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Effect of Charcoal on the Behaviour of Adenine Nucleotides in Ion-Exchange Chromatography

Abstract

CHARCOAL has been used widely for selective adsorption of nucleotides from reaction mixtures and tissue preparations. In experiments reported here three commercially available grades were all found to adsorb quantitatively and to elute reasonably satisfactorily the mono-, di- and tri-phosphates of adenosine; these charcoals were ‘Norit’, two batches of purified, activated charcoal supplied by Hopkin and Williams, Ltd., and a gift sample of ‘Ultrasorb ZX’ from British Carbo Norit Union, Ltd.

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PETHIYAGODA, U. Effect of Charcoal on the Behaviour of Adenine Nucleotides in Ion-Exchange Chromatography. Nature 192, 452–454 (1961). https://doi.org/10.1038/192452a0

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