Abstract
THERE have been several attempts to define conditions necessary for the fixation of the transformed state in mammalian cells in vitro. The relationship between fixation and proliferation after treatment has been studied in cell cultures exposed to methylcholanthrene1, X irradiation2,3 and SV40 virus4. Cells were exposed to the carcinogen while in the density-inhibited plateau phase of growth, and subsequently subcultured at low cell density so that they could resume cell division. Transformation frequency was always reduced by delaying subculture for 24 h or longer, suggesting that cells must divide soon after exposure to a carcinogen in order to fix the transformed state. No data were reported for subculture less than 24 h after treatment.
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TERZAGHI, M., LITTLE, J. Repair of potentially lethal radiation damage in mammalian cells is associated with enhancement of malignant transformation. Nature 253, 548–549 (1975). https://doi.org/10.1038/253548a0
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DOI: https://doi.org/10.1038/253548a0
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