Abstract
Using a rapid one-step method, circular DNA has been isolated from a strain of E. coli harbouring the R-factor R-1 and shown to contain at least two separate classes of covalently closed molecules. Evidence is presented for the stable existence of circular R-factor in E. coli.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Watanabe, T., Bact. Rev., 27, 87 (1963).
Falkow, S., Citarella, R. V., Wohlheiter, J. A., and Watanabe, T., J. Mol. Biol., 17, 102 (1966).
Rownd, R., Nakaya, R., and Nakamura, A., J. Mol. Biol, 17, 176 (1966).
Anderson, E. S., Ann. Rev. Microbiol., 22, 131 (1968).
Watanabe, T., and Fukasawa, T., J. Bact., 82, 202 (1961).
Anderson, E. S., and Lewis, M. J., Nature, 208, 843 (1965).
Nisioka, T., Mitani, M., and Clowes, R., J. Bact., 97, 376 (1969).
Cohen, S. N., Bact. Proc., 69, 49 (1969).
Vinograd, J., Lebowitz, J., Radloff, R., Watson, R., and Laipis, P., Proc. US Nat. Acad Sci., 53, 1104 (1965).
Radloff, R., Bauer, W., and Vinograd, J., Proc. US Nat. Acad. Sci., 57, 1514 (1967).
Nygaard, A. P., and Hall, B. D., J. Mol. Biol., 9, 125 (1964).
Kornberg, A., Zimmerman, S. B., Kornberg, S. R., and Voose, J., Proc. US Nat. Acad. Sci., 45, 772 (1959).
Cohen, S. N., and Hurwitz, J., J. Mol. Biol., 37, 387 (1968).
Godson, G. N., and Sinsheimer, R. L., Biochim. Biophys. Acta, 149, 476 (1967).
Davern, C. I., Proc. US Nat. Acad. Sci., 55, 792 (1966).
Bazaral, M., and Helinski, D. R., J. Mol. Biol., 36, 185 (1968).
Jansz, H., Pouwels, P. S., and Schiphorst, J., Biochim. Biophys. Acta, 123, 626 (1967).
Bazaral, M., and Helmski, D. R., Biochemistry, 10, 3513 (1968).
Studier, F. W., J. Mol. Biol., 11, 373 (1965).
Burgi, E., Proc. US Nat. Acad. Sci., 49, 151 (1963).
Eigner, J., in Methods in Enzymology, 386 (Academic Press, New York, 1968).
Falkow, S., Haapala, D., and Silver, R. P., in Bacterial Episomes and Plasmids, 136 (Churchill London 1969).
Klemschmidt, A. K., Lang, D., Jacherts, D., and Zahn, R. K., Biochim. Biophys. Acta, 61, 857 (1962).
Inman, R. B., Schildkraut, C. L., and Kornberg, A., J. Mol. Biol., 11, 285 (1965).
MacHattie, L. A., Berns, K. I., and Thomas, jun, C. A., J. Mol. Biol., 11, 648 (1965).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
COHEN, S., MILLER, C. Multiple Molecular Species of Circular R-factor DNA isolated from Escherichia coll. Nature 224, 1273–1277 (1969). https://doi.org/10.1038/2241273a0
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1038/2241273a0
This article is cited by
-
Plasmid R46 provides a function that promotes recA-independent deletion, fusion and resolution of replicon
Molecular and General Genetics MGG (1984)
-
Production of extrachromosomal r-determinant circles from integrated R100.1: Involvement of the E. coli recombination system
Molecular and General Genetics MGG (1980)
-
Coupled chromatography on Sepharose 4B-cellulose nitrate columns for the isolation of an R-plasmid fromPseudomonas aeruginosa
Current Microbiology (1979)
-
The construction and replication properties of hybrid plasmids composed of the r-determinant of R100.1 and the plasmids pCRI or pSC201
Molecular and General Genetics MGG (1979)
-
Involvement of IS1 in the dissociation of the r-determinant and RTF components of the plasmid R100.1
Molecular and General Genetics MGG (1977)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
