Abstract
To assess a drug's toxic or carcinogenic effects on neonatal and adult mice and rats, researchers often carry out oral gavage studies. Whether dosed singly or in various combinations, provided as soluble solutions or as colloidal suspensions, the drug must be delivered in accurate and precise doses. For studies that require newborn mice to receive multiple daily doses, delicately handling neonates to increase their chances of surviving is just as critical as the ability to accurately dose small volumes. To help ensure accurate and precise delivery of drug doses ranging from 5 μl for neonatal mice to 400 μl for adults, the authors adapted an automated pipetting system. By slightly modifying standard gavage needles, the authors delivered, on average, 98–99% of targeted dose volumes to neonatal mice.
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Acknowledgements
We thank Mr. Clyde Ulmer (Z-TECH Corporation) for computer technical support; Mr. Richard Rasmussen (The Bionetics Corporation) for technical support; Oak Ridge Institute of Science and Education for financially supporting Drs. Lee, Ali and Lewis; and the analytical group of Division of Chemistry at NCTR. These studies were funded by Interagency Agreement 224-93-0001 between the National Institute of Environmental Health Sciences and the NCTR/Food and Drug Administration. This document has been reviewed in accordance with United States Food and Drug Administration (FDA) policy and approved for publication. Approval does not signify that the contents necessarily reflect the position or opinions of the FDA nor does mention of trade names or commercial products constitute endorsement or recommendation for use. The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the FDA.
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Lewis, S., Lee, F., Ali, A. et al. Modifying a displacement pump for oral gavage dosing of solution and suspension preparations to adult and neonatal mice. Lab Anim 39, 149–154 (2010). https://doi.org/10.1038/laban0510-149
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DOI: https://doi.org/10.1038/laban0510-149
