Abstract
ALL forms of radioimmunoassay used at present include a procedure to separate free and bound tracer antigen when equilibrium is reached in the incubation mixture. This separation may involve precipitation by a second antibody1,2, electrophoresis3,4, chromatoelectrophoresis5, ion exchange6, solvent fractionation7, gel filtration8,9, salt precipitation10, or adsorption to charcoal11. The need to use such formal isolation procedures increases the length and complexity of the assay and introduces a number of possible sources of error.
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CATT, K., NIALL, H. & TREGEAR, G. Solid Phase Radioimmunoassay. Nature 213, 825–827 (1967). https://doi.org/10.1038/213825a0
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DOI: https://doi.org/10.1038/213825a0
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