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Structure of the guanine-nucleotide-binding domain of the Ha-ras oncogene product p21 in the triphosphate conformation

Nature volume 341, pages 209–214 (1989)Cite this article

Abstract

The crystal structure of the guanine-nucleotide-binding domain of p21 (amino acids 1–166) complexed to the guanosine triphosphate analogue guanosine-5′-(β, γ-imido)triphosphate (GppNp) has been determined at a resolution of 2.6 Å. The topological order of secondary structure elements is the same as that of the guanine-nucleotide-binding domain of bacterial elongation factor EF-Tu. Many interactions between nucleotide and protein have been identified. The effects of point mutations and the conservation of amino-acid sequence in the guanine-nucleotide-binding proteins are discussed.

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Authors and Affiliations

  1. Max-Planck-lnstitut für medizinische Forschung, Abteilung Biophysik, Jahnstrasse 29, 6900, Heidelberg, FRG

    Emil F. Pai, Wolfgang Kabsch, Ute Krengel, Kenneth C. Holmes, Jacob John & Alfred Wittinghofer

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  1. Emil F. Pai
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  2. Wolfgang Kabsch
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  3. Ute Krengel
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  4. Kenneth C. Holmes
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  5. Jacob John
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  6. Alfred Wittinghofer
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Pai, E., Kabsch, W., Krengel, U. et al. Structure of the guanine-nucleotide-binding domain of the Ha-ras oncogene product p21 in the triphosphate conformation. Nature 341, 209–214 (1989). https://doi.org/10.1038/341209a0

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