Abstract
GLUCOSE-1-PHOSPHATE and glucose-6-phosphate are not readily separated by the solvent systems normally used for the analysis of the naturally occurring phosphoric esters on paper chromatograms (for example, propanol – ammonia – water, t-butanol – picric acid – water, etc.1). Though very similar in structure, the esters differ in that glucose-6-phosphate has two cis-hydroxyl groups available for substitution and the addition of boric acid to some of the solvent systems previously described enables glucose-6-phosphate to be differentiated clearly from glucose-1-phosphate2.
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References
Hanes, C. S., and Isherwood, F. A., Nature, 164, 1107 (1949).
Isherwood, F. A., British Medical Bulletin, 10 (3), 202 (1954).
Mortimer, D. C., Can. J. Chem., 30, 653 (1952).
Isherwood, F. A., and Hanes, C. S., Biochem. J., 55, 284 (1953).
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HARRAP, F. Separation of Glucose-1-Phosphate and Glucose-6-Phosphate by Paper Chromatography. Nature 182, 876 (1958). https://doi.org/10.1038/182876a0
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DOI: https://doi.org/10.1038/182876a0
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