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Synaptic vesicle retrieval time is a cell-wide rather than individual-synapse property

Nature Neuroscience volume 14, pages 824–826 (2011)Cite this article

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Abstract

Although individual nerve terminals from the same neuron often differ in neurotransmitter release characteristics, the extent to which endocytic retrieval of synaptic vesicle components differs is unknown. We used high-fidelity optical recordings to undertake a large-scale analysis of endocytosis kinetics of individual boutons in hippocampal rat neurons. Our data indicate that endocytosis kinetics do not differ substantially across boutons from the same cell but instead appear to be controlled at a cell-wide level.

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Figure 1: Variation in endocytosis across boutons.
Figure 2: Cell to cell variations in endocytosis.

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Acknowledgements

We would like to thank members of the Ryan laboratory and J. Dittman for useful discussions and R. Kwan for excellent technical assistance. vGlut-pHluorin was kindly provided by S. Voglmaier and R. Edwards (University of California, San Francisco) and the synaptophysin-pHluorin construct was kindly provided by Y. Zhu (Salk Institute). This work was supported by grants from the US National Institutes of Health (T.A.R.) and the David Rockefeller Graduate School of the Rockefeller University.

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Authors and Affiliations

  1. David Rockefeller Graduate Program of Rockefeller University, New York, New York, USA

    Moritz Armbruster

  2. The Department of Biochemistry, Weill Cornell Medical College, New York, New York, USA

    Moritz Armbruster & Timothy A Ryan

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  1. Moritz Armbruster
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  2. Timothy A Ryan
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Contributions

M.A. and T.A.R. designed the study. M.A. performed the experiments. M.A. and T.A.R. analyzed the data. M.A. and T.A.R. wrote the manuscript.

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Correspondence to Timothy A Ryan.

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The authors declare no competing financial interests.

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Armbruster, M., Ryan, T. Synaptic vesicle retrieval time is a cell-wide rather than individual-synapse property. Nat Neurosci 14, 824–826 (2011). https://doi.org/10.1038/nn.2828

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