Abstract
The SV40 early region TATA box functions in vitro to fix initiation of transcription within a narrow region identical to that in vivo. In the absence of the TATA box region initiation occurs at multiple specific sites, as in vivo. However, certain sequences far upstream, crucial for efficient in vivo expression, are not essential in vitro.
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References
Weil, P. A., Luse, D. S., Segall, J. & Roeder, R. G. Cell 18, 469–484 (1979).
Manley, J. L., Fire, A., Cano, A., Sharp, P. A. & Gefter, M. L. Proc. natn. Acad. Sci. U.S.A. 77, 3855–3859 (1980).
Wasylyk, B., Kédinger, C., Corden, J., Brison, O. & Chambon, P. Nature 285, 367–373 (1980).
Luse, D. S. & Roeder, R. G. Cell 20, 691–699 (1980).
Corden, J. et al. Science 209, 1406–1414 (1980).
Gannon, F. et al. Nature 278, 428–434 (1979).
Proudfoot, N. J. Nature 279, 376 (1979).
Flavell, R. A. Nature 285, 356–357 (1980).
Breathnach, R. & Chambon, P. A. Rev. Biochem. (in the press).
Wasylyk, B. et al. Proc. natn. Acad. Sci. U.S.A. 77, 7024–7028 (1980).
Grosschedl, R. & Birnsteil, M. L. Proc. natn. Acad. Sci. U.S.A. 77, 1432–1436 (1980).
Benoist, C. & Chambon, P. Proc. natn. Acad. Sci. U.S.A. 77, 3865–3869 (1980).
Benoist, C. & Chambon, P. Nature, 290, 304–310 (1981).
Ghosh, P., Reddy, V., Swinscoe, J., Lebowitz, P. & Weissman, S. J. molec. Biol. 126, 813–846 (1979).
Handa, H., Kaufman, R. J., Manley, J., Gefter, M. & Sharp, P. A. J. biol. Chem. (in the press).
Hentschel, C., Irminger, J. C., Bucher, P. & Birnstiel, M. L. Nature 285, 147–151 (1980).
Weaver, R. F. & Weissman, C. Nucleic Acids Res. 7, 1175–1195 (1979).
Sollner-Webb, B. & Reeder, R. Cell 18, 485–499 (1979).
Tsujimoto, Y. & Suzuki, Y. Cell 16, 425–436 (1979).
Reddy, V. B., Ghosh, P. K., Lebowitz, P., Piatak, M. & Weissman, S. M. J. Virol. 30, 279–296 (1979).
Haegeman, G. & Fiers, W. J. Virol. 35, 955–961 (1980).
Gluzman, Y., Sambrook, J. F. & Frisque, R. J. Proc. natn. Acad. Sci. U.S.A. 77, 3898–3902 (1980).
Rosenberg, M. & Court, D. A. Rev. Genet. 13, 319–353 (1979).
Wasylyk, B., Oudet, P. & Chambon, P. Nucleic Acids Res. 7, 705–713 (1979).
Germond, J. E., Hirt, B., Oudet, P., Gross-Bellard, M. & Chambon, P. Proc. natn. Acad. Sci. U.S.A. 72, 1843–1847 (1975).
Birkenmeier, E. H., Radonovich, M. F., Shani, M. & Salzman, N. P. Cell 11, 495–504 (1977).
Van Heuverswyn, H. & Fiers, W. Eur. J. Biochem. 100, 51–60 (1979).
Maxam, A. & Gilbert, W. Meth. Enzym. 65, 499–580 (1980).
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Mathis, D., Chambon, P. The SV40 early region TATA box is required for accurate in vitro initiation of transcription. Nature 290, 310–315 (1981). https://doi.org/10.1038/290310a0
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DOI: https://doi.org/10.1038/290310a0
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