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The natural ovalbumin gene contains seven intervening sequences

Abstract

EcoRI fragments of the natural ovalbumin gene were cloned and studied by hybridisation with mature ovalbumin mRNA, electron microscopy, restriction enzyme mapping and limited sequence analysis. The structural gene sequences coding for ovalbumin were found to be separated into eight sequentially orientated pieces by seven intervening sequences of various lengths.

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References

  1. Botchan, T., Topp, W. & Sambrook, J. Cell 9, 269–287 (1976).

    Article  CAS  Google Scholar 

  2. Watson, J. D. in Molecular Biology of the Gene (W. A. Benjamin, Menlo Park, 1976).

    Google Scholar 

  3. Jeffreys, A. J. & Flavell, R. A. Cell 12, 1097–1108 (1977).

    Article  CAS  Google Scholar 

  4. Tilghman, S. M. et al. Proc. natn. Acad. Sci. U.S.A. 74, 4406–4409 (1977).

    Article  ADS  CAS  Google Scholar 

  5. Brack, C. & Tonegawa, S. Proc. natn. Acad. Sci. U.S.A. 74, 5652–5656 (1977).

    Article  ADS  CAS  Google Scholar 

  6. Glover, D. M. & Hogness, D. S. Cell 10, 167–176 (1977).

    Article  CAS  Google Scholar 

  7. White, R. L. & Hogness, D. S. Cell 10, 177–192 (1977).

    Article  CAS  Google Scholar 

  8. Wellauer, P. K. & Dawid, I. B. Cell 10, 193–212 (1977).

    Article  CAS  Google Scholar 

  9. Goodman, H. M., Olson, M. V. & Hall, B. D. Proc. natn. Acad. Sci. U.S.A. 74, 5453–5457 (1977).

    Article  ADS  CAS  Google Scholar 

  10. Valenzuela, P., Venegas, A., Weinberg, F., Bishop, R. & Rutter, W. J. Proc. natn. Acad. Sci. U.S.A. 75, 190–194 (1978).

    Article  ADS  CAS  Google Scholar 

  11. Breathnach, R., Mandel, J. L. & Chambon, P. Nature 270, 314–319 (1977).

    Article  ADS  CAS  Google Scholar 

  12. Doel, M. T., Houghton, M., Cook, E. A. & Carey, N. H. Nucleic Acids Res. 4, 3701–3713 (1977).

    Article  CAS  Google Scholar 

  13. Lai, E. C., Woo, S. L. C., Dugaiczyk, A., Catterall, J. F. & O'Malley, B. W. Proc. natn. Acad. Sci. U.S.A. 75, 2205–2209 (1978).

    Article  ADS  CAS  Google Scholar 

  14. Dugaiczyk, A. et al. in Int. Symp. genet. Engng, Milan, March, 1978 (in the press).

    Google Scholar 

  15. Weinstock, R., Sweet, R., Weise, M., Cedar, H. & Axel, R. Proc. natn. Acad. Sci. U.S.A. 75, 1299–1303 (1978).

    Article  ADS  CAS  Google Scholar 

  16. McReynolds, L. et al. Nature 273, 723–728 (1978).

    Article  ADS  CAS  Google Scholar 

  17. McReynolds, L. A., Catterall, J. F. & O'Malley, B. W. Gene 2, 217–231 (1977).

    Article  CAS  Google Scholar 

  18. Maniatis, T., Jeffrey, A. & Kleid, D. G. Proc. natn. Acad. Sci. U.S.A. 72, 1184–1188 (1975).

    Article  ADS  CAS  Google Scholar 

  19. Hardies, S. C. & Wells, R. D. Proc. natn. Acad. Sci. U.S.A. 9, 3117–3121 (1976).

    Article  ADS  Google Scholar 

  20. Leder, P., Tiemeier, D. & Enquist, L. Science 196, 175–177 (1977).

    Article  ADS  CAS  Google Scholar 

  21. Tiemeier, D. C., Tilghman, S. M. & Leder, P. Gene 2, 173–191 (1977).

    Article  CAS  Google Scholar 

  22. Benton, W. D. & Davies, R. W. Science 196, 180–182 (1977).

    Article  ADS  CAS  Google Scholar 

  23. Woo, S. L. C. et al. Proc. natn. Acad. Sci. U.S.A. (in the press).

  24. Dugaiczyk, A., Boyer, H. W. & Goodman, H. M. J. molec. Biol. 96, 171–184 (1975).

    Article  CAS  Google Scholar 

  25. Southern, E. M. J. molec. Biol. 98, 503–517 (1977).

    Article  Google Scholar 

  26. Garapin, A. C. et al. in Int. Symp. genet. Engng, Milan, March 1978 (in the press).

    Google Scholar 

  27. Alwine, J. C., Kemp, D. J. & Stark, G. R. Proc. natn. Acad. Sci. U.S.A. 74, 5350–5354 (1977).

    Article  ADS  CAS  Google Scholar 

  28. O'Malley, B. W., McGuire, W. L., Kohler, P. K. & Korenman, S. G. in Recent Progress in Hormone Research, Vol. 13, 105–160 (Academic, New York, 1969).

    Google Scholar 

  29. O'Malley, B. W. & Means, A. R. Science 183, 610–620 (1974).

    Article  ADS  CAS  Google Scholar 

  30. Schrader, W. T. & O'Malley, B. W. in Receptors and Hormone Action, Vol. 2, 189–224 (Academic, New York, 1978).

    Book  Google Scholar 

  31. O'Malley, B. W., Towle, H. C. & Schwartz, R. J. A. Rev. Genet. 11, 239–275 (1977).

    Article  CAS  Google Scholar 

  32. Helling, R. B., Goodman, H. M. & Boyer, H. W. J. Virol. 14, 1235–1244 (1974).

    CAS  PubMed  PubMed Central  Google Scholar 

  33. Dingman, C. W., Fisher, M. P. & Kakefuda, T. Biochemistry 11, 1242–1250 (1972).

    Article  CAS  Google Scholar 

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Dugaiczyk, A., Woo, S., Lai, E. et al. The natural ovalbumin gene contains seven intervening sequences. Nature 274, 328–333 (1978). https://doi.org/10.1038/274328a0

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