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Electrically induced NGF production by astroglial cells

Nature Biotechnology volume 15, pages 164–166 (1997)Cite this article

Abstract

A controlled culture system has been developed to induce nerve growth factor (NGF) production in astroglial cells that are cultured on an electrode surface. The electrode potential is alternatively modulated at an amplitude of 300 mV and a frequency of 10 Hz. The electric stimulation triggers NGF production and secretion. The mechanism of the electrically induced NGF production is discussed in association with protein kinase C (PKC) activation.

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References

  1. Carman-Krzan, M., Vigé, X. and Wise, B.C. 1991. Regulation by interleukin-1 of nerve growth factor secretion and nerve growth factor mRNA expression in rat primary astroglial cultures. J. Neurochem. 56: 636–643.

    Article  CAS  Google Scholar 

  2. Lu, B., Yokoyama, M., Dreyfus, C.F. and Black, I.B. 1991. NGF gene expression in actively growing brain glia. J. Neurosci. 11: 318–326.

    Article  CAS  Google Scholar 

  3. Yoshida, K. and Gage, F.H. 1991. Fibroblast growth factors stimulate nerve growth factor synthesis and secretion by astrocytes. Brain Res. 538: 118–126.

    Article  CAS  Google Scholar 

  4. Lindholm, D., Hengerer, B., Zafra, F. and Thoenen, H. 1990. Transforming growth factor-61 stimulates expression of nerve growth factor in the rat CMS. Neuroreport 1: 9–12.

    Article  CAS  Google Scholar 

  5. Kojima, J., Shinohara, H., Ikariyama, Y., Aizawa, M., Nagaike, K. and Morioka, S. 1991. Electrically controlled proliferation of human carcinoma cells cultured on the surface of an electrode. J. Biotechnol. 18: 129–140.

    Article  CAS  Google Scholar 

  6. Kojima, J., Shinohara, H., Ikariyama, Y., Aizawa, M., Nagaike, K. and Morioka, S. 1992. Electrically promoted protein production by mammalian cells cultured on the electrode surface. Biotech. Bioeng. 39: 27–32.

    Article  CAS  Google Scholar 

  7. Pshenichkin, S.P. and Wise, B.C. 1995. Okadaic acid increases nerve growth factor secretion, mRNA stability, and gene transcription in primary cultures of cortical astrocytes. J. Biol. Chem. 270: 5994–5999.

    Article  CAS  Google Scholar 

  8. Tanaka, C. and Nishizuka, Y. 1994. The protein kinase C family for neuronal signaling. Annu. Rev. Neurosci. 17: 551–567.

    Article  CAS  Google Scholar 

  9. Furukawa, Y., Furukawa, S., Omae, F., Awatsuji, H. and Hayashi, K. 1993. Alkylcatechols regulate NGF gene expression in astroglial cells via both protein kinase C- and cAMP-independent mechanisms. J. Neurosci. Res. 35: 522–529.

    Article  CAS  Google Scholar 

  10. Jacquier-Sarlin, M.R., Jornot, L. and Polla, B.S. 1995. Differential expression and regulation of hsp70 and hsp90 by phorbol esters and heat shock. J. Biol. Chem. 270: 14094–14099.

    Article  CAS  Google Scholar 

  11. Hallahan, D.E., Gius, D., Kuchibhotla, J., Sukhatme, V., Kufe, D.W. and Weichselbaum, R.R. 1993. Radiation signaling mediated by Jun activation following dissociation from a cell type-specific represser. J. Biol. Chem. 268: 4903–4907.

    CAS  PubMed  Google Scholar 

  12. Yamazaki, T., Komuro, I., Kudoh, S., Zou, Y., Shiojima, I., Mizuno, T., Takano, H., Heroi, Y., Ueki, K., Tobe, K., Kadowaki, T., Nagai, R., and Yazaki, Y. 1995. Mechanical stress activates protein kinase cascade of phosphorylation in neonatal rat cardiac myocytes. J. Clin. Invest. 96: 438–446.

    Article  CAS  Google Scholar 

  13. Gilchrest, B.A., Park, H.-Y., Eller, M.S. and Yaar, M. 1996. Mechanisms of ultraviolet light-induced pigmentation. Photochem. Photobiol. 63: 1–10.

    Article  CAS  Google Scholar 

  14. Bowman, C.L., Ding, J.-R., Sachs, F. and Sokabe, M. 1992. Mechanotransducing ion channels in astrocytes. Brain Res. 584: 272–286.

    Article  CAS  Google Scholar 

  15. Furukawa, S., Furukawa, Y., Satoyoshi, E. and Hayashi, K. 1986. Synthesis and secretion of nerve growth factor by mouse astroglial cells in culture. Biochem. Biophys. Res. Commun. 136: 57–63.

    Article  CAS  Google Scholar 

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Author notes

  1. Masuo Aizawa: Corresponding author (e-mail: maizawa@bio.titech.ac.jp).

Authors and Affiliations

  1. Department of Bioengineering, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama, 226, Japan

    Sumihiro Koyama, Tetsuya Haruyama, Eiry Kobatake & Masuo Aizawa

Authors
  1. Sumihiro Koyama
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  2. Tetsuya Haruyama
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  3. Eiry Kobatake
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  4. Masuo Aizawa
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Koyama, S., Haruyama, T., Kobatake, E. et al. Electrically induced NGF production by astroglial cells. Nat Biotechnol 15, 164–166 (1997). https://doi.org/10.1038/nbt0297-164

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  • DOI: https://doi.org/10.1038/nbt0297-164

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