Abstract
MANY human lymphoblastoid cell lines have been established from subjects with different haemopoietic disorders as well as from normal individuals1–3. In most cases the inability to identify the precise origin of cells which have been established has limited the usefulness of these lines, especially those derived from leukaemic donors. Although there are a few important exceptions to this generalisation (2, 3, 4 and see below) it is significant that no lines have been established from the common form of acute lymphoblastic leukaemia (ALL) which is usually referred to as ‘null’ cell or ‘non-T, non-B’ ALL (refs 5–7). We describe here the establishment of a lymphoid cell line, Reh, derived from an acute lymphocytic leukaemia where the leukaemic identity of established cells is supported by kinetic data, chromosome markers and detection of leukaemia associated antigens and enzymes.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Moore, G. E. & Minowada, J. in Hemic Cells in Vitro (ed. Fames, P.) 110 (The Williams and Wilkins, Baltimore, 1969).
Nilsson, K. & Ponten, J., Int. J. Cancer 15, 321 (1975).
Zech, L., Haglund, U., Nilsson, K. & Klein, G., Int. J. Cancer 17, 47 (1976).
Minowada, J., Tsubota, T., Greaves, M. F. & Walters, T. R., J. natn. Cancer Inst. (in the press).
Brouet, J.-C. et al. Br. J. Haematol. 33, 319 (1976).
Greaves, M. F., Progr. Haematol. 9, 255 (1975).
Collins, R. D., Smith, J. L., Clein, G. P., Br. J. Haematol. 26, 615 (1974).
Rosenfeld, C., Macieira-Coelho, A., Venuat, A. M., Jasmin, C. & Tuan, T. Q., J. natn. Cancer Inst. 43, 581 (1969).
Ehlenberger, A. G. & Nussenzweig, V. in In Vitro Methods of Cell Mediated and Tumor Immunity (eds Bloom, B. & David, J.) 113 (Academic, New York, 1976).
Thierfelder, S., Rodt, H. & Thiel, E. (eds) Immunological Diagnosis of Leukaemias and Lymphomas (Lehmanns, Munich and New York).
Special WHO–IUIS technical report Br. J. Haematol. (in the press).
Greaves, M. F. in Immunological Diagnosis of Leukaemias and Lymphomas (eds Thierfelder, S., Rodt, H. & Thiel, E.) 61 (Lehmanns, Munich and New York, 1977).
Brown, G., Capellaro, D. & Greaves, M. F., J. Natn. Cancer Inst. 55, 1281 (1975).
Schlossman, S., Chess, L., Humphreys, R. E. & Strominger, J. L., Proc. natn. Acad. Sci. U.S.A. 73, 1288 (1976).
McCaffrey, R., Harrison, T. A., Parkman, R. & Baltimore, D., New Engl. J. Med. 292, 775 (1975).
Kaplan, J., Shope, T. C. & Peterson, W. D., J. exp. Med. 139, 1070 (1974).
Minowada, J. et al. in Immunological Diagnosis of Leukaemias and Lymphomas (eds Thierfelder, S., Rodt, H & Thiel, E.) (Lehmanns, Munich and New York, in the press).
Janossy, G., Roberts, M. & Greaves, M. F., Lancet ii, 1058 (1976).
Schneider, U., Schwenk, H. U. & Bornkamm, G. in Immunological Diagnosis of Leukaemias and Lymphomas 265 (eds Thierfelder, S., Rodt, H. & Thiel, E.) (Lehmanns, Munich and New York, 1977).
Gunz, F. & Baikie, A. G. (eds) Leukaemia (Grune and Stratton, New York, 1974).
Seeker-Walker, L. M. et al. Lancet ii, 1405 (1976).
Greaves, M. F. in Immunodiagnosis of Cancer (ed. Herbermann, R.) (Academic, New York, in the press).
Mathé, G. et al. Proc. R. Soc. Med. 68, 211 (1975).
Brown, G., Hogg, N. & Greaves, M. F., Nature 258, 454 (1975).
Janossy, G., Greaves, M. F., Sutherland, R., Durrant, J. & Lewis, C., Leukemia Research 2, (in the press).
Greaves, M. F. & Janossy in In Vitro Methods in Cell Mediated and Tumor Immunity (eds Bloom, B & David, J.) (Academic, New York, 1976).
Greaves, M. F., Falk, J. & Falk, R., Scand. J. Immun. 4, 80 (1975).
Baker, R., Falk, J., Falk, R. & Greaves, M. F., Br. J. Haematol. 32, 13 (1976).
Catovsky, D., Cherchi, M., Okos, A., Hegde, O. & Galton, D. A. G., Br. J. Haematol. 33, 173(1976).
Stathopolos, G. & Davies, A. J. S., Lancet i, 1078 (1976).
Bloom, B. & David, U. (eds) In Vitro Methods of Cell Mediated and Tumor Immunity (Academic, New York, 1976).
Ellis, R. B., Ikonne, J. U. & Masson, P. K., Analyt. Biochem. 63, 5 (1975).
Hutton, J. J. & Coleman, M. S., Br. J. Haematol. 37, 447 (1976).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
ROSENFELD, C., GOUTNER, A., CHOQUET, C. et al. Phenotypic characterisation of a unique non-T, non-B acute lymphoblastic leukaemia cell line. Nature 267, 841–843 (1977). https://doi.org/10.1038/267841a0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/267841a0
This article is cited by
-
Genomic, transcriptomic and epigenomic sequencing data of the B-cell leukemia cell line REH
BMC Research Notes (2023)
-
EP300-ZNF384 fusion gene product up-regulates GATA3 gene expression and induces hematopoietic stem cell gene expression signature in B-cell precursor acute lymphoblastic leukemia cells
International Journal of Hematology (2017)
-
A reliable Raman-spectroscopy-based approach for diagnosis, classification and follow-up of B-cell acute lymphoblastic leukemia
Scientific Reports (2016)
-
Mechanisms of clonal evolution in childhood acute lymphoblastic leukemia
Nature Immunology (2015)
-
Hypermethylation and down-regulation of DLEU2 in paediatric acute myeloid leukaemia independent of embedded tumour suppressor miR-15a/16-1
Molecular Cancer (2014)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.