Abstract
DURING the course of purification of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) from horse pituitary glands1 the following method was used to determine the degree of separation of FSH and LH at two purification steps. A pH-5 soluble gonadotropic fraction and a purified FSH preparation from the third zone-electrophoresis were compared as to their effect on the increase in the weight of ovaries as a measure of FSH and ventral prostates as a measure of LH in hypophysectomized rats (Holtzman Co., Madison, Wisconsin). A dose-response relationship was developed between the ovarian weights obtained by different doses of the pH-5 soluble fraction. The doses of the FSH fraction from the third zone-electrophoresis were selected so as to induce essentially the same ovarian response as was obtained with various doses of the pH-5 soluble fraction. The average weights of the ventral prostates produced by these doses were used as a measure of the LH content of the two fractions. The results are presented in Fig. 1 and Table 1.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Saxena, B. B., McShan, W. H., and Meyer, Roland K., Biochim. Biophys. Acta, 65, 394 (1962).
Goldstein, A., Biostatistics, 144 (Macmillan Co., New York, 1964).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
SAXENA, B. Bioassay of Pituitary Gonadotropins. Nature 207, 1198–1199 (1965). https://doi.org/10.1038/2071198a0
Published:
Issue Date:
DOI: https://doi.org/10.1038/2071198a0
This article is cited by
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.