Abstract
c-mos protein, encoded by a proto-oncogene, is essential for the meiotic maturation of frog oocytes. Polyadenylation of c-mos messenger RNA is shown here to be a pivotal regulatory step in meiotic maturation. Maturation is prevented by selective amputation of polyadenylation signals from c-mos mRNA. Injection of a prosthetic RNA, which restores c-mos polyadenylation signals by base pairing to the amputated mRNA, rescues maturation and can stimulate translation in trans. Prosthetic RNAs may provide a general strategy by which to alter patterns of mRNA expression in vivo.
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Sheets, M., Wu, M. & Wickens, M. Polyadenylation of c-mos mRNA as a control point in Xenopus meiotic maturation. Nature 374, 511–516 (1995). https://doi.org/10.1038/374511a0
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DOI: https://doi.org/10.1038/374511a0
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