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Proteomic identification of p53-dependent protein phosphorylation

Oncogene volume 27, pages 4854–4859 (2008)Cite this article

Abstract

The p53 tumor suppressor regulates transcription of target genes. We have previously analysed the p53-dependent proteome and identified novel protein targets. Here we have examined p53-dependent phosphorylation using two-dimensional gel electrophoresis and staining with the fluorescent phosphoprotein dye Pro-Q Diamond. We report that p53 induces phosphorylation of a subset of proteins including Nm23, DJ-1, ANXA1 and PrxII. Our identification of p53-dependent phosphorylation of specific target proteins reveals new aspects of the p53-dependent cellular response and suggests that such posttranslational modifications may contribute to p53-mediated tumor suppression.

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Acknowledgements

We thank Dr Bengt Fadeel (Karolinska Institutet, Stockholm, Sweden) for the rabbit polyclonal anti-Annexin 1 antibody and for helpful comments. This work was supported by the Swedish Cancer Society, the Cancer Society of Stockholm, the Swedish Medical Research Council (VR) and Karolinska Institutet.

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Authors and Affiliations

  1. Department of Oncology-Pathology, Cancer Center Karolinska, Karolinska Institutet, Stockholm, Sweden

    R Rahman-Roblick, K G Wiman & U J Roblick

  2. Ludwig Institute for Cancer Research, Uppsala University, Uppsala, Sweden

    U Hellman

  3. Department of Oncology-Pathology, Karolinska Biomic Center, Karolinska Institutet, Stockholm, Sweden

    S Becker, F G Bader & G Auer

  4. Department of Surgery, University of Schleswig-Holstein, Lübeck, Germany

    F G Bader & U J Roblick

Authors
  1. R Rahman-Roblick
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  2. U Hellman
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  3. S Becker
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  4. F G Bader
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  5. G Auer
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  6. K G Wiman
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  7. U J Roblick
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Corresponding author

Correspondence to K G Wiman.

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Supplementary Information accompanies the paper on the Oncogene website (http://www.nature.com/onc).

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Rahman-Roblick, R., Hellman, U., Becker, S. et al. Proteomic identification of p53-dependent protein phosphorylation. Oncogene 27, 4854–4859 (2008). https://doi.org/10.1038/onc.2008.124

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  • DOI: https://doi.org/10.1038/onc.2008.124

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