Abstract
The polymorphonuclear leukocyte (PMN), or neutrophil, is the major host defence cell protecting the body against invasion by bacteria and fungi. Products of oxidative metabolism mediate PMN microbicidal and tumoricidal activity1 but the mechanisms by which these pathways become activated are not well understood. We have previously described a human granulocyte–macrophage colony-stimulating factor (GM-CSF) of relative molecular mass (Mr) 22,000 that also inhibits neutrophil motility (NIF-T activity)2. Because of its direct action on granulocytes, this lymphokine is a candidate for a neutrophil-activating factor. We have studied the effect of GM-CSF/NIF-T on Superoxide anion generation in response to the bacterial chemo-attractant N-formylmethionyl-leucylphenylalanine (f-MLP)3, and report here that PMNs preincubated with either purified natural GM-CSF or biosynthetic (recombinant) GM-CSF showed increased (as much as fourfold) Superoxide anion production in response to f-MLP. These results indicate that human GM-CSF is a neutrophil-activating factor.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Babior, B. M. New Engl. J. Med. 298, 659–668, 721–725 (1978).
Gasson, J. C. et al. Science 226, 1339–1342 (1984).
English, D., Roloff, J. S. & Lukens, J. N. J. Immun. 126, 165–171 (1981).
Weisbart, R. H., Golde, D. W., Spolter, L., Eggena, P. & Rinderknecht, H. Clin. Immun. Immunopath. 14, 441–448 (1979).
Weisbart, R. H. et al. Clin. Immun. Immunopath. 22, 408–418 (1982).
Weisbart, R. J. et al. J. Immun. 128, 457–462 (1982).
Rosen, H. & Klebanoff, S. J. J. clin. Invest. 58, 50–60 (1976).
Wong, G. G. et al. Science (in the press).
Lopez, A. F. et al. J. Immun. 131, 2983–2988 (1983).
Weisbart, R. H. & Mickey, M. R. J. immun. Meth. 16, 269–281 (1977).
Cline, M. J. & Golde, D. W. Nature 277, 177–181 (1979).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Weisbart, R., Golde, D., Clark, S. et al. Human granulocyte-macrophage colony-stimulating factor is a neutrophil activator. Nature 314, 361–363 (1985). https://doi.org/10.1038/314361a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/314361a0
This article is cited by
-
Serum amyloid P inhibits granulocyte adhesion
Fibrogenesis & Tissue Repair (2013)
-
Psoriatic keratinocytes prime neutrophils for an overproduction of superoxide anions
Archives of Dermatological Research (2013)
-
Effect of intramammary infusion of rbGM-CSF on SCC and expression of polymorphonuclear neutrophil adhesion molecules in subclinical mastitis cows
Veterinary Research Communications (2012)
-
High titer autoantibodies to GM-CSF in patients with AML, CML and MDS are associated with active disease
Leukemia (2008)
-
Granulocyte Colony-Stimulating Factor Mediates Cardioprotection Against Ischemia/Reperfusion Injury via Phosphatidylinositol-3-Kinase/Akt Pathway in Canine Hearts
Cardiovascular Drugs and Therapy (2006)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
