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Crystals of Fragment Fab′: Preparation from Pepsin Digests of Human IgG Myeloma Proteins

Abstract

INFORMATION concerning the three-dimensional structure of an antibody and the contours of its combining site can in theory be derived by X-ray crystallography. In view of the large molecular weight of antibody molecules (150,000 or greater), crystals of fragment Fab, which has a molecular weight of 50,000 and contains the combining site of an antibody, may be of particular value.

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ROSSI, G., CHOI, T. & NISONOFF, A. Crystals of Fragment Fab′: Preparation from Pepsin Digests of Human IgG Myeloma Proteins. Nature 223, 837–838 (1969). https://doi.org/10.1038/223837a0

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  • DOI: https://doi.org/10.1038/223837a0

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