Abstract
Escherichia coli strains have been constructed in which lacZ, the gene for the cytoplasmic enzyme β-galactosidase, is fused to lamB, the gene for an outer membrane protein. One such strain produces a β-galactosidase which remains cytoplasmic even though it possesses the complete signal sequence of the lamB protein precursor at the amino-terminal end.
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Moreno, F., Fowler, A., Hall, M. et al. A signal sequence is not sufficient to lead β-galactosidase out of the cytoplasm. Nature 286, 356–359 (1980). https://doi.org/10.1038/286356a0
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DOI: https://doi.org/10.1038/286356a0
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