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Transformation and Regeneration of a Nitrogen-Fixing Tree, Allocasuarina Verticillata Lam.

Bio/Technology volume 9, pages 461–466 (1991)Cite this article

Abstract

Hairy root formation was induced by inoculating epicotyl, cotyledon and hypocotyl of Allocasuarina verticillata with two strains of Agrobacterium rhizogenes, A4 (agropine type) or 2659 (cucumopine type). Shoot regeneration from hairy roots induced by strain A4, and from control roots excised from untreated seedlings, required a hormone-enriched medium, whereas roots induced by strain 2659 spontaneously regenerated shoots on hormone-free medium. Newly formed shoots were multiplied and rooted to produce transgenic plants that developed unusually extensive, ageotropic root systems. The transformation was demonstrated by the presence of specific opines and by Southern blot analysis.

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Author notes

  1. Michèle Phelep: Corresponding author.

Authors and Affiliations

  1. BSFT/CTFT (CIRAD, ORSTOM), 45 bis Av. de la Belle Gabrielle, 94130, Nogent sur Marne, France

    Michèle Phelep

  2. Institut des Sciences Végétales, CNRS, 91160, Gif sur Yvette, France

    Michèle Phelep, Annik Petit, Lori Martin & Jacques Tempé

  3. Université de Paris VII and BSFT/CTFT (CIRAD, ORSTOM),

    Emile Duhoux

Authors
  1. Michèle Phelep
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  2. Annik Petit
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  3. Lori Martin
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  4. Emile Duhoux
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  5. Jacques Tempé
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Phelep, M., Petit, A., Martin, L. et al. Transformation and Regeneration of a Nitrogen-Fixing Tree, Allocasuarina Verticillata Lam.. Nat Biotechnol 9, 461–466 (1991). https://doi.org/10.1038/nbt0591-461

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