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Sequence and organization of the human mitochondrial genome

Abstract

The complete sequence of the 16,569-base pair human mitochondrial genome is presented. The genes for the 12S and 16S rRNAs, 22 tRNAs, cytochrome c oxidase subunits I, II and III, ATPase subunit 6, cytochrome b and eight other predicted protein coding genes have been located. The sequence shows extreme economy in that the genes have none or only a few noncoding bases between them, and in many cases the termination codons are not coded in the DNA but are created post-transcriptionally by polyadenylation of the mRNAs.

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References

  1. Borst, P. Trends biochem. Sci. 2, 31–34 (1977).

    Article  CAS  Google Scholar 

  2. Barrell, B. G., Bankier, A. T. & Drouin, J. Nature 282, 189–194 (1979).

    Article  ADS  CAS  Google Scholar 

  3. Young, I. G. & Anderson, S. Gene 12, 257–265 (1980).

    Article  CAS  Google Scholar 

  4. Li, M. & Tzagoloff, A. Cell 18, 47–53 (1979).

    Article  CAS  Google Scholar 

  5. Macino, G., Coruzzi, G., Nobrega, F. G., Li, M. & Tzagoloff, A. Proc. natn. Acad. Sci. U.S.A. 76, 3784–3785 (1979).

    Article  ADS  CAS  Google Scholar 

  6. Fox, T. D. Proc. natn. Acad. Sci. U.S.A. 76, 6534–6538 (1979).

    Article  ADS  CAS  Google Scholar 

  7. Heckman, J. E., Sarnoff, J., Alzner DeWeerd, B., Yyn, S. & RajBhandary, U. L. Proc. natn. Acad. Sci. U.S.A. 77, 3159–3163 (1980).

    Article  ADS  CAS  Google Scholar 

  8. Barrell, B. G. et al. Proc. natn. Acad. Sci. U.S.A. 77, 3164–3166 (1980).

    Article  ADS  CAS  Google Scholar 

  9. Bonitz, S. G. et al. Proc. natn. Acad. Sci. U.S.A. 77, 3167–3170 (1980).

    Article  ADS  CAS  Google Scholar 

  10. Crews, S., Ojala, D., Posakony, J., Nishiguchi, J. & Attardi, G. Nature 277, 192–198 (1979).

    Article  ADS  CAS  Google Scholar 

  11. Gillum, A. M. & Clayton, D. A. J. molec. Biol. 135, 353–368 (1979).

    Article  CAS  Google Scholar 

  12. Martens, P. A. & Clayton, D. A. J. molec. Biol. 135, 327–351 (1979).

    Article  CAS  Google Scholar 

  13. Aloni, Y. & Attardi, G. Proc. natn. Acad. Sci. U.S.A. 68, 1757–1761 (1971).

    Article  ADS  CAS  Google Scholar 

  14. Murphy, W., Attardi, B., Tu, C. & Attardi, G. J. molec. Biol. 99, 809–814 (1975).

    Article  CAS  Google Scholar 

  15. Attardi, G. et al. in Proc. ICN-UCLA Symp. Extrachromosomal DNA (1979) (in the press).

  16. Eperon, I. C., Anderson, S. & Nierlich, D. P. Nature 286, 460–467 (1980).

    Article  ADS  CAS  Google Scholar 

  17. Ojala, D., Montoya, J. & Attardi, G. Nature 290, 470–474 (1981).

    Article  ADS  CAS  Google Scholar 

  18. Montoya, J., Ojala, D. & Attardi, G. Nature 290, 465–470 (1981).

    Article  ADS  CAS  Google Scholar 

  19. Gillum, A. M. & Clayton, D. A. Proc. natn. Acad. Sci. U.S.A. 75, 677–681 (1978).

    Article  ADS  CAS  Google Scholar 

  20. Brown, W. M., Shine, J. & Goodman, H. M. Proc. natn. Acad. Sci. U.S.A. 75, 735–739 (1978).

    Article  ADS  CAS  Google Scholar 

  21. Bogenhagen, D. & Clayton, D. A. J. molec. Biol. 119, 49–68 (1978).

    Article  CAS  Google Scholar 

  22. Sekiya, T., Kobayashi, M., Seki, T. & Koike, K. Gene 11, 53–62 (1980).

    Article  CAS  Google Scholar 

  23. Upholt, W. B. & Dawid, I. B. Cell 11, 571–583 (1977).

    Article  CAS  Google Scholar 

  24. Berk, A. J. & Clayton, D. A. J. molec. Biol. 86, 801–824 (1974).

    Article  CAS  Google Scholar 

  25. Crews, S. & Attardi, G. Cell 19, 775–784 (1980).

    Article  CAS  Google Scholar 

  26. Van Etten, R. A., Walberg, M. W. & Clayton, D. A. Cell 22, 157–170 (1980).

    Article  CAS  Google Scholar 

  27. Baer, R. & Dubin, D. T. Nucleic Acids Res. 8, 4927–4941 (1980).

    Article  CAS  Google Scholar 

  28. Dubin, D. T., Timko, K. D. & Baer, R. J. Cell (in the press).

  29. Cantatore, P. & Attardi, G. Nucleic Acids Res. 8, 2605–2624 (1980).

    Article  CAS  Google Scholar 

  30. Baer, R. & Dubin, D. T. Nucleic Acids Res. 8, 4927–4941 (1980).

    Article  CAS  Google Scholar 

  31. Staden, R. Nucleic Acids Res. 8, 817–825 (1980).

    Article  CAS  Google Scholar 

  32. Aujame, L. & Freeman, K. B. Nucleic Acids Res. 6, 455–469 (1979).

    Article  CAS  Google Scholar 

  33. Crick, F. H. C. J. molec. Biol. 19, 548–555 (1966).

    Article  CAS  Google Scholar 

  34. Lagerkvist, U. Proc. natn. Acad. Sci. U.S.A. 75, 1759–1762 (1978).

    Article  ADS  CAS  Google Scholar 

  35. Lynch, D. & Attardi, G. J. molec. Biol. 102, 125–141 (1976).

    Article  CAS  Google Scholar 

  36. Wrede, P., Woo, N. H. & Rich, A. Proc. natn. Acad. Sci. U.S.A. 76, 3289–3293 (1979).

    Article  ADS  CAS  Google Scholar 

  37. Kuchino, Y., Watanabe, S., Harada, F. & Nishimura, S. Biochemistry 19, 2085–2089 (1980).

    Article  CAS  Google Scholar 

  38. Fukada, K. & Abelson, J. J. molec. Biol. 139, 377–391 (1980).

    Article  CAS  Google Scholar 

  39. de Bruijn, M. H. L. et al. Nucleic Acids Res. 8, 5213–5222 (1980).

    Article  CAS  Google Scholar 

  40. Grauss, D. H., Grüter, F. & Sprinzl, M. Nucleic Acids. Res. 6, r1–r19 (1979).

    Article  Google Scholar 

  41. Steffens, G. J. & Buse, G. Hoppe-Seyler's Z. physiol. Chem. 360, 613–619 (1979).

    CAS  PubMed  Google Scholar 

  42. Thalenfeld, B. E. & Tzagoloff, A. J. biol. Chem. 255, 6173–6180 (1980).

    CAS  PubMed  Google Scholar 

  43. Macino, G. & Tzagoloff, A. Cell 20, 507–517 (1980).

    Article  CAS  Google Scholar 

  44. Nobrega, F. G. & Tzagoloff, A. J. biol. Chem. 255, 9828–9837 (1980).

    CAS  Google Scholar 

  45. Sebald, W. & Wachter, E. in 29th Mossbacher Colloquium on Energy Conservation in Biological Membranes (eds Schafer, G. & Klingenberg, M.) 228–236 (Springer, Berlin, 1978).

    Book  Google Scholar 

  46. Hensgens, L. A., Grivell, L. A., Borst, P. & Bos, J. L. Proc. natn. Acad. Sci. U.S.A. 76, 1663–1667 (1979).

    Article  ADS  CAS  Google Scholar 

  47. Macino, G. & Tzagoloff, A. J. biol. Chem. 254, 4617–4623 (1979).

    CAS  PubMed  Google Scholar 

  48. Ojala, D., Markel, C., Gelfand, R. & Attardi, G. Cell 22, 393–403 (1980).

    Article  CAS  Google Scholar 

  49. Barrell, B. G. et al. in 31st Mosbach Colloquium (eds Bucher, Th., Weiss, H. & Sebald, W.) (Springer, Berlin, in the press).

  50. Robertson, M. D., Altman, S. & Smith, J. D. J. biol. Chem. 247, 5243–5251 (1972).

    CAS  PubMed  Google Scholar 

  51. Altman, S. & Smith, J. D. Nature new Biol. 233, 35–39 (1971).

    Article  CAS  Google Scholar 

  52. Attardi, G. et al. in The Organisation and Expression of the Mitochondrial Genome (eds Kroon, A. M. & Saccone, C.) (North-Holland, Amsterdam, in the press).

  53. Battey, J. & Clayton, D. A. J. biol. Chem. 255, 11599–11606 (1980).

    CAS  PubMed  Google Scholar 

  54. Borst, P. & Grivell, L. A. Cell 15, 705–723 (1978).

    Article  CAS  Google Scholar 

  55. Staden, R. Nucleic Acids Res. 4, 4037–4051 (1977); 5, 1013–1015 (1978).

    Article  CAS  Google Scholar 

  56. Drouin, J. J. molec. Biol. 140, 15–34 (1980).

    Article  CAS  Google Scholar 

  57. Smith, A. J. H. Nucleic Acids Res. 6, 831–848 (1979).

    Article  CAS  Google Scholar 

  58. Gronenborn, B. & Messing, J. Nature 272, 375–377 (1978).

    Article  ADS  CAS  Google Scholar 

  59. Schreier, P. H. & Cortese, R. J. molec. Biol. 129, 169–172 (1979).

    Article  CAS  Google Scholar 

  60. Sanger, F., Coulson, A. R., Barrell, B. G., Smith, A. J. H. & Roe, B. A. J. molec. Biol. 143, 161–178 (1980).

    Article  CAS  Google Scholar 

  61. Anderson, S., Gait, M. J., Mayol, L. & Young, I. G. Nucleic Acids Res. 8, 1731–1743 (1980).

    Article  CAS  Google Scholar 

  62. Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

    Article  ADS  CAS  Google Scholar 

  63. Sanger, F. & Coulson, A. R. FEBS Lett. 87, 107–110 (1978).

    Article  CAS  Google Scholar 

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Anderson, S., Bankier, A., Barrell, B. et al. Sequence and organization of the human mitochondrial genome. Nature 290, 457–465 (1981). https://doi.org/10.1038/290457a0

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