Abstract
In this study, we investigated the homing and initiation of division of fluorescently labelled adult mouse bone marrow cells after their intravenous injection into lethally irradiated congenic mice. After 2 h, only 3% of the transplanted cells remained in the blood, and ∼35% could be retrieved from the marrow, liver and spleen in approximately equal numbers. Subsequently, the proportion of injected cells found in blood, liver and spleen decreased further, but increased slightly (to ∼17%) in the marrow. Homing of progenitors followed a similar pattern. At 22 h post transplant, almost half of the injected cells in the blood, liver and spleen had completed a first mitosis; although these did not include progenitors with in vitro clonogenic ability. at the same time, >90% of the injected cells recovered from the marrow had not yet divided. Parallel studies with CD44−/− mice showed these to contain a numerically and functionally normal stem cell population whose homing and activation in either CD44+/+ or CD44−/− hosts appeared unaltered. These results indicate homing mechanisms that favor more stable retention of transplanted marrow cells in the marrow of the recipient, more rapid activation of some of those cells that home to other sites, and a lack of change in either of these responses when either the transplanted or the recipient cells do not express CD44. Bone Marrow Transplantation (2000) 26, 559–566.
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Acknowledgements
We would like to thank Jessyca Maltman, Maya Sinclaire and Gayle Thornbury for excellent technical assistance, Jo-Ann Woo for typing the manuscript, Dr Tak Mak (Amgen Institute, Ontario Cancer Institute, Toronto, ON) for kindly providing the original CD44−/− breeding pairs, and Cangene and StemCell for generous gifts of reagents. This work was supported by the National Cancer Institute of Canada (NCIC) with funds from the Terry Fox Run and NIH P01 HL55435. CJ Eaves is a Terry Fox Cancer Research Scientist of the NCIC.
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Oostendorp, R., Ghaffari, S. & Eaves, C. Kinetics of in vivo homing and recruitment into cycle of hematopoietic cells are organ-specific but CD44-independent. Bone Marrow Transplant 26, 559–566 (2000). https://doi.org/10.1038/sj.bmt.1702536
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DOI: https://doi.org/10.1038/sj.bmt.1702536
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