Abstract
THE albumin sedimentation method of Vallee et al. 1 has been applied to the problem of isolating tumour cells from the circulating blood2. This method and its modifications involve the collection of the cells at an interface. When a variety of cells together with platelets are packed at an interface they are apt to sink or float as a unit. Such artefacts may seriously interfere with separation in a centrifugal field3. Moreover, we have found the cells to show morphological changes after prolonged centrifugation in contact with a foreign solution. It is therefore desirable to use a gradient method4.
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References
Vallee, B. L., Hughes, W. L., and Gibson, J. G., Blood (Special Issue), 1, 82 (1947).
Roberts, S., Watne, A., McGrath, R., McGrew, E., and Cole, W. H., Amer. Med. Assoc. Arch. Surg., 76, 334 (1958).
Anderson, N. G., Exp. Cell Res., 9, 446 (1955).
Graham, H. T., Lowry, O. H., Wheelwright, F., Lenz, M. A., and Parish, jun., H. H., Blood, 10, 467 (1955).
Alexander, R. F., and Spriggs, A. I., J. Clin. Path., 13, 414 (1960).
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SPRIGGS, A., ALEXANDER, R. An Albumin Gradient Method for separating the Different White Cells of Blood, applied to the Concentration of Circulating Tumour Cells. Nature 188, 863–864 (1960). https://doi.org/10.1038/188863a0
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DOI: https://doi.org/10.1038/188863a0
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