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A Carbohydrate Biosensor Surface for the Detection of Uropathogenic Bacteria

Bio/Technology volume 12, pages 1376–1378 (1994)Cite this article

Abstract

We have developed a new surface for use in biosensors that is based on a gold plate covered with a specific carbohydrate receptor structure. The carbohydrate, Galα1–4Gal, was bound covalently via a thioalkylcarboxy–spacer, or adsorbed as a neoglycoprotein, to a two–dimensional gold surface. Both types of surfaces showed high specificity in the binding of the uropathogenic bacteria P–fimbriated Escherichia coli compared to the binding of non–infectious bacteria. The signal to noise ratio is sufficiently high to allow specific detection of the bacteria in biosensor applications.

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References

  1. Karlsson, K.-A. 1989. Animal glycosphingolipids as membrane attachment sites for bacteria. Annu. Rev. Biochem. 58: 309–350.

    Article  CAS  Google Scholar 

  2. Feizi, T. 1993. Oligosaccharides that mediate mammalian cell-cell adhesion. Current Opinion in Structural Biology 3: 701–710.

    Article  CAS  Google Scholar 

  3. Nilsson, B., Larsson, A.-L., Ögren, Y., Back, M. and Nilsson, K.G.I. 1988. An amplification technology for improving sensitivity when measuring components in biological samples. J. Immunol. Methods 108: 237–244.

    Article  CAS  Google Scholar 

  4. Blum, L.J. and Coulet, P.R. 1991. Biosensor Principles and Applications. Marcel Dekker, New York.

  5. Svanborg-Edén, C., Freter, R., Hagberg, L., Hull, R., Leffler, H. and Schoolnik, G. 1982. Inhibition of experimental ascending urinary tract infection by an epithelial cell-surface receptor analogue. Nature 298: 560–562.

    Article  Google Scholar 

  6. Raether, H. 1988. Surface Plasmons on Smooth and Rough Surfaces and on Gratings. Springer Verlag, Berlin.

  7. Bain, C.D. and Whitesides, G.M. 1988. Molecular-level control over surface order in self-assembled monolayer films of thiols on gold. Science 240: 62–63.

    Article  CAS  Google Scholar 

  8. Ihs, A. and Liedberg, B. 1992. Chemisorption of L-cysteine and 3-mercaptopro-pionic acid on gold and copper surfaces:an infrared reflection-adsorption study. J. Interface Colloid Sci. 144: 282–292.

    Article  Google Scholar 

  9. Malmqvist, M. 1993. Biospecific interaction analysis using biosensor technology. Nature 361: 186–187.

    Article  CAS  Google Scholar 

  10. Fägerstam, L.G. and O'Shannessy, D.J. 1993. Surface plasmon resonance detection in affinity technologies, p. 229–252. In: Handbook of Affinity Chromatography. Kline, T. (Ed.). Marcel Dekker, New York.

    Google Scholar 

  11. Badimon, L., Badimon, J.J., Turitto, V.T. and Fuster, V. 1987. Thrombosis, studies under flow conditions. Ann. New York Acad. Sci. 516: 527–540.

    CAS  Google Scholar 

  12. Muramatsu, H., Watanabe, Y., Hikuma, M., Ataka, T., Kubo, I., Tamiya, E. and Karube, I. 1989. Piezoelectric crystal biosensor system for detection of Escherichia coli. Anal. Lett. 22: 2155–2164.

    Article  Google Scholar 

  13. Ebersole, R.C., Foss, R.P. and Ward, M.D. 1991. Piezoelectric cell growth sensor. Bio/Technology 9: 450–454.

    CAS  PubMed  Google Scholar 

  14. Mandenius, C.-F., Welin, S., Danielsson, B., Lundström, I. and Mosbach, K. 1984. The interaction of proteins and cells with affinity ligands covalently coupled to silicon surfaces as monitored by ellipsometry. Anal. Biochem. 137: 106–114.

    Article  CAS  Google Scholar 

  15. Bertilsson, L. and Liedberg, B. 1993. Infrared study of thiol monolayer assemblies on gold:preparation, characterization and functionalization of mixed monolayers. Langmuir 9: 141–148.

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. Carl-Fredrik Mandenius

    Present address: Pharmacia AB, S-112 87, Stockholm, Sweden

  2. Kurt G. I. Nilsson: Corresponding author.

Authors and Affiliations

  1. Glycorex AB, Sölveg. 41, S-223 70, Lund, Sweden

    Kurt G. I. Nilsson

  2. Department of Physics and Measurement Technology, Linköping University, S-581 83, Linköping, Sweden

    Carl-Fredrik Mandenius

Authors
  1. Kurt G. I. Nilsson
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  2. Carl-Fredrik Mandenius
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Nilsson, K., Mandenius, CF. A Carbohydrate Biosensor Surface for the Detection of Uropathogenic Bacteria. Nat Biotechnol 12, 1376–1378 (1994). https://doi.org/10.1038/nbt1294-1376

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