Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

A transcriptional partner for MAD proteins in TGF-β signalling

Nature volume 383, pages 691–696 (1996)Cite this article

An Erratum to this article was published on 26 December 1996

Abstract

The transforming-growth-factor-β (TGF-β) superfamily is critical for establishing mesoderm during early embryogenesis in Xenopus. The transcriptional activation of Mix.2, an immediate-early response gene specific to activin-like members of the TGF-β superfamily, is associated with the rapid appearance of a site-specific DMA-binding activity that recognizes a fifty-base-pair regulatory element known as ARE in the Mix.2 promoter. Cloning of the site-specific DNA-binding component of this activity revealed it to be a new winged-helix transcription factor and a direct target for signalling by the TGF-β superfamily. XMAD2, a recently identified TGF-β signal transducer, forms a complex with the transcription factor in an activin-dependent fashion to generate an activated ARE-binding complex. A model is proposed to explain how TGF-β superfamily signals might regulate the expression of specific genes in the early embryo.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Roberts, A. B. & Sporn, M. B. in Peptide Growth Factors and Their Receptors Vol. 1 (eds Sporn, M. B. & Roberts, A. B.) 419–472 (Springer, New York, 1990).

    Book  Google Scholar 

  2. Massague, J. Annu. Rev. Cell Biol. 6, 597–622 (1990).

    Article  CAS  Google Scholar 

  3. Conlon, F. L., Jones, C. & Smith, J. C. Sem. Cell Dev. Biol. 7, 95–101 (1996).

    Article  Google Scholar 

  4. Dawid, I. B. J. Biol. Chem. 269, 6259–6262 (1994).

    CAS  PubMed  Google Scholar 

  5. Grunz, H. Int. J. Dev. Biol. 40, 279–289 (1996).

    CAS  PubMed  Google Scholar 

  6. Green, J. B. A., New, H. V. & Smith, J. C. Cell 71, 731–739 (1992).

    Article  CAS  Google Scholar 

  7. Smith, J. C., Price, B. M. J., Van Nimmen, K. & Huylebroeck, D. Nature 345, 729–731 (1990).

    Article  ADS  CAS  Google Scholar 

  8. Thomsen, G. & Melton, D. Cell 74, 433–441 (1993).

    Article  CAS  Google Scholar 

  9. Henry, G. L., Brivanlou, I. H., Kessler, D. S., Hemmati-Brivanlou, A. & Melton, D. A. Development 122, 1007–1015 (1996).

    CAS  PubMed  Google Scholar 

  10. Jones, C. M., Lyons, K. M., Lapan, P. M., Wright, C. V. E. & Hogan, B. L. M. Development 115, 639–647 (1992).

    CAS  Google Scholar 

  11. Jones, C. M., Dale, L., Hogan, B. L. M., Wright, C. V. E. & Smith, J. C. Development 122, 1545–1554 (1996).

    CAS  PubMed  Google Scholar 

  12. Koster, M. et al. Mech. Dev. 33, 191–200 (1991).

    Article  CAS  Google Scholar 

  13. Dale, L., Howes, G., Price, B. M. J. & Smith, J. C. Development 115, 573–585 (1992).

    CAS  Google Scholar 

  14. Wilson, P. A. & Hemmati-Brivanlou, A. Nature 376, 331–333 (1995).

    Article  ADS  CAS  Google Scholar 

  15. Jones, C. M., Kuehn, M. R., Hogan, B. L. M., Smith, J. C. & Wright, C. V. E. Development 121, 3651–3662 (1995).

    CAS  PubMed  Google Scholar 

  16. Smith, W. C., McKendry, R., Ribisi, S. & Harland, R. Cell 82, 37–46 (1995).

    Article  CAS  Google Scholar 

  17. Hemmati-Brivanlou, A. & Melton, D. A. Nature 359, 609–614 (1992).

    Article  ADS  CAS  Google Scholar 

  18. Schulte-Merker, S., Smith, J. C. & Dale, L. EMBO J. 13, 3533–3541 (1994).

    Article  CAS  Google Scholar 

  19. Rosa, F. M. Cell 57, 965–974 (1989).

    Article  CAS  Google Scholar 

  20. Huang, H. C., Murtaugh, L. C., Vize, P. D. & Whitman, M. EMBO J. 14, 5965–5973 (1995).

    Article  CAS  Google Scholar 

  21. Mead, P. E., Brivanlou, I. H., Kelley, C. M. & Zon, L. I. Nature 382, 357–360 (1996).

    Article  ADS  CAS  Google Scholar 

  22. Vize, P. Dev. Biol. 177, 226–231 (1996).

    Article  CAS  Google Scholar 

  23. Graff, J. M., Bansal, A. & Melton, D. A. Cell 85, 479–487 (1996).

    Article  CAS  Google Scholar 

  24. Kaufmann, E. & Knochel, W. Mech. Dev. 57, 3–20 (1996).

    Article  CAS  Google Scholar 

  25. Qian, X. & Costa, R. H. Nucleic Acids Res. 23, 1184–1191 (1995).

    Article  CAS  Google Scholar 

  26. Miura, N., Wanaka, A., Tohyama, M. & Tanaka, K. FEBS Lett. 326, 171–176 (1993).

    Article  CAS  Google Scholar 

  27. Evan, G. I., Lewis, G. K., Ramsay, G. & Bishop, J. M. Mol. Cell. Biol. 5, 3610–3616 (1985).

    Article  CAS  Google Scholar 

  28. Hoodless, P. A. et al. Cell 85, 489–500 (1996).

    Article  CAS  Google Scholar 

  29. Baker, J. C. & Harland, R. M. Genes Dev. 10, 1880–1889 (1996).

    Article  CAS  Google Scholar 

  30. Liu, F. et al. Nature 381, 620–623 (1996).

    Article  ADS  CAS  Google Scholar 

  31. Lechleider, R. J., Caestecker, M. P. D., Dehejia, A., Polymeropoulos, M. H. & Roberts, A. B. J. Biol. Chem. 271, 17617–17620 (1996).

    Article  CAS  Google Scholar 

  32. Massague, J. Cell 85, 947–950 (1996).

    Article  CAS  Google Scholar 

  33. Ten Dijke, P. Prog. Growth Factor Res. 5, 55–72 (1994).

    Article  CAS  Google Scholar 

  34. Massagué, J. Cell 69, 1067–1070 (1992).

    Article  Google Scholar 

  35. Lin, H. Y. & Lodish, H. F. Trends Cell Biol. 3, 14–19 (1993).

    Article  CAS  Google Scholar 

  36. Wang, T. W. et al. Science 271, 1120–1122 (1996).

    Article  ADS  CAS  Google Scholar 

  37. Wang, T., Donahoe, P. K. & Zervos, A. S. Science 265, 674–676 (1994).

    Article  ADS  CAS  Google Scholar 

  38. Yamaguchi, K. et al. Science 270, 2008–2011 (1995).

    Article  ADS  CAS  Google Scholar 

  39. Shibuya, H. et al. Science 272, 1179–1182 (1996).

    Article  ADS  CAS  Google Scholar 

  40. Sekelsky, J. J., Newfeld, S. J., Raftery, L. A., Chartoff, E. H. & Gelbart, W. M. Genetics 139, 1347–1358 (1995).

    CAS  PubMed  PubMed Central  Google Scholar 

  41. Arora, K. et al. Cell 81, 781–790 (1995).

    Article  CAS  Google Scholar 

  42. Grieder, N. C., Nellen, D., Burke, R., Basler, K. & Affolter, M. Cell 81, 791–800 (1995).

    Article  CAS  Google Scholar 

  43. Thomsen, G. H. Development 122, 2359–2366 (1996).

    CAS  PubMed  Google Scholar 

  44. Newfeld, S., Chartoff, E., Graff, J., Melton, D. & Gelbart, W. Development 122, 2099–2108 (1996).

    CAS  PubMed  Google Scholar 

  45. Eppert, K. et al. Cell 86, 543–552 (1996).

    Article  CAS  Google Scholar 

  46. Weigel, D. & Jackle, H. Cell 63, 455–456 (1990).

    Article  CAS  Google Scholar 

  47. Watabe, T. et al. Genes Dev. 9, 3038–3050 (1995).

    Article  CAS  Google Scholar 

  48. Li, J. J. & Herskowitz . Science 262, 1870–1873 (1993).

    Article  ADS  CAS  Google Scholar 

  49. Wang, M. M. & Reed, R. R. Nature 364, 121–126 (1993).

    Article  ADS  CAS  Google Scholar 

  50. Bolce, M. E., Hemmati-Brivanlou, A., Kushner, P. D. & Harland, R. M. Development 115, 681–688 (1992).

    CAS  PubMed  Google Scholar 

  51. Whitman, M. & Melton, D. A. Science 244, 803–806 (1989).

    Article  ADS  CAS  Google Scholar 

  52. Nieuwkoop, P. D. & Faber, J. Normal Table of Xenopus laevis (Daudin) (North Holland, Amsterdam, 1967).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts, 02115, USA

    Xin Chen, Melissa J. Rubock & Malcolm Whitman

Authors
  1. Xin Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Melissa J. Rubock
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Malcolm Whitman
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chen, X., Rubock, M. & Whitman, M. A transcriptional partner for MAD proteins in TGF-β signalling. Nature 383, 691–696 (1996). https://doi.org/10.1038/383691a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/383691a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing