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Induced Optical Activity of 2,4-Dinitrophenyl-lysine Specifically Bound to Mouse MOPC-315 Myeloma Protein

Nature volume 232, pages 192–194 (1971)Cite this article

Abstract

THE optical activity generated by the interaction of small ligands with proteins, visible as extrinsic optical rotatory dispersion (ORD) Cotton effects or circular dichroism (CD) bands, has been used extensively to study the binding of ligands to non-antibody proteins1,2. We describe here the use of circular dichroism to monitor the binding of DNP-lysine to a mouse IgA myeloma protein with high affinity for dinitrophenylated haptens3–5.

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Author information

Author notes

  1. JOHN M. ROCKEY

    Present address: Department of Ophthalmology, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania

  2. KEITH J. DORRINGTON

    Present address: Department of Biochemistry, University of Toronto,

  3. PAUL C. MONTGOMERY

    Present address: Department of Microbiology, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania

Authors and Affiliations

  1. MRC Molecular Pharmacology Unit, Medical School, Hills Road, Cambridge

    JOHN M. ROCKEY & KEITH J. DORRINGTON

  2. National Institute for Medical Research, Mill Hill, London, NW7

    PAUL C. MONTGOMERY

Authors
  1. JOHN M. ROCKEY
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  2. KEITH J. DORRINGTON
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  3. PAUL C. MONTGOMERY
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ROCKEY, J., DORRINGTON, K. & MONTGOMERY, P. Induced Optical Activity of 2,4-Dinitrophenyl-lysine Specifically Bound to Mouse MOPC-315 Myeloma Protein. Nature 232, 192–194 (1971). https://doi.org/10.1038/232192a0

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  • DOI: https://doi.org/10.1038/232192a0

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