Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Monolayer Cultures of Insect Cell Lines and their Inoculation with a Plant Virus

Abstract

TWENTY-SEVEN years after Trager1 reported short term survival and growth of silkworm cells from dissected female gonads, Grace successfully established continuous insect cell lines in vitro from ovarian tissues excised from diapausing pupae of Antheraea eucalypti2. The various attempts, during the interim, to improve in vitro growth of insect cells have been critically reviewed3–5.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Trager, W., J. Exp. Med., 61, 501 (1935).

    Article  CAS  Google Scholar 

  2. Grace, T. D. C., Nature, 195, 788 (1962).

    Article  ADS  CAS  Google Scholar 

  3. Day, M. F., and Grace, T. D. C., Ann. Rev. Entomol., 4, 17 (1959).

    Article  CAS  Google Scholar 

  4. Martignoni, M. E., Experientia, 16, 125 (1960).

    Article  CAS  Google Scholar 

  5. Jones, B. M., Biol. Rev. (British), 37, 512 (1962).

    Article  CAS  Google Scholar 

  6. Vago, C., and Flandre, O., Ann. Epiphyties 14 (Numero hors series 3), 127 (1963).

  7. Mitsuhashi, J., and Maramorosch, K., Contr. Boyce Thompson Inst., 22, 435 (1964).

    Google Scholar 

  8. Hirumi, H., and Maramorosch, K., Exp. Cell Res., 36, 625 (1964).

    Article  CAS  Google Scholar 

  9. Mitsuhashi, J., Jap. J. Appl. Entomol. Zool., 9, 107 (1965).

    Article  Google Scholar 

  10. Chiu, R-j., Reddy, D. V. R., and Black, L. M., Virology, 30, 562 (1966).

    Article  CAS  Google Scholar 

  11. Maramorosch, K., Mitsuhashi, J., Streissle, G., and Hirumi, H., Bacteriol. Proc., 120 (1965).

  12. Mitsuhashi, J., Jap. J. Appl. Entomol. Zool., 9, 137 (1965).

    Article  Google Scholar 

  13. Hirumi, H., and Maramorosch, K., Contr. Boyce Thompson Inst., 22, 343 (1964).

    Google Scholar 

  14. Schneider, I., J. Exp. Zool., 156, 91 (1964).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

CHIU, RJ., BLACK, L. Monolayer Cultures of Insect Cell Lines and their Inoculation with a Plant Virus. Nature 215, 1076–1078 (1967). https://doi.org/10.1038/2151076a0

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1038/2151076a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing