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Cloning and endonuclease mapping of the hepatitis B viral genome

Nature volume 279, pages 346–348 (1979)Cite this article

Abstract

THE virus that causes hepatitis B, or serum hepatitis, seems to infect only humans in nature, and experimental infection has been achieved in only a few additional mammals. The limited host range of the hepatitis B virus (HBV), and its failure so far to infect tissue culture cells have drastically restricted study of this virus and have hindered development of a vaccine for the serious disease that it causes. We report here the cloning of double-stranded HBV DNA in Escherichia coli K12, using the unique EcoRI cleavage site on the viral genome to introduce the entire HBV DNA molecule into an EcoRI cleavage site within the chloroamphenicol (Cm) resistance gene of the pACYC184 plasmid vector1.

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References

  1. Chang, A. C. Y. & Cohen, S. N. J. Bact. 134, 1141–1156 (1978).

    CAS  PubMed  PubMed Central  Google Scholar 

  2. Dane, D. S., Cameron, C. H. & Briggs, M. Lancet ii 695–698 (1970).

    Article  Google Scholar 

  3. Hruska, J. F., Clayton, D. A., Rubenstein, J. L. R. & Robinson, W. S. J. Virol. 21, 666–672 (1977).

    CAS  PubMed  PubMed Central  Google Scholar 

  4. Landers, T. A., Greenberg, H. B. & Robinson, W. S. J. Virol. 23, 368–376 (1977).

    CAS  PubMed  PubMed Central  Google Scholar 

  5. Summers, J. A., O'Connel, A. & Millman, I. Proc. natn. Acad. Sci. U.S.A. 72, 4597–4601 (1975).

    Article  ADS  CAS  Google Scholar 

  6. Robinson, W. S. A. Rev. Microbiol. 31, 357–377 (1977).

    Article  CAS  Google Scholar 

  7. Ullrich, A. et al. Science 196, 1313–1320 (1977).

    Article  ADS  CAS  PubMed  Google Scholar 

  8. Cohen, S. N., Chang, A. C. Y., Boyer, H. W. & Helling, R. B. Proc. natn. Acad. Sci. U.S.A. 70, 3240–3244 (1973).

    Article  ADS  CAS  Google Scholar 

  9. Wu, R., Jay, E. & Roychoudhury, R. Meth. Cancer Res. 12, 87–176 (1976).

    CAS  Google Scholar 

  10. Smith, W. O. & Birnsteil, M. L. Nucleic Acids Res. 3, 2387–2398 (1976).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Fritsch, V., Pourcel, C., Charnay, P. & Tiollais, P. C. r. hebd. Séanc. Acad. Sci., Paris 287, 1453–1456 (1978).

    CAS  Google Scholar 

  12. Kuperstoch, Y. M. & Helinski, D. R. Biochem. biophys. Res. Commun. 54, 1451–1459 (1973).

    Article  Google Scholar 

  13. Tanaka, N., Cramer, J. H. & Rownd, R. H. J. Bact. 127, 619–636 (1976).

    CAS  Google Scholar 

  14. Southern, E. M. J. molec. Biol. 98, 503–517 (1975).

    Article  CAS  PubMed  Google Scholar 

  15. Denhardt, D. T. Biochem. biophys. Res. Commun. 23, 641–646 (1966).

    Article  CAS  PubMed  Google Scholar 

  16. Maniatis, T., Jeffrey, A. & Kleid, D. G. Proc. natn. Acad. Sci. U.S.A. 72, 1184–1188 (1975).

    Article  ADS  CAS  Google Scholar 

  17. Rigby, P. W. J., Dieckmann, M., Rhodes, C. & Berg, P. J. molec. Biol. 113, 237–251 (1977).

    Article  CAS  PubMed  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Medicine, Stanford University, School of Medicine, Stanford, California, 94305

    JOHN J. SNINSKY, ALEEM SIDDIQUI, WILLIAM S. ROBINSON & STANLEY N. COHEN

  2. Department of Genetics, Stanford University, School of Medicine, Stanford, California, 94305

    JOHN J. SNINSKY, ALEEM SIDDIQUI & STANLEY N. COHEN

Authors
  1. JOHN J. SNINSKY
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  2. ALEEM SIDDIQUI
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  3. WILLIAM S. ROBINSON
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  4. STANLEY N. COHEN
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SNINSKY, J., SIDDIQUI, A., ROBINSON, W. et al. Cloning and endonuclease mapping of the hepatitis B viral genome. Nature 279, 346–348 (1979). https://doi.org/10.1038/279346a0

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