Bidirectional Wnt signaling between endoderm and mesoderm confers tracheal identity in mouse and human cells

Kishimoto, Keishi; Furukawa, Kana T.; Luz-Madrigal, Agustin; Yamaoka, Akira; Matsuoka, Chisa; Habu, Masanobu; Alev, Cantas; Zorn, Aaron M.; Morimoto, Mitsuru

doi:10.1038/s41467-020-17969-w

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Published: 27 August 2020

Bidirectional Wnt signaling between endoderm and mesoderm confers tracheal identity in mouse and human cells

Nature Communications volume 11, Article number: 4159 (2020) Cite this article

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Abstract

The periodic cartilage and smooth muscle structures in mammalian trachea are derived from tracheal mesoderm, and tracheal malformations result in serious respiratory defects in neonates. Here we show that canonical Wnt signaling in mesoderm is critical to confer trachea mesenchymal identity in human and mouse. At the initiation of tracheal development, endoderm begins to express Nkx2.1, and then mesoderm expresses the Tbx4 gene. Loss of β-catenin in fetal mouse mesoderm causes loss of Tbx4⁺ tracheal mesoderm and tracheal cartilage agenesis. The mesenchymal Tbx4 expression relies on endodermal Wnt activation and Wnt ligand secretion but is independent of known Nkx2.1-mediated respiratory development, suggesting that bidirectional Wnt signaling between endoderm and mesoderm promotes trachea development. Activating Wnt, Bmp signaling in mouse embryonic stem cell (ESC)-derived lateral plate mesoderm (LPM) generates tracheal mesoderm containing chondrocytes and smooth muscle cells. For human ESC-derived LPM, SHH activation is required along with WNT to generate proper tracheal mesoderm. Together, these findings may contribute to developing applications for human tracheal tissue repair.

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Introduction

The mammalian respiratory system is crucial for postnatal survival, and defects in the development of the respiratory system cause life-threatening diseases in breathing at birth¹. The trachea is a large tubular air path that delivers external air to the lung. Abnormal development of the tracheal mesenchyme, including cartilage and smooth muscle (SM), is associated with congenital defects in cartilage and SM such as trachea–esophageal fistula (TEF) and tracheal agenesis (TA)^2,3. Thus, understanding trachea development is crucial to better understand TEF/TA and establish a protocol to reconstruct trachea from pluripotent stem cells for human tissue repair.

Trachea/lung organogenesis is coordinated by endodermal-mesodermal interactions during embryogenesis. The primordial tracheal/lung endoderm appears at the ventral side of the anterior foregut at embryonic day 9–9.5 (E9.0–9.5) in mouse (Fig. 1a). Previous studies have demonstrated that development of tracheal/lung endoderm is initiated by graduated expression of mesodermal Wnt2/2b and Bmp4 along the dorsal-ventral axis^4,5,6,7. This mesodermal-to-endodermal Wnt and Bmp signaling drives expression of Nkx2.1, the key transcription factor of tracheal/lung lineage⁸, at the ventral side of the anterior foregut endoderm, which in turn suppresses Sox2 to segregate these Nkx2.1⁺ endodermal cells from the esophageal lineage. The Nkx2.1⁺ endoderm then invaginates into the ventral mesoderm to form the primordial trachea and lung buds. At the same time, the Sox2⁺ endoderm at the dorsal side develops into the esophagus by E10.5 (Fig. 1a)⁹. These studies have demonstrated that mesodermal cells secrete growth factors (e.g., Wnt and Bmp) to induce respiratory endoderm identity^4,5,6.

**Fig. 1: Activation of Wnt signaling in endoderm, but not *Nkx2.1* expression, is activated to promote mesodermal development of the mouse trachea.**

The tracheal mesoderm originates in the ventral fold of lateral plate mesoderm (LPM) surrounding the anterior foregut endoderm. After induction of endodermal Nkx2.1 expression at E9.5, tracheal/lung mesoderm is defined by Tbx4/5 at E10.5, which are markers for tracheal/lung mesoderm and required for proper mesenchymal development (Fig. 1a)¹⁰. In contrast to Tbx5 which is also expressed in LPM and cardiac mesoderm^11,12, Tbx4 expression is restricted to respiratory tissue. At E9.5, Tbx4 is only detected in lung bud mesoderm but not tracheal mesoderm (Supplementary Fig. 1). Tbx4 expression is then detected in tracheal mesoderm from E10.5. Tbx4 and Tbx5 cooperate to steer normal trachea development. Both genes are required for mesodermal development of the trachea, particularly for cartilage and smooth muscle differentiation as well as morphogenesis. The crucial functions of these genes are validated by Tbx4, 5 double mutants exhibiting the phenotypes of tracheal stenosis¹⁰. We previously reported that synchronized polarization of mesodermal cells and temporal initiation of cartilage development regulate tracheal tube morphogenesis by coordinating the length and diameter of the mouse trachea, respectively^13,14. However, the mechanism underlying the initial induction of tracheal mesoderm is still unclear.

Here, we propose that this communication is bidirectional between endoderm and mesoderm. In our model, once tracheal endoderm is specified around E9.5, endodermal cells express Wnt ligands to induce Tbx4 expression in tracheal mesoderm after E10.5. To substantiate the model, we address the following key issues: (1) tracheal endoderm secretes Wnt ligands; (2) tracheal mesoderm responds to endodermal Wnt ligands to specify mesodermal identity through Tbx4 expression; (3) Tbx4 is a direct Wnt target gene.

Results

Endodermal Wnt activity but not Nkx2.1 initiates Tbx4 expression in mouse tracheal mesoderm

To study the initiation of the mesodermal development of the trachea, we validated the involvement of Nkx2.1 in mesodermal Tbx4 expression because endodermal-mesodermal interactions orchestrate organogenesis throughout development in general. Nkx2.1 is an endodermal transcription factor necessary for tracheal and lung development and its genetic ablation results in TEF⁸. We examined Nkx2.1^null mouse embryos and confirmed the TEF phenotype with a single trachea–esophageal (Tr–E) tube (Fig. 1b). Interestingly, Nkx2.1^null embryos retained Tbx4 expression in the ventrolateral mesoderm of a single Tr–E tube, although the segregation was defective (Fig. 1b), indicating that mesodermal induction of the trachea is independent of endodermal Nkx2.1. We compared the phenotype of Nkx2.1^null with that of Shh^Cre, Ctnnb1^flox/flox embryos, which also show anterior foregut endoderm segregation defect and loss of Nkx2.1 expression (Fig. 1c, d)^4,5. In contrast to Nkx2.1^null embryos, Shh^Cre, Ctnnb1^flox/flox embryos did not express Tbx4. To eliminate the possibility that the lack of LPM caused no Tbx4 expression phenotype, we assessed the expression of Foxf1, a pan-LPM marker at this developmental stage (Supplementary Fig. 2a). Foxf1 was still expressed in the mesoderm. This observation suggests that the activation of endodermal Wnt signaling, but not Nkx2.1 expression, is required for following mesodermal Tbx4 expression. Thus, the initial induction of tracheal mesoderm is independent of known Nkx2.1-mediated respiratory endoderm development, but dependent on the activation of Wnt signaling at the ventral anterior foregut endoderm.

Endodermal-to-mesodermal Wnt signaling induces Tbx4 expression in tracheal mesoderm

To further study the spatiotemporal regulation of canonical Wnt signaling during trachea–esophageal segregation at E9.5 to E11.5, we used a reporter line LEF1^EGFP and examined the distribution of EGFP in the canonical Wnt signaling response (Fig. 2a, b)¹⁵. At E9.5, EGFP was detected in the ventral half of the anterior foregut endoderm where trachea endodermal cells appear and express Nkx2.1 (Fig. 2a, b, arrowheads) and then decreased temporally at E10.5. After E10.5, the EGFP reporter was activated in the surrounding mesoderm and its intensity increased at E11.5 (Fig. 2a, b, arrowheads), which was similar to the patterning of Axin2-LacZ, another reporter line for the response of canonical Wnt signaling¹⁶. We further conducted RNAscope in situ hybridization (ISH) against Axin2, an endogenous Wnt target gene, to confirm activation of Wnt signaling in mesoderm. Axin2 was highly expressed in surrounding mesoderm at E10.5 compared to endoderm, similar to the pattern observed in the reporter line (Fig. 2c). Because these Wnt-responsive mesodermal cells expressed Tbx4 (Fig. 2b), we hypothesized that Wnt signaling in the early mesoderm is involved in the initiation of the tracheal mesoderm.

**Fig. 2: Wnt signaling is activated to promote mesodermal development of the mouse trachea.**

To validate the role of mesodermal Wnt signaling, we genetically ablated Ctnnb1, also known as β-catenin, which is a core component of canonical Wnt signaling, from embryonic mesoderm. We employed the Dermo1-Cre line, which targets embryonic mesoderm, including tracheal/lung mesoderm, and generated Dermo1^Cre, Ctnnb1^flox/flox mice^17,18,19,20. In the mutant embryos, Tbx4 expression was absent but Foxf1 was retained in the mesoderm at E10.5 (Fig. 2d and Supplementary Fig. 2b), indicating that mesodermal canonical Wnt signaling is necessary for Tbx4 expression. In contrast, endodermal Nkx2.1 expression and tracheoesophageal segregation were not affected, implying that mesodermal Wnt signaling and Tbx4 is dispensable for endodermal development. Supporting the observation of lung buds in Dermo1^Cre, Ctnnb1^flox/flox embryos^20,21, the mutant lung buds still expressed Tbx4 in mesoderm (Supplementary Fig. 3a). Disruption of Wnt signaling in the mesoderm eliminated Tbx4 expression in the tracheal but still detectable in lung mesoderm, suggesting that Wnt-mediated mesodermal Tbx4 induction is a unique system in trachea development but not lung development.

We further found that the Dermo1^Cre, Ctnnb1^flox/flox mutant exhibits tracheal cartilage agenesis. In the mutants, a periodic cartilage ring structure labeled with Sox9 failed to develop at E16.5, and circumferential SM bundles labeled with smooth muscle actin (SMA) were also malformed (Fig. 2e, f) Therefore, mesodermal Wnt signaling is crucial for trachea mesenchymal development, particularly for tracheal cartilage development.

To determine whether Tbx4 is a direct or indirect target of canonical Wnt signaling in respiratory mesoderm, we analyzed the presence of Tcf/Lef-binding sequences in the Tbx4 lung mesenchyme element (Tbx4⁻LME)^22,23. We identified five putative Tcf/Lef-binding sites in Tbx4-LME using Jaspar 2020 database (Fig. 2g)²⁴. Intersecting these binding sites with public databases, such as UCSC browser and ENCODE, showed that these putative Tcf/Lef-binding sites are highly conserved among different vertebrates except for zebrafish and are localized in a region that contains epigenetic marks of distal regulatory elements H3K27Ac, E14.5 lung; H3K4me1, E15.5 lung; p300, postnatal day (PND) 0 lung and chromatin accessibility at E14.5 lung in which the transcription of Tbx4 gene is active^10,25,26,27.

Next, we sought to identify a source of Wnt ligands that initiate mesodermal Tbx4 expression. Due to the essential role of Wnt2 at early tracheal/lung development⁴, we conducted ISH for Wnt2. Wnt2 is transiently expressed in the ventrolateral mesoderm of the anterior foregut at E9.5, which was obviously reduced by E10.5 when Tbx4 was expressed (Figs. 2b and 3a). Wnt2 is most likely not involved in Tbx4 expression after E10.5. This observation prompted us to hypothesize that an endodermal-to-mesodermal interaction but not mesodermal autonomous induction is required for Tbx4 expression. To test this hypothesis, we generated Shh^Cre, Wls^flox/flox mice, in which endodermal Wnt ligand secretion is inhibited by targeting Wntless (Wls) gene essential for exocytosis of Wnt ligands²⁸. This endoderm-specific deletion of Wls resulted in loss of Tbx4 expression in the mesoderm, but retained Nkx2.1 expression in the endoderm and Wnt2, and Foxf1 in the mesoderm (Fig. 3b, c and Supplementary Fig. 2c)²⁸, making these mice a phenocopy to Dermo1^Cre, Ctnnb1^flox/flox mice (Fig. 2d). Shh^Cre, Wls^flox/flox embryos also formed lung buds and expressed Tbx4 in the distal lung mesoderm (Supplementary Fig. 3b), supporting our idea that Wnt signaling in mesoderm mainly contributes to initiation of mesodermal development of the trachea, but not of the lung. These findings indicate that the endodermal Wnt ligands are sufficient for trachea mesodermal development. From these observations, we conclude that mesodermal Wnt2 activates endodermal canonical Wnt signaling to promote endodermal Wnt ligand expression independent of Nkx2.1. These Wnt ligands then induce endodermal-to-mesodermal canonical Wnt signaling to initiate tracheal mesoderm specific identity (Fig. 3d). These results also suggest that specification in the trachea endodermal lineage is not necessary for the initial induction of the tracheal mesoderm.

**Fig. 3: Endodermal Wnt ligands induce *Tbx4* expression for tracheal mesoderm development of mouse trachea.**

In the developing mouse trachea, several Wnt ligands are expressed in the endoderm between E11.5 to E13.5, such as Wnt3a, 4, 5a, 6, 7b, 11, and 16^21,28. Current single-cell RNA-seq data have shown the presence of several Wnt ligands including Wnt4, 5a, 5b, 6, and 7b in the respiratory endoderm of mouse embryos at E9.5²⁹. We performed ISH against these Wnt ligands at E10.5 to determine the particular ligand inducing Tbx4 expression in trachea development (Fig. 3e, Supplementary Fig. 5). Wnt4 was expressed in esophageal mesoderm and barely detected in tracheal endoderm. Wnt5a, 5b, and 6 were detected in both the endoderm and mesoderm of the trachea. More importantly, Wnt7b was abundantly expressed in tracheal endoderm, suggesting that Wnt7b might be responsible for the ensuing induction of mesodermal Tbx4 expression.

In vitro recapitulation of trachea mesodermal development using mouse and human embryonic stem cells

By recapitulating developmental processes in vitro, trachea/lung endodermal cells and differentiated epithelial populations have been generated from both mouse and human pluripotent stem cells^30,31,32, and can also be used for disease modeling^33,34,35. However, an established protocol for inducing tracheal/lung mesoderm and differentiated mesenchymal tissue from pluripotent cells has not yet been reported because signaling pathways coordinating the mesodermal development are still undefined. To examine whether Wnt signaling is capable of initiating the differentiation of naïve mesodermal cells to Tbx4⁺ trachea mesodermal cells in vitro, we established a protocol for LPM induction from mouse ESCs (mESCs) by refining the published protocol for LPM induction from human pluripotent stem cells³⁶. Because mouse and human ESCs show different states called naïve and primed, which correspond to pre and postimplantation epiblasts, respectively, we converted mESCs into an epiblast ‘primed’ state that led to middle-primitive streak (mid-PS) cells³⁷. These mid-PS cells were then differentiated into LPM cells (Fig. 4a). At day 5, LPM induction was confirmed by immunocytochemistry (ICC) for Foxf1 and Gata4, which are known to be expressed in LPM including splanchnic mesoderm^38,39 (Fig. 4b). ICC showed that 89% of total cells were Foxf1⁺ LPM. Furthermore, qRT-PCR also showed obvious upregulation of LPM marker genes, such as Foxf1, Gata4, Hoxb6, Prrx1, and Bmp4 (Fig. 4c and e)^{40,41,42,43,44}. Given that previous mouse genetic studies have identified Bmp4 as a crucial regulator of trachea development^6,45, we tested whether canonical Wnt and Bmp4 signaling are sufficient to direct the differentiation of LPM into the tracheal mesoderm (Foxf1⁺/Tbx4⁺). mESC-derived LPM cells were cultured with CHIR99021, a GSK3β inhibitor to stabilize β-catenin and activate canonical Wnt signaling, and Bmp4. At day 6, 89% of total cells became double positive for Foxf1 and Tbx4. qRT-PCR further demonstrated elevated expression of tracheal marker genes such as Tbx5, Wnt2, Bmp4 in addition to Tbx4 (Fig. 4d, e). To further confirm the respiratory characteristics of these cells, we took advantage of the 5 Tcf/lef-binding sequences in Tbx4-LME, which we described in Fig. 2g. We established a luciferase reporter assay by reporter plasmids that express luciferase under the control of Tbx4-LME (Fig. 4f). The reporter plasmid was transfected into mESC-derived LPM and luciferase activity was assessed during differentiation. After 24 h (at day 6), the luciferase activity significantly increased in the presence of CHIR99021 (Fig. 4g). Importantly, the mutated reporter, in which all Tcf/Lef-binding sequences were changed to random sequences (Figs. 2g and 4f), did not respond to CHIR99021. The modest increase of luciferase activity might be due to low transfection efficiency. These results determined that the mESC-derived cells were differentiated into proper tracheal mesoderm at day 6.

**Fig. 4: Generation of trachea mesodermal cells and chondrocytes from mouse ESCs in vitro.**

Because tracheal mesenchyme includes cartilage and smooth muscle, we wondered whether our protocol induces mESC to differentiate into these tissues. At day 12, Sox9⁺ aggregated cell masses positive for Alcian blue staining appeared on the dish, indicative of chondrocytes (Fig. 4h–j). Smooth muscle cells (SMA⁺ cells) concurrently appeared to show fibroblastic morphology and filled the space not filled by the Sox9⁺ cells (Fig. 4h, i). Other chondrogenic markers (Aggrecan, Collagen2a1, Sox5/6, Epiphycan) and smooth muscle markers (Tagln, Collagen1a1) were also present in the differentiated cells (Fig. 4k, l and Supplementary Fig. 7a). These data suggest that the mESC-derived tracheal mesoderm is able to develop into tracheal mesenchyme, including chondrocytes and smooth muscle cells.

Finally, we tested the role of Wnt signaling in the human tracheal mesoderm using human ESCs (hESCs). Human LPM induction was performed by following an established protocol³⁶ (Fig. 5a). Subsequently, the cells were directed to tracheal mesoderm by using CHIR99021 and BMP4. For validating hESC-derived LPM, we checked the common LPM markers at day 2 and confirmed that these markers were abundantly expressed in the LPM (Fig. 5b, c and Supplementary Fig. 8k). Immunostaining determined that 95% of the total cells expressed FOXF1 at day 2 (Fig. 5b). Because Tbx4 is also expressed in the limbs and other fetal mouse tissues²³, we sought additional genetic markers for the tracheal mesoderm. We searched the single-cell transcriptomics dataset of the developing splanchnic mesoderm at E9.5 and identified Nkx6.1 as a marker for mesodermal cells surrounding the trachea, lung, and esophagus²⁹. We performed immunostaining and found that Nkx6.1 was expressed in tracheal and esophageal mesenchyme throughout development (Supplementary Fig. 6). Of note, Nkx6.1 was expressed in esophageal and dorsal tracheal mesenchyme but not ventral trachea, which enabled us to define three subtypes of tracheal-esophageal mesenchyme based on the combination of Tbx4 and Nkx6.1 expression (i.e., Tbx4⁺/Nkx6.1⁺; dorsal tracheal mesenchyme, Tbx4⁺/Nkx6.1⁻; ventral tracheal mesenchyme, Tbx4⁻/Nkx6.1⁺; esophageal mesenchyme) (Supplementary Fig. 6). Having characterized the subtypes of tracheoesophageal mesoderm in vivo, the expression of TBX4 and NKX6.1 in the hESC-derived tracheal mesoderm was examined by ICC and qRT-PCR. Although TBX4 was induced in a Wnt activator dose-dependent manner, NKX6.1 expression was not significantly elevated (Supplementary Fig. 8 a, b), suggesting that human trachea mesodermal development requires an additional factor to become more in-vivo-like. Because the ventral LPM is exposed to SHH in addition to Wnt and Bmp4 during tracheoesophageal segregation^46,47, we assessed whether the SHH activator (PMA; purmorphamine) can improve differentiation from hESC-derived LPM cells into the tracheal mesoderm. As expected, both TBX4 and NKX6.1 expression was upregulated by the SHH activator (Supplementary Fig. 8 c, d). After day 5, the differentiating cells also expressed respiratory markers such as TBX4, TBX5, WNT2, BMP4, and NKX6.1 (Fig. 5d–h, Supplementary Fig. 8k). In this culture condition, CHIR99021 enhanced the expression of TBX4 and NKX6.1 genes in a dose-dependent manner (Supplementary Fig. 8 e, f). We also performed qPCR analysis for FOXF1 expression as a pan-LPM marker. FOXF1 expression was decreased by CHIR99021 but still retained after induction (Supplementary Fig. 8g). This result also reflects the feature of in vivo tracheal mesoderm because FOXF1 expression is decreased in the ventral mesoderm of the mouse trachea (Supplementary Fig. 2a–c). These data indicate that HH/BMP was sufficient to induce FOXF1 expression but HH/BMP/WNT was necessary for human respiratory mesoderm induction. We further estimated the efficiency of the induction by immunostaining for TBX4 and FOXF1, and then confirmed that 83% of total cells were TBX4⁺/FOXF1⁺ double positive cells at day 5 (Fig. 5d). At day 10, NKX6.1 expression was clearly elevated, and 30.3% of the total cells became TBX4⁺/NKX6.1⁺ double positive (Fig. 5e–g) while 18.0% were TBX4⁺/NKX6.1⁻ (Fig. 5g). These data suggest that the half of the cells induced with our protocol are trachea mesodermal cells. Further extended culture induced SOX9⁺ aggregates, which were positive for Alcian blue staining in a Wnt activity-dependent manner (Fig. 5h–l). Likewise in mESC-derived cells, ACTA2⁺ smooth muscle-like fibroblastic cells occupied Sox9⁻ region (Fig. 5i). These cells also expressed chondrogenic markers and smooth muscle cell markers (Fig. 5k, l and Supplementary Fig. 7b).

**Fig. 5: Generation of trachea mesodermal cells and chondrocytes from human ESCs in vitro.**

In this culture system, the removal of BMP4 from the growth factor cocktail did not affect differentiation, implying that exogenous BMP4 activation is dispensable (Supplementary Fig. 8h–j). Because of the obvious upregulation of the endogenous BMP4 gene in the hESC-derived LPM by day 2, endogenous BMP4 may be enough to induce tracheal mesoderm and chondrocytes (Supplementary Fig. 8k). Taken together, these data suggest that Wnt signaling plays a unique role in driving differentiation into tracheal mesoderm and chondrocytes from the LPM, which is conserved between mice and human.

Discussion

This study demonstrates that endodermal-to-mesodermal canonical Wnt signaling is the cue that initiates trachea mesodermal development in developing mouse embryos, which is independent of the previously known Nkx2.1-mediated respiratory tissue development. Based on our knowledge of developmental biology, we successfully generated tracheal mesoderm and chondrocytes from mouse and human ESCs. In our protocol, we stimulated ESC-derived LPM with Wnt, Bmp and SHH signaling to mimic spatial information of the ventral anterior foregut. For induction of respiratory endoderm, Wnt, Bmp, and Fgf signaling are required to direct cells in anterior foregut to differentiate into the respiratory lineage^30,31,32. Thus, Wnt, and Bmp signaling are conserved factors that provide spatial information, while Fgf and SHH are required in endoderm and mesoderm induction, respectively, reflecting the unique signaling pathways in each tissue. Mesoderm induction may need fewer exogenous growth factors because the mesodermal cells themselves are sources of spatial information, such as BMP4 in our protocol.

In our culture system, Bmp4 and Wnt activator were sufficient to induce tracheal mesoderm from mouse ES-derived LPM, but HH signalling was also required for the induction of human tracheal mesoderm. To investigate the involvement of HH signalling in mesodermal development, we examined Shh-null mouse embryo (Supplementary Fig. 9). Reflecting the observation in in vitro differentiation, Tbx4 was still expressed in mutant, suggesting the dispensable role of HH signalling in mouse tracheal mesoderm. By contrast, in human, HH signalling is involved in TEF, but the characteristics of mesoderm is not defined yet⁴⁸. In future study, it would be important to decipher the role of HH signalling on human trachea mesodermal development.

In chicken embryo, misexpression of Tbx4 throughout the respiratory-esophageal region ectopically induces Nkx2.1 expression in distal esophageal endoderm underneath manipulated Tbx4-expressing mesoderm⁴⁹, indicating that mesodermal Tbx4 expression defines tracheal identity in chicken. However, in mouse, we determined that Nkx2.1 expression was not affected in Wnt mutant (e.g., Dermo1^Cre; Ctnnb1^flox/flox, and Shh^Cre, Wls^flox/flox) although Tbx4 expression was completely lost (Figs. 2d and 3b), suggesting that mesodermal Tbx4 expression does not trigger tracheal endoderm specification, which is consistent with retaining Nkx2.1 expression in respiratory explants from Tbx4-null mouse embryos¹⁰. These findings indicate that the mechanism inducing tracheal identity is different between mouse and chicken.

Another question is how trachea and lung are differentially specified, despite the strong commonalities between them. Canonical Wnt signalling induces Tbx4 expression in the tracheal mesoderm but not in the lungs. The trachea and lungs share the maker genes, such as endodermal Nkx2.1 and mesodermal Tbx4, but recent mouse genetics have accumulated the evidence that different combination of geneset regulated the trachea and the lungs individually. For instance, FGF10-null mouse embryos failed lung development, but still develop the trachea⁵⁰. In contrast, Bmpr1a, b-null embryos show the tracheal agenesis phenotype while the mutant embryos have the lung buds⁶. It would be interesting to examine expression patterns of receptors for Fgf10, Bmp, and Wnt in the trachea and lung that may distinguish these organs.

Past studies have proposed that Wnt signaling is required for cartilage formation in the late stage of trachea development^19,28. Tracheal chondrocytes originate in Tbx4-expressing cells, and Tbx4/5 knockout mice show severe defects in chondrogenesis^10,22. The early loss of Tbx4 expression in Wnt mutant might contribute, at least a part, the abnormal structures of cartilage in the late stage of development.

In this study, we were unable to perform tissue-specific targeting for trachea endodermal or mesodermal cells because of multiple Cre-expression patterns in Shh-Cre and Dermo1-Cre mouse lines. For example, Shh is also expressed in the notochord and ventral neural tube⁵¹. Future studies with analyses of respiratory tissue-specific Cre lines would strengthen the evidence demonstrating that mutual interaction between respiratory endoderm and mesoderm is required for the induction of trachea development.

Dermo1^Cre, Ctnnb1^flox/flox mutants display a tracheal cartilage agenesis phenotype. Due to the multiple functions of Ctnnb1 in transcriptional regulation and cellular adhesion, however, it is possible that Ctnnb1 knockout affects not only Wnt-mediated transcriptional regulation but also mesenchymal cell-cell adhesion¹⁹. To exclude this possibility, we examined the distribution of Cdh2 as an adhesive molecule in tracheal mesoderm. Cdh2 expressions in the ventral half of tracheal mesoderm were indistinguishable between control and Dermo1^Cre, Ctnnb1^flox/flox embryos (Supplementary Fig. 3). Furthermore, our luciferase assay showed that respiratory mesenchyme specific cis-regulatory region of Tbx4 is stimulated by CHIR99021 through Tcf/Lef-binding elements in the developing tracheal mesoderm in vitro. These findings suggest that Wnt signaling-mediated transcriptional regulation is important for the induction of tracheal mesoderm.

Recently, Han et al. delineated mesodermal development during organ bud specification using single-cell transcriptomics analyses of mouse embryos from E8.5 to E9.5²⁹. Based on the trajectory of cell fates and signal activation, this group also generated organ-specific mesoderm, including respiratory mesoderm, from hESCs, thereby determining that Wnt, BMP4, SHH, and retinoic acid direct differentiation of hESC-derived splanchnic mesoderm to respiratory mesoderm, supporting our current findings. In our protocol, retinoic acid was not included in the media, but alternatively vitamin A, a precursor for retinoic acid, was supplemented. Endogenous metabolite of vitamin A might affect the differentiation into respiratory mesoderm.

These culture methods could be a strong tool to study human organogenesis and the aetiology of TEA and TA, as well as to provide cellular resource for human tracheal tissue repair.

Methods

Mice

All mouse experiments were approved by the Institutional Animal Care and Use Committee of RIKEN Kobe Branch. Mice were handled in accordance with the ethics guidelines of the institute. Mice were housed in 18–23 °C with 40–60% humidity. A 12-h light/12-h dark cycle was used. Nkx2.1^null, Shh^Cre, Dermo1^Cre, Ctnnb1^flox/flox, Wls^flox/flox mice were previously generated^{13,18,52,53,54}.

In all experiments, at least three embryos from more than two littermates were analyzed. All attempts for replicate were successful. Sample size was not estimated by statistical methods. No data were excluded in this study. All control and mutant embryos were analyzed. We did not distinguish the sex of the embryos. No blinding was done in this study.

Immunostaining

Mouse embryos were fixed by 4% Paraformaldehyde/PBS (PFA) at 4 °C overnight. Specimens were dehydrated by ethanol gradient and embedded in paraffin. Paraffin sections (6-μm) were deparaffinized and rehydrated for staining. Detailed procedure and antibodies of each staining were listed in Supplementary Table 1.

In situ hybridization

Mouse embryos were fixed with 4% PFA/PBS at 4 °C overnight, and then tracheas were dissected. Specimens were incubated in sucrose gradient (10, 20, and 30%) and embedded in OCT compound. Frozen sections (12-μm) were subjected to in situ hybridization. For Wnt2, 4, 5a, 7b probe construction, cDNA fragments were amplified by primers listed in Supplementary Table 2. These cDNA fragments were subcloned into pBluscript SK+ at EcoRI and SalI sites. For Wnt5b and six probes, pSPROT1-Wnt5b (MCH085322) and pSPROT1-Wnt6 (MCH000524) were linearized at SalI sites, The NIA/NIH Mouse 15 K and 7.4 K cDNA Clones were provided by the RIKEN BRC^55,56,57. Antisense cRNA transcripts were synthesized with DIG labeling mix (Roche Life Science) and T3 or SP6 RNA polymerase (New England Biolabs Inc.). Slides were permeabilized in 0.1% Triton-X100/PBS for 30 min and blocked in acetylation buffer. After prehybridization, slides were hybridized with 500 ng/ml of DIG-labeled cRNA probes overnight at 65 °C. After washing with SSC, slides were incubated with anti-DIG-AP antibodies (1:1000, Roche Life Science, 11093274910). Sections were colored with BM-purple (Roche Life Science, 11442074001).

For RNAscope experiments, the RNAscope Multiplex Fluorescent v2 assays (Advanced Cell Diagnostics, 323110) were used. The detailed procedure and probes were listed on Supplementary Table 3.

Cell culture

For mesodermal differentiation from mES cells, C57BL/6J-Chr 12 A/J/NaJ AC464/GrsJ mES cells (The Jackson Laboratory) and EB3 cells (AES0139, RIKEN BioResorce Center) were used. C57BL/6J-Chr 12 A/J/NaJ AC464/GrsJ mES cells were kindly provided by Kentaro Iwasawa and Takanori Takebe (Center for Stem Cell & Organoid Medicine (CuSTOM), Perinatal Institute, Division of Gastroenterology, Hepatology and Nutrition, Cincinnati Children’s Hospital, Cincinnati). EB3 was kindly provided by Dr. Hitoshi Niwa (Department of Pluripotent Stem Cell Biology, Institute of Molecular Embryology and Genetics in Kumamoto University)^58,59. Cells were maintained in 2i + leukemia inhibitory factor (LIF) media (1000 units ml⁻¹ LIF, 0.4 μM PD0325901, 3 μM CHIR99021 in N2B27 medium) on ornithine-laminin coated-dishes³⁷. For mesodermal differentiation of mouse ES cells, cells were digested by TrypLE express (Thermo Fisher Scientific, 12604013) and seeded onto Matrigel-coated 12-well plate. EpiLC were induced by EpiLC differentiation medium (1% knockout serum, 20 ng ml⁻¹ Activin A, 12 ng ml⁻¹ FGF2, and 10 μM Y27632 in N2B27 Medium)³⁷ for 2 days. Lateral plate mesoderm was established by Loh’s protocol with some modification³⁶. EpiLC cells were digested by TrypLE express to single cells and seeded onto Matrigel-coated 12-well plate at the density of 6 × 10⁵ cells per well. The cells around middle-primitive streak was induced by LPM D2 medium composed of 2% B27 Supplement Serum free (Thermo Fisher Scientific, 17504044), 1 × GlutaMax (Thermo Fisher Scientific, 35050061), 20 ng ml⁻¹ basic FGF (Peprotech, AF-100-18B), 6 μM CHIR99021 (Sigma–Aldrich, SML1046), 40 ng ml^-1 BMP4 (R&D Systems, 5020-BP-010), 10 ng ml⁻¹ Activin A (Peprotech, PEP-120-14-10), 10 μM Y27632 (Sigma–Aldrich, Y0503) in Advanced DMEM (Thermo Fisher Scientific, 12491015) for 48 h. After that, LPM was induced by LPM D4 medium composed of 2% B27 Supplement Serum free, 1 x GlutaMax, 2 μM XAV939 (Sigma–Aldrich, X3004), 2 μM SB431542 (Merck, 616461), 30 ng ml⁻¹ human recombinant BMP4 in Advanced DMEM for 24 h. At Day 5, respiratory mesenchyme was induced by Day 5 medium composed of 2% B27 Supplement Serum free, 1 x GlutaMax, 1 μM CHIR99021, 10 ng ml⁻¹ BMP4. Medium were freshly renewed everyday.

H1 (NIHhESC-10-0043 and NIHhESC-10-0062), human embryonic stem cell, was provided by Cincinnati children’s hospital medical center Pluripotent Stem Cell Facility. Cells were maintained in mTeSR1 medium (Stem Cell Technologies) on Matrigel-coated plate. For differentiation of H1 cells to mesodermal cells, confluent cells were digested by Accutase to single cells and seeded onto Geltrex-coated 12-well plate at the dilution of 1:20–1:18 in mTeSR1 with 1 uM Thiazovivin (Tocris). Next day, the cells around middle-primitive streak were induced by cocktails of 6 μM CHIR99021 (Sigma–Aldrich, SML1046), 40 ng ml⁻¹ BMP4 (R&D Systems, 5020-BP-010), 30 ng ml⁻¹ Activin A (Cell Guidance Systems), 20 ng ml⁻¹ basic FGF (Thermo Fisher Scientific), and 100 nM PIK90 (EMD Millipore) in Advanced DMEM/F12 including 2% B27 Supplement minus vitamin A, 1% N2 Supplement, 10 uM Hepes, 100UI mL⁻¹ Penicillin/Streptomycin, 2 mM L-glutamine for 24 h. After that, LPM was induced by LPM D2 medium composed of 1 μM Wnt C59 (Cellagen Technologies), 1 μM A83-01 (Tocris), 30 ng ml⁻¹ human recombinant BMP4 in Advanced DMEM/F12 including 2% B27 Supplement minus V. A., 1 x N2 Supplement, 10uM Hepes, 100UI mL⁻¹ Penicillin/Streptomycin, 2 mM L-glutamine for 24 h. To generate respiratory mesenchyme, we combined 3 uM CHIR99021, 2 uM Purmorphamine (Tocris), and 10 ng ml⁻¹ Bmp4 in Advanced DMEM/F12 medium including 2% B27 Supplement Serum free, 1 x N2 Supplement, 10uM Hepes, 100UI mL⁻¹ Penicillin/Streptomycin, 2 mM L-glutamine from Day 2 to Day 10. Medium was freshly renewed everyday.

Immunocytochemistry

At differentiating process, cells were fixed by 4% PFA for 10 min at room temperature. For intracellular staining, cells were permeabilized by 0.2% TritonX-100/PBS for 10 min at room temperature. After blocking the cells with 5% normal donkey serum or 0.3% Triron-X100/1% bovine serum albumin, cells were incubated with primary antibodies overnight at 4 °C. Then, cells were incubated with secondary antibodies for 1 h at room temperature. Detailed procedure and antibodies of each staining were listed in Supplementary Table 4.

Luciferase reporter assay

The fraction of mouse Tbx4 lung mesenchyme specific enhancer (LME) (mm10, chr11:85,893,703-85,894,206, GenScript, ID U3154EL200-3)²² or Tbx4-LME containing putative Tcf/Lef sites mutated (GenScript, ID U3154EL200-6) were synthesized and cloned into pGL4.23 (luc2/minP) vector (promega).

mESC-derived LPM cells were transfected at day 5 in 150 µl of Opti-MEM (Thermo Fisher Scientific, 31985088) with 2 µl of Lipofectamine Stem (Thermo Fisher Scientific, STEM00003) and 1 µg of pGL4.23 (luc2/minP) containing a fraction of mouse Tbx4-LME or Tbx4-LME containing mutated Tcf/Lef sites. Four hours after transfection the tracheal mesenchyme was induced using day 5 medium in presence or absence of Wnt activator (3 μM CHIR99021) and cells were cultured for 24 h and then lysed and assayed using Dual-Luciferase Reporter Assay System (Promega, E1980).

Alcian blue staining

Cells were fixed in 4% PFA/PBS for 10 min at room temperature. After washing with PBS, cells were incubated with 3% acetic acid for 3 min and then stained with 1% alcian blue/3% acetic acid for 20 min.

Quantitative RT-PCR

Total mRNA was isolated by using the Nucleospin kit (TaKaRa, 740955) according to manufacturer’s instruction. cDNA was synthesized by Super^TM Script^TM VILO cDNA synthesis kit (Thermo Fisher Scientific, 11754050). qPCR was performed by PowerUp^TM SYBR^TM Green Master Mix on QuantStudio 3 or 6. Primer sequences were listed on Supplementary Tables 5 and 6. Data are expressed as a fold-change and were normalized with undifferentiated cells expression.

Statistical analyses

Statistical analyses were performed with Excel2013 (Microsoft) or PRISM8 (GraphPad software). For multiple comparison, one-way ANOVA and two-tailed Tukey’s methods were applied. For paired comparison, statistical significance was determined by F-test and Student’s or Welch’s two-tailed t-test.

Reporting summary

Further information on research design is available in the Nature Research Reporting Summary linked to this article.

Data availability

The authors declare that all data supporting the findings of this study are available within the article and its Supplementary Information files or from the corresponding author upon reasonable request. Source data are provided with this paper (Figs. 4b–e, g, i, 5b–e, g, i and Supplementary Figs. 7 and 8). The datasets generated during the current studies are available in the System Science of Biological Dynamics (SSBD) database⁶⁰. Source data are provided with this paper.

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Acknowledgements

We thank Hinako M Takase and Hiroshi Hamada for Wntless conditional flox mice, Masatoshi Takeichi for Ctnnb1 conditional flox mice, Hironobu Fujiwara for LEF1-EGFP mice, and Animal Resource Development Unit. Hiroshi Niwa kindly provided EB3, mouse ES cells. Kentaro Iwasawa and Takanori Takebe kindly provided C57BL/6 mouse ES cell line. We are grateful to Debora Sinner for the help on RNAscope experiment. We thank Scott Rankin for the help on the enhancer analyses of Tbx4 genes. We also thank Shunsuke Mori and Mototsugu Eiraku for Tbx4 antibody. We thank Yuka Noda and David Luedeke for general technical support. We also thank Shigeo Hayashi and Hazuki Hiraga for primary reading. These studies are supported by the funding from Grants-in-Aid for Scientific Research (B) (17H04185) (20H03693) (M.M.), Young Scientists (17K15133 and 19K16156) (K.K.), Promotion of Joint International Research (A) (18KK0423) (K.K.) of the Ministry of Education, Culture, Sports, Science and Technology, Japan, and from The Takeda Science Foundation for the Life Science (M.M.), and The Uehara Memorial Foundation (K.K.). Partially supported by grant NICHD P01HD093363 to A.M.Z.

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Laboratory for Lung Development and Regeneration, Riken Center for Biosystems Dynamics Research (BDR), Kobe, 650-0047, Japan
Keishi Kishimoto, Kana T. Furukawa, Akira Yamaoka, Chisa Matsuoka & Mitsuru Morimoto
RIKEN BDR—CuSTOM Joint Laboratory, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH, 45229, USA
Keishi Kishimoto, Aaron M. Zorn & Mitsuru Morimoto
Center for Stem Cell & Organoid Medicine (CuSTOM), Cincinnati Children’s Hospital Medical Center, Cincinnati, OH, 45229, USA
Keishi Kishimoto, Agustin Luz-Madrigal & Aaron M. Zorn
Division of Developmental Biology, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH, 45229, USA
Keishi Kishimoto, Agustin Luz-Madrigal & Aaron M. Zorn
Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, 606-8507, Japan
Masanobu Habu & Cantas Alev
Institute for the Advanced Study of Human Biology (ASHBi), Kyoto University, Kyoto, 606-8501, Japan
Cantas Alev

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Contributions

K.K. and M.M. designed the project and performed experiments with the aid of A.L.M., A.Y., C.M., and K.T.F. A.M.Z. analysed single-cell transcriptomics for definitive endoderm and splanchnic mesoderm. A.L.M. performed enhancer analyses of Tbx4 gene and supported human ES cell experiments, A.Y. supported mouse experiments. K.K., K.T.F., and C.M. performed mouse ES cell experiment. C.A. and M.H. contributed to mouse and human embryonic-stem-cell-based lateral plate mesoderm induction and differentiation experiments. K.K. and M.M. wrote the manuscript with the contribution of all authors.

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Kishimoto, K., Furukawa, K.T., Luz-Madrigal, A. et al. Bidirectional Wnt signaling between endoderm and mesoderm confers tracheal identity in mouse and human cells. Nat Commun 11, 4159 (2020). https://doi.org/10.1038/s41467-020-17969-w

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Received: 18 September 2019
Accepted: 24 July 2020
Published: 27 August 2020
DOI: https://doi.org/10.1038/s41467-020-17969-w

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