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Selective Cytotoxicity of Anti-Kappa Serum for B Lymphocytes

Nature New Biology volume 237, pages 18–20 (1972)Cite this article

Abstract

MOUSE lymphocytes can be divided into two distinct populations according to the density of immunoglobulin determinants on their surface. Lymphocytes with a high density of immunoglobulin are marrow-derived, nonthymus-processed, B cells, whereas lymphocytes with little or no immunoglobulin are thymus-derived, T cells1–3. Since more than 95% of mouse immunoglobulin light chains are of the kappa type4, treatment of lymphocyte suspensions with an appropriate dilution of rabbit anti-mouse kappa serum and complement should be cytotoxic for only B lymphocytes. This prediction was tested by using lymphocyte populations enriched for either T or B cells or containing the two cell types in a known proportion.

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Authors and Affiliations

  1. Walter and Eliza Hall Institute of Medical Research, PO Royal Melbourne Hospital, Melbourne, 3050

    J. F. A. P. MILLER, J. SPRENT, A. BASTEN & N. L. WARNER

Authors
  1. J. F. A. P. MILLER
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  2. J. SPRENT
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  3. A. BASTEN
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  4. N. L. WARNER
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MILLER, J., SPRENT, J., BASTEN, A. et al. Selective Cytotoxicity of Anti-Kappa Serum for B Lymphocytes. Nature New Biology 237, 18–20 (1972). https://doi.org/10.1038/newbio237018a0

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