Abstract
A COMPARISON between the optical rotatory dispersion curves of rhodopsin before and after irradiation has been previously reported1. Unbleached rhodopsin solutions show a weak Cotton effect in the visible range, and another distinguishable Cotton effect in the ultra-violet range, attributable to the polypeptide conformation (probably α-helix) of the protein moiety, opsin. After bleaching, however, the samples have a simple dispersion curve showing lower values of optical rotation in the visible range. The Cotton effect in the visible range is thus presumably caused by the optical activity of the prosthetic-group conformation (11-cis), although this is not certain. We have made measurements of the circular dichroism of rhodopsin and isorhodopsin (the artificial pigment with a prosthetic group in the 9-cis configuration) in order to elucidate the weak Cotton effect demonstrated by optical rotatory dispersion.
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TAKEZAKI, M., KITO, Y. Circular Dichroism of Rhodopsin and Isorhodopsin. Nature 215, 1197–1199 (1967). https://doi.org/10.1038/2151197a0
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DOI: https://doi.org/10.1038/2151197a0
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