Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Original Paper
  • Published:

CrkIII: a novel and biologically distinct member of the Crk family of adaptor proteins

Oncogene volume 22, pages 4799–4806 (2003)Cite this article

Abstract

As the role for adaptor proteins constantly proliferates, appreciation of their importance has never been higher. The Crk family of adaptor proteins is no exception. Currently comprising four members, v-Crk, CrkI, CrkII and Crk-like protein, we have introduced a fifth member, CrkIII. Cloned by the CORT technique, CrkIII is identical in sequence to CrkII until the second of its two SH3 domains, which is disrupted partway through and results in a nonfunctional domain and a unique C-terminal sequence. We have demonstrated the existence of native CrkIII at the message level using RT–PCR and RNAse protection assays, and at the protein level in mouse fibroblasts. We show that CrkII overexpression is capable of enhancing insulin-stimulated ERK activity, whereas CrkIII is not, thus partially characterizing a novel member of the Crk family and elucidating important effects mediated by the c-terminal SH3 domain.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2
Figure 3
Figure 5
Figure 4
Figure 6

Similar content being viewed by others

References

  • Akagi T, Shishido T, Murata K and Hanafusa H . (2000). Proc. Natl. Acad. Sci. USA, 97, 7290–7295.

  • Beitner-Johnson D, Blakesley VA, Shen-Orr Z, Jimenez M, Stannard B, Wang LM, Pierce J and LeRoith D . (1996). J. Biol. Chem., 271, 9287–9290.

  • Birge RB, Fajardo JE, Reichman C, Shoelson SE, Songyang Z, Cantley LC and Hanafusa H . (1993). Mol. Cell. Biol., 13, 4648–4656.

  • Feller SM . (2001). Oncogene, 20, 6348–6371.

  • Feng S, Chen JK, Yu H, Simon JA and Schreiber SL . (1994). Science, 266, 1241–1247.

  • Foschi M, Chari S, Dunn MJ and Sorokin A . (1997). EMBO J., 16, 6439–6451.

  • Fukazawa J, Miyake S, Band V and Band H . (1996). J. Biol. Chem., 271, 14554–14559.

  • Fukui Y, Kornbluth S, Jong SM, Wang LH and Hanafusa H . (1989). Oncogene Res., 4, 283–292.

  • Knudsen BS, Feller SM and Hanafusa H . (1994). J. Biol. Chem., 269, 32781–32787.

  • Lowenstein EJ, Daly RJ, Batzer AG, Li W, Margolis B, Lammers R, Ullrich A, Skolnik EY, Bar-Sagi D and Schlessinger J . (1992). Cell, 70, 431–442.

  • Margolis B, Silvennoinen O, Comoglio F, Roonprapunt C, Skolnik E, Ullrich A and Schlessinger J . (1992). Proc. Natl. Acad. Sci. USA, 89, 8894–8898.

  • Matsuda M, Hashimoto Y, Muroya K, Hasegawa H, Kurata T, Tanaka S, Nakamura S and Hattori S . (1994). Mol. Cell. Biol., 14, 5495–5500.

  • Matsuda M, Tanaka S, Nagata S, Kojima A, Kurata T and Shibuya M . (1992). Mol. Cell. Biol., 12, 3482–3489.

  • Mayer BJ, Hamaguchi M and Hanafusa H . (1988). Nature, 332, 272–275.

  • Okada S, Matsuda M, Anafi M, Pawson T and Pessin JE . (1998). EMBO J., 17, 2554–2565.

  • Sakai R, Iwamatsu A, Hirano N, Ogawa S, Tanaka T, Mano H, Yazaki Y and Hirai H . (1994). EMBO J., 13, 3748–3756.

  • Skolnik EY, Batzer A, Li N, Lee CH, Lowenstein E, Mohammadi M, Margolis B and Schlessinger J . (1993). Science, 260, 1953–1955.

  • Skolnik EY, Margolis B, Mohammadi M, Lowenstein E, Fischer R, Drepps A, Ullrich A and Schlessinger J . (1991). Cell, 65, 83–90.

  • Smith JJ, Evans EK, Murakami M, Moyer MB, Moseley MA, Woude GV and Kornbluth S . (2000). J. Cell Biol., 151, 1391–1400.

  • Smith JJ, Richardson DA, Kopf J, Yoshida M, Hollingsworth RE and Kornbluth S . (2002). Mol. Cell Biol., 22, 1412–1423.

  • Sorokin A and Reed E . (1998). Biochem. J., 334 (Pt 3), 595–600.

  • Sorokin A, Reed E, Nnkemere N, Dulin NO and Schlessinger J . (1998). Oncogene, 16, 2425–2434.

  • Sraer JD, Delarue F, Hagege J, Feunteun J, Pinet F, Nguyen G and Rondeau E . (1996). Kidney Int., 49, 267–270.

  • Tanaka S, Hattori S, Kurata T, Nagashima K, Fukui Y, Nakamura S and Matsuda M . (1993). Mol. Cell Biol., 13, 4409–4415.

  • Tanaka S, Morishita T, Hashimoto Y, Hattori S, Nakamura S, Shibuya M, Matuoka K, Takenawa T, Kurata T and Nagashima K . (1994). Proc. Natl. Acad. Sci. USA, 91, 3443–3447.

  • Vries-Smits AM, Burgering BM, Leevers SJ, Marshall CJ and Bos JL . (1992). Nature, 357, 602–604.

  • Xu W, Harrison SC and Eck MJ . (1997). Nature, 385, 595–602.

Download references

Acknowledgements

This work was supported by National Institutes of Health Grants HL22563, DK 41684, a grant from the A and V Meinerz Foundation and by the Cancer Centre of the Medical College of Wisconsin. Dr PP is the recipient of the National Research Service Award HL10165-01. We also thank Dr Benjamin L Margolis (University of Michigan, Ann Arbor, USA), who participated in the early stages of this project and Dr Michael J Dunn (Medical College of Wisconsin, Milwaukee, USA) for his continuous support and advice.

Author information

Authors and Affiliations

  1. Department of Medicine and Cardiovascular Research Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, 53226, WI, USA

    Simon Prosser, Elena Sorokina, Phillip Pratt & Andrey Sorokin

Authors
  1. Simon Prosser
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Elena Sorokina
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Phillip Pratt
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Andrey Sorokin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Andrey Sorokin.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Prosser, S., Sorokina, E., Pratt, P. et al. CrkIII: a novel and biologically distinct member of the Crk family of adaptor proteins. Oncogene 22, 4799–4806 (2003). https://doi.org/10.1038/sj.onc.1206714

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.onc.1206714

Keywords

This article is cited by

Search

Advanced search

Quick links