Abstract
Ras (p21ras) interacts directly with the catalytic subunit of phosphatidylinositol-3-OH kinase in a GTP-dependent manner through the Ras effector site. In vivo, dominant negative Ras mutant N17 inhibits growth factor induced production of 3′ hosphorylated phosphoinositides in PC12 cells, and transfection of Ras, but not Raf, into COS cells results in a large elevation in the level of these lipids. Therefore Ras can probably regulate phosphatidylinositol-3-OH kinase, providing a point of divergence in signalling pathways downstream of Ras.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
References
Lowy, D. R. & Willumsen, B. M. A. Rev. Biochem. 62, 851–891 (1993).
Trahey, M. & McCormick, F. Science 238, 542–545 (1987).
Downward, J. Curr. Biol. 1, 353–355 (1991).
Duchesne, M. et al. Science 259, 525–528 (1993).
Medema, R. H. et al. Molec cell. Biol. 12, 3425–3430 (1992).
Schweighoffer, F. et al. Science 256, 825–827 (1992).
Yatani, A. et al. Cell 61, 769–776 (1990).
Marshall, C. J. Curr. Opin. Genet. Dev. 4, 82–89 (1994).
Zhang, X-F. et al. Nature 364, 308–313 (1993).
Vojtek, A. B., Hollenberg, S. M. & Cooper, J. A. Cell 74, 205–214 (1993).
Van Aelst, L., Barr, M., Marcus, S., Polverino, A. & Wigler, M. Proc. natn. Acad. Sci. U.S.A. 90, 6213–6217 (1993).
Warne, P. H., Vicinia, P. R. & Downward, J. Nature 364, 352–355 (1993).
Moodie, S. A., Willumsen, B. M., Weber, M. J. & Wolfman, A. Science 260, 1658–1661 (1993).
Vogel, L. B. & Fujita, D. J. Molec. cell. Biol. 13, 7408–7417 (1993).
Prasad, K. V. S. et al. Molec. cell. Biol. 13, 7708–7717 (1993).
Feramisco, J. R., Gross, M., Kamata, T., Rosenberg, M. & Sweet, R. W. Cell 38, 109–117 (1984).
Schaber, M. D. et al. Prot. Struct. Funct. Genet. 6, 306–315 (1989).
Furth, M. E., Davis, L. J., Fleurdelys, B. & Scolnick, E. M. J. Virol. 43, 294–304 (1982).
Howe, L. R. et al. Cell 71, 335–342 (1992).
Kyriakis, J. M. et al. Nature 358, 417–421 (1992).
Stephens, L. R., Jackson, T. R. & Hawkins, P. T. Biochim. biophys. Acta 1179, 27–75 (1993).
Tuveson, D. A., Carter, R. H., Soltoff, S. P. & Fearon, D. T. Science 260, 986–989 (1993).
Pages, F. et al. Nature 369, 327–329 (1994).
Carpenter, C. L. et al. J. biol. Chem. 268, 9478–9483 (1993).
Backer, J. M. et al. EMBO J. 11, 3469–3479 (1992).
Myers, M. G. et al. Proc. natn. Acad. Sci. U.S.A. 89, 10350–10354 (1992).
Fantl, W. J. et al. Cell 69, 413–423 (1992).
Satoh, T. et al. Molec. cell. Biol. 13, 3706–3713 (1993).
Nishimura, R. et al. Molec. cell. Biol. 13, 6889–6896 (1993).
Page, M. J. et al. J. biol. Chem. 264, 19147–19154 (1989).
Gout, I. et al. Biochem. J. 288, 395–405 (1992).
Reif, K., Gout, I., Waterfield, M. D. & Cantrell, D. A. J. biol. Chem. 268, 10780–10788 (1993).
Harlow, E. & Lane, D. Antibodies: a Laboratory Manual (Cold Spring Harbor Laboratory Press, New York, 1988).
Chung, J., Kuo, C. J., Crabtree, G. R. & Blenis, J. Cell 69, 1227–1236 (1992).
Sjölander, A., Yamamoto, K., Huber, B. E. & Lapetina, E. C. Proc. natn. Acad. Sci. U.S.A. 88, 7908–7912 (1991).
Sjölander, A. & Lapetina, E. C. Biochem. biophys. Res. Commun. 189, 1503–1508 (1992).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rodriguez-Viciana, P., Warne, P., Dhand, R. et al. Phosphatidylinositol-3-OH kinase direct target of Ras. Nature 370, 527–532 (1994). https://doi.org/10.1038/370527a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/370527a0
This article is cited by
-
CD6-mediated inhibition of T cell activation via modulation of Ras
Cell Communication and Signaling (2022)
-
Spatiotemporal dynamics of membrane surface charge regulates cell polarity and migration
Nature Cell Biology (2022)
-
Targeting KRASp.G12C Mutation in Advanced Non-Small Cell Lung Cancer: a New Era Has Begun
Current Treatment Options in Oncology (2022)
-
Hsa_circ_0008344 Promotes Glioma Tumor Progression and Angiogenesis Presumably by Regulating miR-638/SZRD1 Pathway
Neurotoxicity Research (2022)
-
Isoprenylcysteine carboxyl methyltransferase is critical for glioblastoma growth and survival by activating Ras/Raf/Mek/Erk
Cancer Chemotherapy and Pharmacology (2022)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.