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Flavodoxin as an in situ marker for iron stress in phytoplankton

Abstract

A fundamental issue in marine science is the identification of the factors controlling biological uptake of CO2, in high-nitrate, low-chlorophyll regions1–10. A recent in situ iron fertilization experiment demonstrated that iron limitation is responsible for low phytoplankton stocks in the equatorial Pacific4. Here we show that flavodoxin, a biochemical marker of iron limitation, can be used to map the degree of iron stress in natural populations. Flavodoxin assays along a 900-km east-west transect in the northeastern subarctic Pacific revealed a pronounced increase in iron stress in the region west of the 135° W meridian. Addition of dissolved iron alleviated this stress. Immunostaining of single cells from the most western station showed that flavodoxin is present specifically within the chloroplasts of diatoms. Our approach provides a rapid means of defining the extent of iron stress in the ocean5 and supports the hypothesis that diatoms are iron stressed in the northeast Pacific6,10.

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Roche, J., Boyd, P., McKay, R. et al. Flavodoxin as an in situ marker for iron stress in phytoplankton. Nature 382, 802–805 (1996). https://doi.org/10.1038/382802a0

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