Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Putative receptor for inositol 1,4,5-trisphosphate similar to ryanodine receptor

Nature volume 342, pages 192–195 (1989)Cite this article

Abstract

INOSITOL 1,4,5-trisphosphate (Ins(l,4,5)P3) serves as an intracellular second messenger for several neurotransmitters, hormones and growth factors by initiating calcium release from intracellular stores1,2. A cerebellar Ins(l,4,5)P3 receptor has been characterized biochemically3,4 and shown by immunocytochemistry to be present in intracellular membranes in Purkinje cells5. We show that a previously described Purkinje-cell messenger RNA6 encodes a protein of relative molecular mass 260,000 (260 K) with the same properties as the cerebellar Ins(l,4,5)P3 receptor. Its sequence is partially homologous to the skeletal muscle ryanodine receptor7. By immunocytochemistry and electron microscopy the protein is shown to be present in all parts of the endoplasmic reticulum, including those that extend into axon terminals and dendritic spines. Our results indicate that gated calcium release from intracellular stores in muscle and Purkinje cells uses similar calcium-channel proteins localized in analogous intracellular compartments. This implies that the intracellular calcium stores in the endoplasmic reticulum of neurons extend into presynaptic terminals and dendritic spines where they may play a direct role in regulating the efficacy of neurotransmission.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Berridge, M. J. A. A. Rev. Biochem. 56, 159–193 (1987).

    Article  CAS  Google Scholar 

  2. Streb, H., Irvine, R. F., Berridge, M. J. & Schulz, I. Nature 306, 67–69 (1983).

    Article  ADS  CAS  Google Scholar 

  3. Suppattapone, S., Worley, P. F., Baraban, J. M. & Snyder, S. H. J. biol. Chem. 263, 1530–1534 (1988).

    Google Scholar 

  4. Suppattapone, S. et al. Proc. natn. Acad. Sci. U.S.A. 85, 8747–8750 (1988).

    Article  ADS  Google Scholar 

  5. Ross, C. A. et al. Nature 339, 468–470 (1989).

    Article  ADS  CAS  Google Scholar 

  6. Nordquist, D. T., Kozak & Orr, H. T. J. Neurosci. 8, 4780–4789 (1988).

    Article  CAS  Google Scholar 

  7. Takeshima, H. et al. Nature 339, 439–445 (1989).

    Article  ADS  CAS  Google Scholar 

  8. De Camilli, P., Miller, P. E., Levitt, P., Walter, U. & Greengard, P. Neuroscience 11, 761–817 (1984).

    Article  CAS  Google Scholar 

  9. Wouterland, F. G. & Mugnaini, E. Z. J. comp. Neurol. 227, 136–157 (1984).

    Article  Google Scholar 

  10. Mugnaini, E., Berrebi, A. S., Dahl, A. L. & Morgan, J. I. Archiv. Ital. de Biol. 126, 41–67 (1987).

    CAS  Google Scholar 

  11. Mugnaini, E. & Morgan, J. I. Proc. natn. Acad. Sci. U.S.A. 84, 8692–8696 (1987).

    Article  ADS  CAS  Google Scholar 

  12. De Camilli, P., Harris, S. M., Huttner, W. B. & Greengard, P. J. Cell Biol. 96, 1355–1373 (1983).

    Article  CAS  Google Scholar 

  13. Navone, F. et al. J. Cell Biol. 103, 2511–2527 (1986).

    Article  CAS  Google Scholar 

  14. Broadwell, R. D. & Cataldo, A. M. J. comp. Neurol. 230, 231–248 (1984).

    Article  CAS  Google Scholar 

  15. Palay, S. L. & Chan-Palay, V. Cerebellar Cortex, Cytology and Organization (Springer, New York, 1974).

    Book  Google Scholar 

  16. Henkart, M., Landis, D. M. D. & Reese, T. S. J. Cell Biol. 70, 338–347 (1976).

    Article  CAS  Google Scholar 

  17. Rosenbluth, J. J. Cell Biol. 13, 405–421 (1962).

    Article  CAS  Google Scholar 

  18. Gray, E. G. J. Anat. 93, 420–433 (1959).

    CAS  PubMed  PubMed Central  Google Scholar 

  19. Mikoshiba, K., Huchet, M. & Changeux, J. P. Devl Neurosci. 2, 254–275 (1979).

    Article  CAS  Google Scholar 

  20. Walaas, I., Nairn, N. C. & Greengard, P. J. Neurosci. 6, 954–961 (1986).

    Article  CAS  Google Scholar 

  21. Lai, F. A., Erickson, H. P., Rousseau, E., Liu, Q.-Y. & Meissner, G. Nature 331, 315–319 (1988).

    Article  ADS  CAS  Google Scholar 

  22. Südhof, T. C. et al. Science 245, 1474–1480 (1989).

    Article  ADS  Google Scholar 

  23. Johnston, P. A., Jahn, R. & Südhof, T. C. J. biol. Chem. 264, 1268–1273 (1989).

    CAS  PubMed  Google Scholar 

  24. Bon, S., Rieger, F. & Massoulie, J. Eur. J. Biochem. 35, 372–379 (1973).

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. Koji Takei and Pietro De Camilli: Department of Cell Biology, Yale University, New Haven, Connecticut 06510, USA

Authors and Affiliations

  1. Howard Hughes Medical Institute, and Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas, 75235, USA

    Gregory A. Mignery, Thomas C. Südhof, Koji Takei & Pietro De Camilli

Authors
  1. Gregory A. Mignery
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Thomas C. Südhof
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Koji Takei
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Pietro De Camilli
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mignery, G., Südhof, T., Takei, K. et al. Putative receptor for inositol 1,4,5-trisphosphate similar to ryanodine receptor. Nature 342, 192–195 (1989). https://doi.org/10.1038/342192a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/342192a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing