Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

A novel potent vasoconstrictor peptide produced by vascular endothelial cells

Abstract

An endothelium-derived 21-residue vasoconstrictor peptide, endothelin, has been isolated, and shown to be one of the most potent vasoconstrictors known. Cloning and sequencing of preproendothelin complementary DNA shows that mature endothelin is generated through an unusual proteolytic processing, and regional homologies to a group of neurotoxins suggest that endothelin is an endogenous modulator of voltage-dependent ion channels. Expression of the endothelin gene is regulated by several vasoactive agents, indicating the existence of a novel cardiovascular control system.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

References

  1. Furchgott, R. F. & Zawadzki, J. V. Nature 288, 373–376 (1980).

    Article  ADS  CAS  Google Scholar 

  2. Furchgott, R. F. Circ. Res. 53, 557–573 (1983).

    Article  CAS  PubMed  Google Scholar 

  3. Furchgott, R. F. A. Rev. Pharmac. Toxicol. 24, 175–197 (1984).

    Article  CAS  Google Scholar 

  4. Palmer, R. M. J., Ferrige, A. G. & Moncada, S. Nature 327, 524–526 (1987).

    Article  ADS  CAS  PubMed  Google Scholar 

  5. DeMey, J. G. & Vanhoutte, P. M. Circ. Res. 51, 439–447 (1982).

    Article  CAS  Google Scholar 

  6. Rubanyi, G. M. & Vanhoutte, P. M. J. Physiol, Lond. 364, 45–56 (1985).

    Article  CAS  PubMed  Google Scholar 

  7. Holden, W. E. & McCall, E. Expl Lung Res. 7, 101–112 (1984).

    Article  CAS  Google Scholar 

  8. Harder, D. R. Circ. Res. 60, 102–107 (1987).

    Article  ADS  CAS  PubMed  Google Scholar 

  9. Katusic, Z. S., Shepherd, J. T. & Vanhoutte, P. M. Am. J. Physiol. 252, H671–H673 (1987).

    CAS  PubMed  Google Scholar 

  10. DeMey, J. G. & Vanhoutte, P. M. J. Physiol., Lond. 335, 65–74 (1983).

    Article  CAS  Google Scholar 

  11. Daly, R. N. & Hieble, J. P. Eur. J. Pharmac. 138, 445–446 (1987).

    Article  CAS  Google Scholar 

  12. Hickey, K. A., Rubanyi, G., Paul, R. J. & Highsmith, R. F. Am. J. Physiol. 248, C550–C556 (1985).

    Article  CAS  PubMed  Google Scholar 

  13. Gillespie, M. N., Owasoyo, J. O., McMurtry, I. F. & O'Brien, R. F. J. Pharmac. exp. Ther. 236, 339–343 (1986).

    CAS  Google Scholar 

  14. O'Brien, R. F., Robbins, R. J. & McMurtry, I. F. J. cell. Physiol. 132, 263–270 (1987).

    Article  CAS  PubMed  Google Scholar 

  15. Vanhoutte, P. M., Rubanyi, G. M., Miller, V. M. & Houston, D. S. A. Rev. Physiol. 48, 307–320 (1986).

    Article  CAS  Google Scholar 

  16. Regoli, D., Park, W. K. & Rioux, F. Pharmac. Rev. 26, 69–123 (1974).

    CAS  Google Scholar 

  17. Altura, B. M. Am. J. Physiol. 219, 222–229 (1970).

    Article  CAS  PubMed  Google Scholar 

  18. Edvinsson, L. Acta physiol. scand. 125, 33–41 (1986).

    Article  Google Scholar 

  19. Kozak, M. Nucleic Acids Res. 12, 857–872 (1984).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Perlman, D. & Halvorson, H. O. J. molec. Biol. 167, 391–409 (1983).

    Article  CAS  PubMed  Google Scholar 

  21. Turner, A. J. Essays Biochem. 22, 69–119 (1986).

    CAS  PubMed  Google Scholar 

  22. Johnson, A. R. & Erdos, E. G. J. clin. Invest. 59, 684–695 (1977).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Holtz, J. et al. J. cardiovasc. Pharmac. 6, 1161–1169 (1984).

    Article  CAS  Google Scholar 

  24. Rubanyi, G. M., Romero, J. C. & Vanhoutte, P. M. Am. J. Physiol. 250, H1145–H1149 (1986).

    Article  CAS  PubMed  Google Scholar 

  25. Johannessen, J. V. Electron Microscopy in Human Medicine Vol. 5, 87–154 (McGraw–Hill, New York, 1980).

    Google Scholar 

  26. Habermann, E. Science 177, 314–322 (1972).

    Article  ADS  CAS  Google Scholar 

  27. Olivera, B. M. et al. Science 230, 1338–1343 (1985).

    Article  ADS  CAS  PubMed  Google Scholar 

  28. Fontecilla-Camps, J. C. et al. Proc. natn. Acad. Sci. U.S.A. 77, 6496–6500 (1980).

    Article  ADS  CAS  Google Scholar 

  29. Lipman, D. J. & Pearson, W. R. Science 227, 1435–1441 (1985).

    Article  ADS  CAS  PubMed  Google Scholar 

  30. Kopeyan, C., Martinez, G. & Rochat, H. FEBS Lett. 181, 211–217 (1985).

    Article  CAS  Google Scholar 

  31. Jover, E., Couraud, F. & Rochat, H. Biochem. biophys. Res. Commun. 95, 1607–1614 (1980).

    Article  CAS  PubMed  Google Scholar 

  32. Catterall, W. A. A. Rev. Biochem. 55, 953–985 (1986).

    Article  CAS  Google Scholar 

  33. Tanabe, T. et al. Nature 328, 313–318 (1987).

    Article  ADS  CAS  PubMed  Google Scholar 

  34. Vanhoutte, P. M. & Rimele, T. J. J. Physiol., Paris 78, 681–686 (1983).

    CAS  Google Scholar 

  35. Vanhoutte, P. M. Blood Vessels 24, 141–144 (1987).

    CAS  PubMed  Google Scholar 

  36. Ross, R. New Engl. J. Med. 314, 488–500 (1986).

    Article  CAS  PubMed  Google Scholar 

  37. Gajdusek, C. M. & Schwartz, S. M. In Vitro 19, 394–402 (1983).

    Article  CAS  PubMed  Google Scholar 

  38. von Heijne, G. Nucleic Acids Res. 14, 4683–4690 (1986).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  39. Dayhoff, M. O., Schwartz, R. M. & Orcutt, B. C. in Atlas of Protein Sequence and Structure Vol. 5, Suppl. 3 (ed. Dayhoff, M. O.) 345–352 (National Biomedical Research Foundation, Silver Spring, Maryland, 1978).

    Google Scholar 

  40. Maniatis, T., Fritsch, E. F. & Sambrook, J. Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory, New York, 1982).

    Google Scholar 

  41. Huynh, T. V., Young, R. A. & Davis, R. W. in DNA Cloning: A Practical Approach, Vol. 1 (ed. Glover, D. M.) 49–78 (IRL, Oxford, 1985).

    Google Scholar 

  42. Lathe, R. J. molec. Biol. 183, 1–12 (1985).

    Article  CAS  PubMed  Google Scholar 

  43. Lauffer, L. et al. Nature 318, 334–338 (1985).

    Article  ADS  CAS  PubMed  Google Scholar 

  44. Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

    Article  ADS  CAS  Google Scholar 

  45. Clemens, M. J. in Transcription and translation: A Practical Approach (ed. Hames, B. D. & Higgins, S. J.) 211–230 (IRL, Oxford, 1984).

    Google Scholar 

  46. Feinberg, A. P. & Vogelstein, B. Analyt. Biochem. 132, 6–13 (1983).

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yanagisawa, M., Kurihara, H., Kimura, S. et al. A novel potent vasoconstrictor peptide produced by vascular endothelial cells. Nature 332, 411–415 (1988). https://doi.org/10.1038/332411a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/332411a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing