Abstract
WE are trying to dissolve native proteins from wool by the method of limited proteolysis1,2. Unreduced wool is not attacked by trypsin and so, in our experiments, wool has been reduced under the mildest possible conditions which will allow trypsin digestion. We believe that these conditions do not cause denaturation of the wool3. Apparently, large protein fragments are dissolved from the reduced wool by trypsin, and in a synthetic boundary run4 in the Spinco model E ultracentrifuge, the unfractionated solution gave a schlieren peak, sedimentation coefficient of 3.1. One obstacle to further characterization of the dissolved proteins is the enzyme in the solution, which may continue to digest the fragments it has released from the fibre. This communication describes experiments which show that the inhibitor phenylmethylsulphonylfluoride (PMSF)5 will arrest the reaction of trypsin with reduced wool.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Linderstrøm-Lang, K., Abst. Proc. Ninth Solvay Congress, 1 (Brussels, 1953).
Greenwood, B. D., and Speakman, P. T., Nature, 204, 144 (1964).
Jordan, B. J., and Speakman, P. T., IIIe Congrès Intern. de la Recherche Textile Lainière, Paris, 1965, 1, 117.
Greenwood, B. D., and Speakman, P. T., Nature, 205, 79 (1965).
Fahrney, D. E., and Gold, A. M., J. Amer. Chem. Soc., 85, 997 (1963).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
SPEAKMAN, P., YARWOOD, R. Trypsin Inhibition by Phenylmethylsulphonylfluoride with Reduced Wool as Substrate. Nature 211, 201–202 (1966). https://doi.org/10.1038/211201b0
Issue Date:
DOI: https://doi.org/10.1038/211201b0
This article is cited by
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.