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Antigenic Difference between Informofers and Protein bound to Polyribosomal mRNA from Rat Liver

Nature New Biology volume 240, pages 90–92 (1972)Cite this article

Abstract

NUCLEAR RNA with DNA-like base composition (heterogeneous nuclear RNA, Hn-RNA) is complexed with globular protein particles called informofers1–4. When mRNA is liberated from polysomes by EDTA or incubation with puromycin, it is isolated as a ribonucleoprotein complex (mRNP)5–8. Polyacrylamide gel electrophoresis has been used to study whether the informofer protein and the protein of polysomal mRNP are completely or partly identical9–11. The protein bound to haemoglobin mRNA is different from the informofer protein, but in rat liver and sheep thyroid polysomes a protein was found with characteristics similar to the informofer protein6,12,13. We have used a new immunological procedure to show that the proteins of the two ribonucleoprotein complexes in the rat liver are immunologically different.

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Authors and Affiliations

  1. Norsk Hydro's Institute for Cancer Research, Norwegian Radium Hospital, Montebello, Oslo, 3

    E. M. LUKANIDIN, S. OLSNES & A. PLHL

  2. Institute of Molecular Biology, The Academy of Sciences of the USSR, Moscow

    E. M. LUKANIDIN

Authors
  1. E. M. LUKANIDIN
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  2. S. OLSNES
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  3. A. PLHL
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LUKANIDIN, E., OLSNES, S. & PLHL, A. Antigenic Difference between Informofers and Protein bound to Polyribosomal mRNA from Rat Liver. Nature New Biology 240, 90–92 (1972). https://doi.org/10.1038/newbio240090a0

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