell exhaustion, as well as the functional phenotypes related to these changes.
To assess the presence of oxidative stress in both the aged retina and optic tectum, levels of 4-hydroxynonenal (4-HNE), a byproduct of lipid peroxidation and a typical marker for oxidative damage30,31,32, were quantified using western blotting (Fig. 2a). With increasing age, 4-HNE levels tended to elevate in the retina (Fig. 2b), and significantly increased in the tectum between 6-week- and 18-week- or 24-week-old animals (Fig. 2c). In addition, immunohistochemistry for heme oxygenase 1 (Ho-1), a protective antioxidant of which the expression is typically induced in response to oxidative stress33,34, was performed on retinal cryosections (Fig. 2d). Quantification of the immunopositive area within a predefined region of the central retina revealed a significant elevation of Ho-1 expression with advancing age, with a most prominent increase in the inner nuclear layer (INL) of the retina (Fig. 2e). Altogether, these findings are indicative for increased oxidative stress in the aged female killifish visual system.
a Representative western blot images revealing 4-HNE in the retina and optic tectum of fish of the various age groups. Quantitative analysis discloses a tendency to increased levels in the retina (b), and a significant age-related increase of 4-HNE levels in the optic tectum of fish of 18 weeks and 24 weeks old (c). All blots derive from the same experiment and were processed in parallel. Full-length blots are presented in Supplementary Fig. 3. n = 3. d Microscopic pictures of Ho-1-labeled retinal cryosections show an elevated expression of this enzyme with increasing age, especially visible in the INL. Scale bar = 100 µm. e Quantification of the area occupied by the Ho-1-positive signal in the retina, expressed relative to its measured surface area, reveals a significant age-associated upregulation in the killifish retina. n = 5. All values are shown as mean ± standard error of mean, statistical significance between different conditions is depicted using different letters. 4-HNE 4-hydroxynonenal, Ho-1 heme oxygenase 1, GCL ganglion cell layer, IPL inner plexiform layer, INL inner nuclear layer, OPL outer plexiform layer, ONL outer nuclear layer.
The occurrence of cellular senescence in the killifish visual system was evaluated using several known senescence-associated markers. Immunohistochemistry for gamma H2A histone family member X (γH2AX; phosphorylated at the C-terminal serine-139 of the H2AX histone), an indicator of double-stranded DNA breaks that accumulate in senescent cells, on both retinal and tectal cryosections revealed a clear accumulation of DNA damage in aged killifish, especially visible in the nuclei located in the ganglion cell layer (GCL) and INL of the retina, and in the periventricular gray zone (PGZ) of the optic tectum (Fig. 3a). Quantification of the immunopositive area within a predetermined region of the retinal INL and tectal PGZ showed a significant increase in γH2AX-positive nuclei in these nuclear layers (Fig. 3b, c). Cellular senescence was further investigated using mRNA expression studies of cell cycle inhibitors p21 and p27. In the retina as well as in the optic tectum, both p21 and p27 expression levels elevated with age and were significantly higher at 18 weeks compared to 6 weeks. After 18 weeks of age, levels did not increase any further (Fig. 3d, e). Finally, we assessed the expression of lysosomal senescence-associated β-galactosidase (SA-βgal), a putative marker of cellular senescence in peripheral proliferative tissues but also reported in the CNS7,8,9. Staining for SA-βgal on both retinal and tectal cryosections and subsequent quantitative analysis revealed a clear elevation in SA-βgal expression all over the retina, optic nerve, and in the optic tectum in killifish upon aging (Fig. 4a–c). Of note, the observed cellular staining in the optic nerve is indicative of senescence of glial cells, given that only axons and glia are present here. Together, these data suggest a rise in cellular senescence within the female killifish visual system with advancing age, especially manifesting between the age of 12 weeks and 18 weeks.
a Microscopic images of γH2AX-labeled retinal and brain cryosections show a clear accumulation of DNA damage in the nuclei, particularly visible in the GCL and INL of the retina, and the PGZ of the optic tectum. Scale bar = 100 µm. b, c Quantification of the γH2AX-immunopositive area within a predefined region of the retinal INL and tectal PGZ reveals a significant age-related increase. Values represent mean ± standard error of mean, n = 5. d, e Real-time quantitative PCR for cyclin-dependent kinase inhibitors p21 and p27, both regulators of cell cycle progression and typical markers for cellular senescence, reveals a significant rise in gene expression levels with increasing age, both in the retina (d) and optic tectum (e). Values are mean fold change values relative to values of 6-week-old fish ± standard error of mean, n ≥ 4. Statistical significance between different conditions is shown using different letters. γH2AX gamma H2A histone family member X, DAPI 4′,6-diamidino-2-phenylindole, GCL ganglion cell layer, IPL inner plexiform layer, INL inner nuclear layer, OPL outer plexiform layer, ONL outer nuclear layer, PGZ periventricular gray zone, OT optic tectum.
a Representative images of SA-βgal-stained sections show elevated expression levels of this lysosomal enzyme in the aged retina, optic nerve head, and brain. Whereas sections of 6-week-old fish are only scarcely labeled, increased expression levels are detected from 12 weeks onward. Note that these labeled cells are likely glial cells that are senescent. Scale bar = 100 µm. Quantification of the area occupied by SA-βgal-positive labeling demonstrates an age-associated upregulation in the neuroretina (b) as well as optic tectum (c) of the killifish. Values are shown as mean ± standard error of mean, n ≥ 3, statistical significance between different age groups is indicated using different letters. SA-βgal senescence-associated β-galactosidase, GCL ganglion cell layer, IPL inner plexiform layer, INL inner nuclear layer, OPL outer plexiform layer, ONL outer nuclear layer, PRL photoreceptor segment layer, ONH optic nerve head, PGZ periventricular gray zone, OT optic tectum.
In mammals, astroglial cells are known to morphologically and functionally alter with age, with an upregulation of intermediate filament proteins in Müller glia and astrocyte-like cells located in the retina and brain, respectively35,36. Immunostaining for the intermediate filament vimentin, followed by quantification of the labeled area, revealed a significantly augmented expression in retinal Müller glia (Fig. 5a, b) and brain radial glia (Fig. 5a, c) of older killifish when compared to young adult ones. While the dense labeling of the Müller glia endfeet did not seem to alter, detailed microscopic analysis in the killifish retina indeed disclosed a compact network of vimentin-positive radial processes spanning the entire retina from the nerve fiber layer to the outer plexiform layer, which became more prominent in aged fish (Fig. 5a, b). In addition in the optic tectum, the radial glia appeared reactivated, with fibers running from the neuronal layer of the PGZ to the superficial layers, where diffuse processes project into the stratum griseum centrale, stratum fibrosum et griseum superficiale, and stratum opticum, and finally terminate as endfeet on the pia surface (for a review on astrocyte-like cells in the fish brain, see ref. 37) (Fig. 5a, c). All in all, our data show signs of reactive gliosis in the aged female killifish visual system, already from 12 weeks of age.
a Microscopic pictures of vimentin-labeled sections of the retina (sagittal cryosections) and optic tectum (coronal cryosections) reveal a slightly upregulated expression of vimentin in 12-week-old fish when compared to young adult killifish, and a dramatic increase in fish from 18 weeks and 24 weeks old. Scale bar = 100 µm. b, c Quantification of the vimentin-immunopositive area in the retina and the optic tectum reveals a significantly upregulated expression of vimentin in both the retina and tectum of 12-week-, 18-week-, and 24-week-old fish as compared to young adult killifish. Values represent mean ± standard error of mean, n = 5, statistical significance between different age groups is depicted using different letters. GCL ganglion cell layer, NFL nerve fiber layer, IPL inner plexiform layer, INL inner nuclear layer, OPL outer plexiform layer, ONL outer nuclear layer, PRL photoreceptor segment layer, SO stratum opticum, SFGS stratum fibrosum et griseum superficiale, SGC stratum griseum centrale, PGZ periventricular gray zone.
As also the innate immune cell function in the vertebrate CNS is known to alter over life time, resulting in a chronic low-grade inflammatory status named inflammaging38,39,40,41, we next investigated the occurrence of inflammaging in the killifish visual system using immunohistochemistry for the pan-leukocyte marker L-plastin (Fig. 6a). Upon aging, the morphology of resident microglia/macrophages changed from a ramified toward a more ameboid form, particularly visible in 18-week- and 24-week-old fish (Fig. 6a; magnified boxes). Quantification of the L-plastin-immunopositive area within a predefined region in the retina and tectum revealed that, with advancing age, the area occupied by microglia/macrophages tended to increase in the retina (Fig. 6b) and was significantly elevated in the optic tectum (Fig. 6c). Next, to changes in their cell number and morphology, microglia in the aging vertebrate brain also typically display a senescence-associated secretory phenotype (SASP), with increases in proinflammatory and decreases in anti-inflammatory molecules9,42. Real-time quantitative polymerase chain reaction (RT-qPCR) on samples of the killifish retinotectal system disclosed an age-related reduction in mRNA levels of interleukin 10 (il-10), sirtuin 1 (sirt-1), il-6, and il-1b in the retina as well as tectum. On the contrary, an upregulated expression of il-8 and tumor necrosis factor (tnf) could be observed with advancing age (Fig. 6d, e). Together, these findings indicate the occurrence of inflammaging that is accompanied by a different secretory profile in both the old retina and optic tectum of female killifish.
a Representative images of the retina and the optic tectum immunolabeled for the pan-leukocyte marker L-plastin illustrate a visible increase in microglial/macrophage number with increasing age. Moreover, an age-dependent change in morphology is observed in 18-week- and 24-week-old fish, in which microglia seem to transform from a ramified-like toward ameboid-like form (white arrowheads in optic tectum; magnified in boxes; scale bar = 20 µm). Scale bar = 100 µm. b, c Quantification of the area occupied by immunostained leukocytes over the total analyzed area demonstrates an increase in the microglial/macrophage area fraction with increasing age. Values are shown as mean ± standard error of mean, n ≥ 3. d, e Quantification of mRNA levels of inflammatory molecules, such as il-10, sirt-1, il-6, il-1b, sirt-1, il-8, and tnf, in both retinal and tectal lysates reveals significant changes in the expression profile in the retina as well as optic tectum with advancing age. While the expression values of il-10, sirt-1, il-6, and il-1b decline upon aging, levels of il-8 and tnf increase in older fish. Values represent mean fold change values relative to values of 6-week-old fish ± standard error of mean, n ≥ 4. Statistical significance between different conditions is indicated using different letters. DAPI 4′,6-diamidino-2-phenylindole, GCL ganglion cell layer, NFL nerve fiber layer, IPL inner plexiform layer, INL inner nuclear layer, OPL outer plexiform layer, ONL outer nuclear layer, PRL photoreceptor segment layer, SO stratum opticum, SFGS stratum fibrosum et griseum superficiale, SGC stratum griseum centrale, PGZ periventricular gray zone, OT optic tectum, il-10 interleukin 10, il-1b interleukin 1β, il-6 interleukin 6, sirt-1 sirtuin 1, il-8 interleukin 8, tnf tumor necrosis factor.
Another hallmark of aging is stem cell exhaustion43,44,45,46,47. In the fish visual system, neural stem/progenitor cells (NSPCs) are situated in the ciliary marginal zone of the retina, and the dorsomedial and ventrolateral zones at the edges of the PGZ of the optic tectum. To evaluate age-associated alterations in the number of NSPCs, and whether or not they are proliferating, we labeled cycling NSPCs in these mitotic niches via double stainings for the proliferation marker proliferating cell nuclear antigen (Pcna) and the stem cell marker sex-determining region Y-box 2 (Sox2). The presence of proliferating NSPCs in the ciliary marginal zone was clearly detected in the retina of adult killifish (Fig. 7a). However, with increasing age, a decline in the area occupied by the Sox2-immunopositive NSPCs (Fig. 7b) as well as by the Sox2/Pcna double-labeled proliferating NSPCs (Fig. 7c) could be identified. NSPCs were also detected in the dorsomedial (Fig. 7d) and ventrolateral (Supplementary Fig. 1a) neurogenic zones of the adult tectum. Similar as in the retina, an age-associated decrease in both the NSPC-positive area (Fig. 7e) and its proliferative activity (Fig. 7f) was observed. All in all, these results suggest an age-related decay of the NSPC pool as well as a decline in its mitotic activity, in both retina and optic tectum.
a Microscopic images of the neurogenic zones in the killifish visual system, after double labeling for Sox2 and Pcna, disclose proliferating NSPCs within the ciliary marginal zone (indicated with a white dashed line) of the young adult retina. Scale bar = 50 µm. b, c Quantification of the Sox2- and Pcna-immunopositive areas reveals a significant reduction in both NSPCs (Sox2-positive; dashed outline) (b) and proliferating NSPCs (Sox2 and Pcna double-positive) (c) with increasing age. d Proliferating NSPCs have also been identified in the dorsomedial zone of the optic tectum (depicted as a white dashed line). Scale bar = 50 µm. As a function of age, the area occupied by Sox2-positive NSPCs (dashed outline) (e) as well as the area covering the Sox2 and Pcna double-positive proliferating NSPCs (f) significantly decrease in this zone. Note that immunostaining for Sox2 is known to label a subset of amacrine cells in the mature fish retina and a subpopulation of neurons in the periventricular gray zone of the optic tectum105,106,107. All values represent mean ± standard error of mean, n = 5, statistical significance between different age groups is shown using different letters. DAPI 4′,6-diamidino-2-phenylindole, Sox2 sex-determining region Y-box 2, Pcna proliferating cell nuclear antigen, NSPC neural stem/progenitor cell.
To further investigate the neurogenic potential within the neurogenic zones of the killifish visual system, a 5-bromo-2′-deoxyuridine (BrdU) pulse-chase experiment with a 12-h pulse was performed, and neurogenesis was studied at 2 and 21 days after the second intraperitoneal injection (dpIP). At 2 dpIP, BrdU-positive cells were clearly visible in all mitotic zones, independent of age, indicating that new cells were generated in all age groups (Fig. 8a, e; Supplementary Fig. 1b). However, a significant decline in the number of BrdU-labeled cells could be observed with increasing age, which reinforces that the number of dividing cells in the mitotic niches declines with aging (Fig. 8c, g). Following 21 days of chase, BrdU-labeled postmitotic cells, and thus newborn cells could be visualized in all age groups. Reflecting the continuous growth of the killifish retina and brain, these cells were observed further away from the ciliary marginal zone (toward the central retina), and from the dorsomedial or ventrolateral zone (in the PGZ), where they were visualized at 2 dpIP (Fig. 8b, f; Supplementary Fig. 1b). However, in older killifish, the total number of these newly generated cells was much lower than in young adult killifish, suggesting that less cells were added as a function of age, indicative for a decreased neurogenic potential of the NSPCs (Fig. 8b, f). Furthermore, the distance from the neurogenic niche to the BrdU-positive cells at 21 dpIP was clearly shorter in the retina and tectum of older fish, which further supports that the NSPCs in these neurogenic niches divide less often (Fig. 8d, h). These findings thus show a decline in the number of (cycling) NSPCs as well as in their proliferative activity, in the aged retina as well as optic tectum.
a, e Representative images of sections of all four age groups containing the neurogenic zones of the retinotectal system immunostained for BrdU. At 2 dpIP (following a 12 h pulse), BrdU-labeled cells (white dots) are clustered in the neurogenic zones of the retina and the optic tectum (depicted as a dashed line). Scale bar = 50 µm. c, g Quantification of the number of BrdU-positive cells in the mitotic niches at 2 dpIP reveals a clear decrease in both tissues with increasing age, and most obvious between the age of 6 weeks and 12 weeks. b, f Microscopic pictures of BrdU-positive cells in the retina and optic tectum after 21 days of chase demonstrate that BrdU cells are located further away from the neurogenic zones. d, h Measuring the distance from the neurogenic zone to the furthest BrdU-positive cell shows a dramatic decrease in distance in the older age groups. Scale bar = 50 µm. All values are mean ± standard error of mean, n = 3, statistical significance between different conditions is indicated using different letters. dpIP days postintraperitoneal injection, BrdU 5-bromo-2′-deoxyuridine, GCL ganglion cell layer, IPL inner plexiform layer, INL inner nuclear layer, OPL outer plexiform layer, ONL outer nuclear layer, OT optic tectum, PGZ periventricular gray zone.
Functional phenotypes of the senescent killifish visual system
Wondering whether the observed hallmarks might lead to neurodegeneration in the killifish visual system, age-associated changes in retinal morphology were studied on hematoxylin and eosin (H&E)-stained paraffin sections (Fig. 9a). Morphometric analysis of the central retina revealed a significant thinning of the inner retina, i.e., the GCL, inner plexiform layer (IPL), and INL. GCL and INL thinning was most prominent between the age of 12 weeks and 18 weeks (30.6 ± 15.0% and 21.0 ± 8.7%, respectively), while IPL thinning was more seen between the age of 18 weeks and 24 weeks old (10.2 ± 8.4%) (Fig. 9b). Moreover, quantification of the number of neurons in the GCL demonstrated that the number of cells per µm remained rather unchanged between 6 weeks and 12 weeks, while a significant decrease was observed between 12 weeks and 18 weeks (Fig. 9c). As retinal thinning and loss of cells per µm could be due to tissue stretching, we further performed absolute cell counts in the retina. These showed a clear increase in the absolute number of GCL neurons between 6 weeks and 12 weeks, which decreased again between 12 weeks and 18 weeks of age (Fig. 9d). Specifically looking into the number of tyrosine hydroxylase (TH)-positive dopaminergic (displaced) amacrine cells, a neuronal subtype suggested to be affected by aging and in neurodegenerative diseases in mammals as well as in fish29,48,49, in the GCL and INL, revealed a similar increase in cell number between 6 weeks and 12 weeks of age, and decrease between 12 weeks and 18 weeks (Fig. 9e; Supplementary Fig. 2). Altogether, while these data indicate that ocular growth and expansion occur, and thereby confound interpretation of the data, at least part of the observed retinal thinning and cellular loss might reflect true neurodegenerative pathology in the senescent female killifish visual system.
a Representative pictures of hematoxylin and eosin-stained retinal paraffin sections reveal a clear decrease in retinal thickness in 12-week-old fish, and this thinning further continues with increasing age. Scale bar = 100 µm. b Quantification of the thickness of all retinal layers on mid-sagittal paraffin sections shows a gradual thinning, with a significant decline in the GCL, IPL, and the INL. n ≥ 3. c Further quantification of the total number of neurons in the GCL per µm reveals a significant decline in cells per µm between 12 weeks and 18 weeks. n ≥ 3. Counting absolute numbers of neurons in the GCL (d) and of TH-positive dopaminergic (displaced) amacrine cells located in the GCL and INL (e) of the retina demonstrates an age-associated increase in cell number between 6 weeks and 12 weeks, and decrease between 12 weeks and 18 weeks of age. n ≥ 3 for d and n = 10 for e. All values are shown as mean ± standard error of mean, statistical significance between different age groups is shown using different letters. NFL nerve fiber layer, GCL ganglion cell layer, IPL inner plexiform layer, INL inner nuclear layer, OPL outer plexiform layer, ONL outer nuclear layer, RNL rod nuclear layer, CNL cone nuclear layer, PRL photoreceptor segment layer, TH tyrosine hydroxylase.
Finally, to study whether the detected age-related alterations, potentially including neurodegenerative events, in the visual system also result in a decline in functional performance, visual acuity was evaluated in all four killifish age groups using the optokinetic response test. As shown in Fig. 10, visual acuity gradually decreased with age, which is in agreement with findings in zebrafish50. Aging thus clearly affects primary vision in the female killifish.
Evaluating visual acuity of young, middle-aged, old, and very old fish, studied by determining the maximal spatial frequency that causes an optokinetic response, discloses that all age groups retain visual acuity, yet a gradual reduction can be seen in function of age. Data are depicted as mean ± standard error of mean, n ≥ 20, statistical significance between different age groups is indicated using different letters.
Discussion
In 2013, López-Otín et al. identified and categorized several cellular and molecular hallmarks of aging, all contributing to the aging process and together leading to the eventual decay in performance that has been observed in aged individuals7. Also in the brain, many of these hallmarks have been demonstrated and shown to result in a progressive deterioration of functional abilities manifesting as declines in cognitive function, motor coordination, and sensory perception8. A particular functional CNS decrement that many older individuals experience is deterioration and/or loss of vision, which prevents them from performing their daily tasks. While this is partly due to nonneurodegenerative decline, vision loss in elderly is often associated with neuropathology. Indeed, alterations in the human retina during normal aging include some neuronal loss and thinning, especially in the macula, and declined visual performance, which are aggravated in age-associated neuropathologies5,6,51,52,53,54,55. Also in the visual system of the fast-aging killifish, a teleost known to recapitulate many hallmarks of human aging, we detected several age-related alterations that altogether seem to underlie a decrease in vision with age. Note that our data are obtained in female fish, and might conflict with future studies in male animals. Indeed, a large amount of evidence shows a varying prevalence for neurodegenerative diseases between sexes, with for instance Alzheimer’s disease being more prevalent in female individuals and Parkinson’s disease in males56,57. In addition, the pattern of neurogenesis has been demonstrated to be sexually dimorphic in the adult zebrafish forebrain, with gene expression linked with neurogenesis and cell differentiation being altered with age as well differentially expressed in the male and female brain58,59. Moreover, microglial sex differences have been suggested in the homeostatic (aged) mammalian brain60, implying that the observed inflammaging might differ in male killifish as well.
Nevertheless, our findings in female fish are a fine starting point for upcoming research as we show the occurrence of many aging hallmarks in the female killifish visual system. Accumulation of γH2AX, elevated levels of cell cycle regulators, and increased expression of SA-βgal, markers which are highly associated with augmented levels of DNA damage and proliferative arrest, are plausible indicators for the onset of cellular senescence, as already described in mammals7,61 as well as in teleost fish, in which cellular senescence has been shown to manifest in several tissues, including the retina and brain15,29,62,63,64,65,66,67. Our findings revealed an upregulation of these senescence-related markers in the aged killifish retina and tectum, suggesting that cellular senescence very likely manifests in the retinotectal system of old female killifish. Note that our assays preclude the identification of various cell types, and that we thus did not identify which cell types undergo senescence. Although CNS neurons are postmitotic and thus not presumed to become senescent, senescence-related alterations, including augmented SA-βgal signal, have been demonstrated in neurons of aged mice9,68,69,70. As a result, we cannot exclude the appearance of senescent-like neurons in the aged killifish visual system. Despite this, our observations of βgal-positive cells in the optic nerve suggest that at least glial cells show a senescent phenotype, which play an equally important role in CNS integrity and function, and are known to experience cellular senescence upon aging9,61,71.
One specific system that alters upon aging is the innate immune system, in which microglia, the resident immune cells of the CNS, play an essential role. With increasing age, microglia have been shown to undergo morphological changes, increase in number, and (together with neurons and other macroglial cells) alter their secretion pattern toward proinflammatory cytokines (contributing to the SASP), altogether resulting in a process termed inflammaging9,38,39,40,69,72. Similarly in the killifish, microglial number and morphology change upon aging, in the retina as well as in the optic tectum, which is in agreement with findings in the zebrafish retina and killifish brain73,74,75. Moreover, with increasing age, we demonstrate an altered secretory profile, shifting toward raised levels of proinflammatory cytokines (il-8 and tnf) and a downregulation of anti-inflammatory mediators (il-10 and sirt-1). To date, there are no reports in which inflammatory molecules contribute to inflammaging in killifish. A role for IL-8 and TNF in inflammaging has already been described in mammals, where their enhanced expression levels are typically observed in the cerebrospinal fluid and brain of aged animals69,76,77,78,79,80. IL-10, a key anti-inflammatory cytokine that suppresses mediators such as TNF and IL-8, is reported to be typically downregulated in the CNS with increasing age81,82, which is in agreement with our results in the killifish visual system. SIRT-1 is the most conserved member of the silent information regulator family encoding a nicotinamide adenine dinucleotide-dependent class III histone deacetylase, and has been reported to have neuroprotective as well as anti-inflammatory properties in the mammalian CNS. In line with our data, its levels, and thereby also its protective role, have been shown to progressively decline during the course of aging83,84,85,86,87. The link of Sirt-1 with aging, neuroprotection, and inflammation has also been shown in zebrafish88,89. Knock-out of sirt-1 in zebrafish resulted in chronic inflammation, increased reactive oxygen species, apoptosis, and ultimately a reduced lifespan88. Rather unexpectedly, and although no significant differences in expression were observed in the optic tectum, levels of il-6 were downregulated with age in the killifish retina. This contrasts mammalian models, in which IL-6 expression has been shown to increase in the old brain68,69,80,81,90,91. However, IL-6 is known to have both pro- and anti-inflammatory activities, so it is possible that in the killifish brain, Il-6 mainly acts as an anti-inflammatory cytokine. Of note, among the Il-6-type cytokines, leukemia inhibitory factor rather than Il-6 seems a key mediator of the inflammatory response in fish, as lif mRNA is upregulated in the zebrafish retina following optic nerve crush or inflammatory stimulation, while il-6 expression remains unchanged92,93. Whether Il-6 might likewise not be the acting or required Il-6 type cytokine in the killifish CNS needs further investigation. Remarkably, expression of il-1b, recognized as a proinflammatory cytokine, diminished in the killifish visual system with increasing age. While often described to augment in the mammalian brain with advancing age78,80,91,94,95, not all studies show this trend of increasing il-1b expression81,96. Its function and role in the age-associated inflammatory status in killifish awaits future studies. Nevertheless, altogether, our results point toward an altered inflammatory profile in the aged killifish visual system, presented as changes in innate immune cell number, microglial morphology, and dysregulation of their secretory phenotype. In addition, our findings indicate that astrocyte-like cells in the killifish visual system are affected by age and become reactivated, as also reported in the old zebrafish retina and killifish brain16,50. Interestingly, in mice, the release of proinflammatory molecules such as TNF by aged microglia has been shown to activate astrocytes97, suggesting that inflammaging might contribute to the observed age-related reactive gliosis in killifish. These data, together with the occurrence of cellular senescence and inflammaging, indicate the significance of glia-glia and neuron–glia interactions for healthy brain aging and CNS repair in an aged setting.
Teleost fish, and thereby also their CNS, exhibit lifelong growth, which is enabled by the continued presence of numerous mitotic regions in the adult retina and brain. In the fish retina, the ciliary marginal zone allows for the constant addition of concentric rings of new neurons in the peripheral retina, and its NSPCs give rise to all retinal neurons, except for the rod photoreceptors which are generated by Müller glia-derived rod progenitors27. The NSPCs of the tectum are located at the peripheral margins of the tectal hemispheres and are responsible for adding concentric crescents of new neurons in the dorsomedial and ventrolateral edges of the PGZ of each lobe. Findings from our Pcna and Sox2 double immunostainings as well as a BrdU pulse-chase experiment clearly show that the NSPCs in the killifish visual system are affected by age. Indeed, in addition to a drastic decline in the number of proliferating NSPCs in the mitotic zones, our BrdU data reveal that less cells are added in aged fish, which might be indicative for a reduced survival of newborn neurons, but more importantly suggests a decreased neurogenic capacity of NSPCs. These results are in line with previously reported findings in both zebrafish and killifish, in which a decreased activity of NSPCs was proposed to lie at the basis of the decline in neurogenesis with older age16,58,75,98. Interestingly, as also the Sox2-positive area declines with age, our results indicate a decrease in total number of NSPCs present in these neurogenic zones. This implies that the stem cell pool diminishes upon aging, which is in agreement with findings from the subventricular zone of the lateral ventricle in aged mice99. What happens to these lost NSPCs remains an open question today. Whether they die or terminally differentiate when growth rate decreases is still to be determined. Notably, the fact that fish continue to grow throughout adulthood is an important factor to keep in mind when interpreting our results. Indeed, the observed reduction in cellular addition corresponds to the decline in growth rate that is detected at later ages, indicating that, at least in part, growth aspects contribute to our findings regarding stem cell exhaustion in older killifish, or more likely the other way around, i.e., reduced addition of new cells might result in a decreased growth rate (chicken–egg theory).
As already described for zebrafish, we show an age-related decline in inner retinal layer thickness29,50,100,101,102, as well as a decrease in the number of neurons in the GCL per µm. In addition, the absolute numbers of neurons in the GCL, and of dopaminergic (displaced) amacrine cells in the GCL and INL, significantly decrease at later ages. Importantly, the interplay between growth and age-associated alterations in the retina adds complexity to the interpretation of these data. Since fast growth, correlated with high neurogenic ability, is observed until the age of 12 weeks, the increase in absolute number of cells in the inner retina of 12-week-old fish compared to 6-week-old fish is likely due to cellular addition via the ciliary marginal zone of the retina and thus growth of the retina. In addition to growth by cellular addition, growth of the fish retina and optic tectum is also effectuated by tissue stretching, which results in increased distance between cells27,29,103 and might contribute to the observed retinal thinning and decrease in cell number per µm in the GCL. Our results indeed confirm retinal stretching between 6 weeks and 12 weeks of age, as the absolute number of neurons in the GCL increases but the number of neurons per µm remains fairly unchanged. The role of both cellular addition and retinal stretching during retinal growth in fish of different ages has been elaborately described by Van houcke et al., who reported that, at older age, growth is mainly supported by cellular addition and not tissue stretching29. As growth slows down in the killifish after the age of 12 weeks, just like it does after 24 months in zebrafish, we can assume a similar mechanism. A diminished neurogenic potential presumably drives the slower growth rate at later ages, but cannot account for the significant reduction in the absolute number of cells in the inner retina of 18-week- and 24-week-old fish when compared to 12-week-old fish, indicative for a loss of neurons and thus neurodegeneration at older age. Neuronal cell death was investigated via immunohistochemistry for activated-caspase-3 on retinal sections, yet no significant difference in the number of apoptotic cells was detected amongst the different age groups (data not shown). In our opinion, however, this does not exclude cell death, as neurodegeneration manifests gradually and over a long timescale, and this marker only labels cells in a specific phase of the apoptotic process, at one specific moment in time. Altogether, our findings suggest an outspoken correlation of growth and retinal changes before 12 weeks of age, which tends to decrease at later ages, thereby showing the coexistence of a decreased growth rate and neurodegenerative signs. Interestingly, neurodegeneration has also been shown in several brain areas of Nothobranchius species, first by Terzibasi et al., and later by Liu et al., who detected neurofibrillary degeneration in the aged optic tectum, telencephalon, brainstem, and cerebellar cortex using the Fluoro-Jade B dye64,65,104. More recently, an age-dependent deterioration of dopaminergic and noradrenergic neurons, and signs for α-synuclein and amyloid pathology have been reported in the killifish48. Our data now confirmed this dopaminergic cell loss in the aged killifish retina.
Despite the advances that have been made in biogerontology research regarding the molecular and cellular changes that occur during aging, the mechanisms of physiological aging and their interrelation with the development of neurodegenerative disorders definitely require further studies. The African turquoise killifish, which ages within a few months and experiences similar aging processes that take years in other vertebrates, provides a unique model to grasp the underlying cellular and molecular aging processes, and to identify how to tackle them. We introduce the retinotectal system of female fish as an excellent and well accessible in vivo system to understand physiological aging and age-related neuropathies. In addition, the onset of the described age-related changes can be exploited at morphological, molecular, and even functional level, and therefore used as readouts for the efficacy of supportive and/or restorative interventions, hereby positioning this fish as a useful model for drug discovery for healthy aging, brain rejuvenation, and neuroregenerative approaches.
Methods
Fish and husbandry
Killifish (N. furzeri) on a GRZ-AD background (named after Gona Re Zhou National Park in Zimbabwe where they were originally collected; later maintained by Alexander Dorn) were used and kept under standardized conditions, i.e., a 12 h/12 h light/dark cycle and water parameters consisting of a constant water temperature of 28.3 °C, conductivity of 600 µS and pH 7. Adult fish were housed in a ZebTec system (Tecniplast) with a density of four fish (three females; one male to assure female fish can spawn their eggs) per 3.5 L tank and fed twice a day with frozen red bloodworms (Chironomidae; Ocean Nutrition). As male and female fish differ in size and to exclude this size variation within one age group, all experiments were performed using female fish only. We used four adult age groups, i.e., young (6 weeks), middle-aged (12 weeks), old (18 weeks), and very old fish (24 weeks), which were determined based on lifespan experiments performed in our killifish facility (for details see ref. 73). Female fish of the GRZ-AD strain reach sexual maturity around 5 weeks of age. Fish of 6 weeks, in which no mortality was yet observed, were considered young adults. The middle-aged group of 12 weeks was determined by a 90% survival rate. Fish of 18 weeks were denoted old fish as, at this age, we observed an increased mortality and only 70% of the population was still alive. Fish of the oldest population, i.e., very old fish, had an age of 24 weeks, in which only about 50% of the population was still alive. For all experiments (except for the growth rate experiment), uniformly sized fish were used, with body lengths of 2.85 ± 0.03 cm for 6-week-old fish, 3.75 ± 0.03 cm for 12-week-old fish, 4.13 ± 0.04 cm for 18-week-old fish, and 4.35 ± 0.03 cm for 24-week-old fish. All experiments were approved by the institutional Animal Ethics Committee of the KU Leuven and strictly followed the European Communities Council Directive of 20 October 2010 (2010/63/EU).
Growth rate determination in the killifish retinotectal system
For assessment of the growth rate of GRZ-AD killifish, body length, retinal perimeter, retinal surface, tectal perimeter, and tectal surface were determined. Body length was measured as the distance from the snout to the end of the tail fin. Retinal surface was determined on wholemounts, and retinal perimeter and tectal perimeter/surface were assessed, respectively, on central sagittal and coronal sections (see below), all using FIJI. These parameters were evaluated using female fish of all four age groups, i.e., in young adult, middle-aged, old, and very old killifish. Next, growth rate was calculated as the difference in length/perimeter/surface between two consecutive age groups, divided by six to obtain a growth rate value per week. Per age group, at minimum five fish were analyzed.
RNA isolation and RT-qPCR
To assess the transcript levels of several age-associated genes in the killifish visual system, RT-qPCR was performed on both whole retina and optic tectum samples of young adult, middle-aged, old, and very old killifish. Briefly, fish were euthanized using 0.1% Tris-buffered tricaine, after which retinas and optic tecti were quickly dissected on ice. Per fish, the left as well as the right retina/optic tectum were pooled and digested using Tri reagent (Sigma-Aldrich). Total RNA was purified by the NucleoSpin RNA isolation kit (Macherey-Nagel, Germany). Next, oligo dT primers and Superscript III reverse transcriptase (Invitrogen) were used to reverse transcribe RNA to cDNA. All RT-qPCR reactions were carried out using SYBR Green master mix (Bio-Rad) and a CFX96 Touch Real-Time detection system (Bio-Rad). The samples were run in duplicate, with at least four independent samples per age group and an annealing temperature of 60 °C. Selection of reference genes and determination of gene expression values were performed using qBase software (Biogazelle). Primer sequences are listed in Table 1. All values are represented as expression values relative to 6-week-old values, normalized against the geometric mean of our selected reference genes.
BrdU labeling
For labeling of newly generated cells in the neurogenic zones of both the retina and the optic tectum, fish were injected with BrdU (Sigma-Aldrich). Killifish were briefly anesthetized in 0.03% Tris-buffered tricaine and intraperitoneally injected with BrdU solution (8 µg/g body weight). Two injections were performed with a 4-h interval. The animals were sacrificed at 2 or 21 dpIP. To visualize newborn cells, immunohistochemistry for BrdU was performed on cryosections as described below. Prior to the blocking step, DNA breaks were induced by incubating the slides in 2 N HCl at 37 °C, followed by incubation with 0.1 M borate buffer to neutralize the acid. Per experimental condition, the retina/optic tectum of at least five fish were morphologically evaluated and three of them were quantitatively analyzed. On the 2 dpIP sections, BrdU-positive NSPCs within the neurogenic zones, i.e., the ciliary marginal zone of the retina, and the dorsomedial and ventrolateral zones of the tectum, were counted. For the sections of the 21 dpIP condition, the distance from the neurogenic niche to the most distal cell of the BrdU-positive cell cluster was measured. Quantification was performed on six sections per fish, and then averaged per fish.
Immunohistochemistry and histomorphometric analysis
For immunohistochemistry and histomorphometric analysis, killifish were euthanized by submersion in 0.1% Tris-buffered tricaine, and transcardially perfused with phosphate-buffered saline (PBS; 0.01 M; pH 7.4), followed by 4% paraformaldehyde (PFA) in PBS. Next, eyes and brains were dissected and postfixed in PFA for 1 h at room temperature (for immunolabeling) or overnight at 4 °C (for SA-βgal staining), or overnight in Bouin Hollande at 4 °C (for H&E staining). Following appropriate tissue processing, 7 µm paraffin sections or 10 µm cryosections of the retina (sagittal), and 10 µm cryosections of the brain (coronal, at the level of the optic tecti) were made. After stainings, sections were mounted with Mowiol or DePeX, and covered with a glass cover slide.
Retinal morphology was studied on H&E-stained paraffin sections. Histological pictures were taken with a microscope Leica imager at 20× magnification. For morphometric analysis of the retinal thickness, all different layers of the retina were measured on sections through the central retina at 200 µm from the optic nerve head, at the dorsal as well ventral side of each section. In addition, neurons in the GCL were counted over the entire perimeter of the retina. Using FIJI software, at least three mid-sagittal sections (max 240 µm from the central section) per fish were analyzed. All measurements were then averaged per fish. Three to five fish were studied per age group.
Expression of SA-βgal was investigated on cryosections of both eyes and brains, pretreated in PBS pH 6. Next, the slides were incubated overnight in 5 mM potassium ferrocyanide, 5 mM potassium ferricyanide, 2 mM MgCl2, and 1 mg/ml X-Gal (Panreac Applichem) in PBS pH 6 at 37 °C. Using a Zeiss Imager Z1 microscope, pictures at 20× magnification were made. Per age group, at least three fish were quantified by determining the area occupied by SA-βgal signal over an entire retinal section (covering all neuroretinal layers) or tectal section (including both the PGZ and optic tectum) via automated thresholding using FIJI (RenyiEntropy), which was then put relative to the total measured surface area.
Immunolabelings were performed on cryosections. The following primary antibodies were used: rat anti-BrdU (1:400; Abcam), rabbit anti-Ho-1 (1:100; Enzo Life Sciences), rabbit anti-L-plastin (1:200; Genetex), mouse anti-Pcna (1:500; Abcam), rabbit anti-Sox2 (1:1000; Sigma-Aldrich), rabbit anti-TH (1:400; Merck-Millipore), mouse anti-vimentin (1:400; Sigma-Aldrich), and rabbit anti-γH2AX (1:200; Genetex). Detailed information of the primary antibodies used is listed in Table 2. Alexa-conjugated secondary antibodies or a horseradish peroxidase-labeled antibody (Dako) in combination with the Tyramide Signal Amplification technology (TSATM; PerkinElmer) were used for detection. All immunostainings were visualized using an Olympus FV1000 confocal microscope at 20× or 60× magnification. TH-positive cells were counted over the entire retina on at least four mid-sagittal sections, and then averaged per fish. Ten fish were studied per age group. Stainings for Ho-1, L-plastin, Pcna/Sox2, vimentin, and γH2AX were quantified using FIJI by determining the immunopositive area within a predetermined region. Briefly, for the Pcna/Sox2 double labeling, the area of Sox2-positive cells was outlined and measured, followed by manual thresholding and analysis of the Pcna-immunopositive area within this region. For the Ho-1, L-plastin, and vimentin stainings, a fixed area within the central retina (~0.04 mm2, covering all neuroretinal layers, with the GCL excluded for quantification of the Ho-1 and vimentin stainings as Müller glia endfeet would interfere with the analysis) or tectum (0.10 mm2 for the vimentin staining, with the PGZ excluded from the analysis since we were most interested in quantifying expression in the glial fibers, or 0.15 mm2 for the L-plastin staining) was first delineated. Next, the area occupied by L-plastin-positive cells or vimentin-positive signal within this region was defined via automated thresholding (Default, RenyiEntropy and Otsu for Ho-1, L-plastin, and vimentin, respectively), and divided by that region. Finally, for the γH2AX staining, the immunopositive signal was determined within one specific retinal and tectal nuclear layer via manual thresholding, i.e., in a predefined area in the INL (~0.02 mm2) and the PGZ (~0.04 mm2) for the retina and the optic tectum, respectively. Quantification was performed on at minimum three mid-sagittal or central coronal sections per fish and then averaged. Three to five fish were analyzed per age group.
Western blotting
For quantitative analysis of 4-HNE, fish of the four age groups were first euthanized in 0.1% Tris-buffered tricaine. Next, retinas as well as optic tecti (both left and right) were dissected, pooled per fish, and homogenized in lysis buffer containing 10 mM Tris-HCl pH 8, 1% Triton X-100, 150 mM NaCl, 0.1% SDS, 0.5% sodium deoxycholate, 0.2% sodium azide, and protease inhibitors (Roche). Homogenates were loaded at 20 µg onto 4–12% Bis-Tris gels (Bio-Rad), and then transferred onto nitrocellulose membranes (Bio-Rad). Mouse monoclonal anti-4-HNE (1:100; ab48506; Abcam) primary antibody was incubated overnight at 4 °C, followed by 45 min incubation with goat-anti-mouse horseradish-conjugated secondary antibody (1:5000; Bio-Rad). Protein bands were visualized using a luminol-based enhanced chemiluminescent kit (Bio-Rad) and an imaging system (Bio-Rad; ChemiDoc MP imaging system). Next, bands with molecular weights between 40 and 110 kDa were quantified by densitometry (Image Lab 4.1; Bio-Rad). As a loading control and for normalization of protein values, Swift membrane total protein staining (G-Biosciences) was used. Per age group, three fish were analyzed. All data are shown as relative to the young adult fish (6 weeks), which was set as 100%.
Optokinetic response test
Visual acuity was investigated in 6-week-, 12-week-, 18-week-, and 24-week-old killifish using a virtual-reality chamber (OptoMotry; Cerebral Mechanics; Medicine Hat; AB; Canada). Briefly, fish were anesthetized for a short time in 0.03% Tris-buffered tricaine. Next, they were positioned in a custom-made glass chamber in which a continuous water flow was provided, allowing them to reawaken and breathe while being immobilized. The glass chamber was then placed in the middle of a virtual cylinder, displaying vertical sine wave gratings projected onto four computer monitors. Real-time video feedback was provided via a camera placed above the glass chamber. For each fish, eye movements were recorded and the maximal spatial frequency was determined, keeping velocity and contrast fixed at 15°/s and 100%, respectively. Each trial started with a spatial frequency of 0.02 (c/d), which increased via a staircase procedure. Fish were allowed to adapt to the system one day on beforehand. For every age group, visual acuity of at least 20 fish was assessed.
Statistical analysis
For all statistical tests and creation of representative graphs, GraphPad Prism 8 software was used. Normal distribution was tested using the Kolmogorov–Smirnov normality test, and equality of variances was determined via the Brown–Forsythe test, and these assumptions were met in all cases. Significant differences between age groups were evaluated using one- or two-way ANOVA. Data are represented as mean ± standard error of mean and the exact number of biologically independent samples within each group (n) is indicated in the graphs. For all statistical tests, p < 0.05 was considered significantly different.
Reporting summary
Further information on research design is available in the Nature Research Reporting Summary linked to this article.
Data availability
The data sets generated and/or analyzed during the current study are available from the corresponding author upon reasonable request.
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Acknowledgements
The authors would like to thank Prof. Dr. L. Brendonck and Dr. T. Pinceel for providing African turquoise killifish eggs of the GRZ-AD strain; Rony Van Aerschot, Arnold Van Den Eynde, and Lieve Geenen for the daily fish care and environmental control; and Lut Noterdaeme, Stephanie Mentens, and Marijke Christiaens for their technical support. S.V., S.B., J.V.H., and L.D.G. hold a personal Research Foundation Flanders (FWO Vlaanderen, Belgium) fellowship (1S1667N, 1165020N, 1S00318N, and 12I3820N, respectively). Killifish housing and breeding is financially supported by a small equipment grant KA-16-00745 (KU Leuven).
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Conceptualization: S.V., S.B., and L.M.; methodology: S.V., S.B., A.B., and I.E.; writing—original draft preparation: S.V.; writing—review and editing: all authors; visualization: S.V. and S.B.; and supervision: L.M.
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Vanhunsel, S., Bergmans, S., Beckers, A. et al. The killifish visual system as an in vivo model to study brain aging and rejuvenation. npj Aging Mech Dis 7, 22 (2021). https://doi.org/10.1038/s41514-021-00077-4
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DOI: https://doi.org/10.1038/s41514-021-00077-4
